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Biomedical subjects

T Goda

Publications and source records attributed to T Goda.

124 records · Page 7Linked to original sources
PubMed1982

Effect of sucrose and Acarbose feeding on the development of streptozotocin-induced diabetes in the rat.

The effects of sucrose and Acarbose (alpha-glucosidase inhibitor) feeding on the development of diabetes were studied in streptozotocin-treated rats. Rats were raised on four different dietary regimens, viz, a sucrose diet (46% of the total weight in the form of sucrose, 24% as starch), a starch diet (70% as starch), a standard diet (laboratory chow: Oriental Yeast Co.) or an Acarbose diet (a standard diet containing 75 mg Acarbose/100g diet) for a week followed by an intraperitoneal injection of streptozotocin (70 mg/kg). Development of diabetes was determined by urinary and blood glucose levels (more than 250 mg/dl). The incidence of diabetes in the groups of rats fed on sucrose, starch, standard, and Acarbose diets was 100%, 80%, 70% and 47.6%, respectively. The development of diabetes was accelerated by sucrose feeding and depressed by Acarbose feeding. There was mild diabetes in rats fed on Acarbose diet. The sucrose feeding caused a marked increase of disaccharidase activities in the proximal part of the intestine and in the apical part of the villus-crypt gradient of epithelial cells. The Acarbose feeding caused a significant decrease of disaccharidase activities. The changes in protein content of the sucrase-isomaltase complex appeared to be in parallel with those of disaccharidase activities. These results suggest that intestinal disaccharidase activities are involved in the development of experimental diabetes induced by streptozotocin.

Acarbose↗
PubMed1982

Structures of ATP(adenosine triphosphate)-vanadyl complexes.

The state of existence of cytoplasmic vanadium ion is known to be important: vanadyl ion forms complexes with ATP and vanadate form inhibits (Na++K+)-ATPase. Therefore, the formation of complexes between ATP and vanadyl ion was investigated. Formations of three types of complex were observed: a blue 1:1 complex formed in acidic pH region; a blue 1:1 complex formed in neutral pH region; a green 2:1 complex formed in alkaline pH region. On the basis of the results on potentiometric titration, optical and EPR spectra and 31P- and 13C-NMR spectra, three characteristic types of coordination environment are proposed.

Adenosine Triphosphate↗
PubMed1980

Effect of food restriction on intestinal disaccharidases in streptozotocin-induced diabetes of rat.

The effect of food restriction on the intestinal weight and membrane digestive enzyme activities was observed in rats with diabetes induced by streptozotocin. The specific activities of disaccharidases of food non-restricted diabetic rats were not changed, but total activities were significantly increased due to the increase of intestinal mucosal weight. Restriction of food intake did not increase intestinal mucosal weight, but significantly increased the specific and total activities of disaccharidases in parallel with the increase of sucrase-isomaltase content. On the other hand, experimental diabetes did not influence the activity of alkaline phosphatase and leucine aminopeptidase except for specific and total activities of alkaline phosphatase in food non-restricted rats.

Alkaline Phosphatase↗
PubMedDate not supplied

Thrombopoietin (TPO) levels in hepatic patients with thrombocytopenia.

Thrombocytopenia is a common problem complicating the course of liver disease. One of the postulated mechanisms in chronic liver disease is impaired production of the hormone, thrombopoietin (TPO). The aim of present study was to evaluate the role of TPO on the occurrence of thrombocytopenia. Serum TPO levels was determined by ELISA in 40 patients with liver disease (11 seropositive with hepatitis C; 10 with mixed liver cirrhosis; 19 with bilharzial hepatic fibrosis), plus 14 normal healthy subjects as a control group. The sTPO levels were unevenly distributed among the liver disease subgroups being the highest in the group with HCV (median 1232.0, range 154.7-2042.0 pg/ml) followed by the mixed cirrhosis group (556.5; 342.0-1497.0 pg/ml) and lowest among the bilharzial hepatic fibrosis group (130.0; 22.0-204.0 pg/ml) (P<0.01). While sTPO levels in HCV and cirrhotic group were significantly higher when compared to the control group (97.0; 19.0-377.0 pg/ml), those in the Bilharzial hepatic fibrosis group were not significantly elevated (P>0.05). There is significant negative correlation between sTPO levels and spleen size (R=-0.3, P=0.043); but there was no correlation with platelet count (R=0.09, P>0.05). In addition, sTPO levels were significantly higher in patients with platelet counts >or=60x10(9)/l as compared to those with platelet counts <60x10(9)/l (P=0.04). Using the receiver operating curve (ROC) at sTPO cut off value >or= vs. <368 ng/ml, most of HCV and cirrhotic patients had higher sTPO levels (81.8 and 80.0%, respectively), while all Bilharzial hepatic fibrosis group (100%) had lower sTPO levels. In conclusion, sTPO levels had no role in the occurrence of thrombocytopenia in liver disease patients and other factors appear to be more important. It also appears that the mechanism controlling sTPO levels might be different in cirrhotic patients compared to Bilharzial hepatic fibrosis patients.

Adult↗
PubMedDate not supplied

Effect of diet on intestinal and pancreatic enzyme activities in the pig.

Intestinal and pancreatic enzyme activities are known to respond to changes in dietary composition. Studies in rats and humans suggest that adaptive mechanisms differ between species in response to altered intakes of carbohydrate and fat. Because of increased use of the pig in the study of human nutrition, we compared the responses of pancreatic enzymes and intestinal disaccharidases in groups of 7- to 10-week-old pigs fed either high-carbohydrate/low-fat (70 cal% starch, 25% protein, 5% fat) or low-carbohydrate/high-fat (5, 25, 70%, respectively) diets for 7 and 30 days. No changes were observed in the activities for lactase, trypsin, or chymotrypsin or in the tissue protein concentrations, regardless of diet duration. High-carbohydrate/low-fat intake resulted in higher specific activities of sucrase, maltase, and amylase for both periods studied. Low-carbohydrate/high-fat intake resulted in higher specific activities of pancreatic lipase for both periods studied. The response of the intestinal disaccharidases differs from that observed previously in rodents but resembles the response reported in humans. Conversely, amylase and lipase responded similarly to the pattern in the rat. These data support the continued use of the pig as a suitable model in the study of adaptation to altered intakes of carbohydrate and fat.

Animals↗