Search PubMed⌕ Search

Biomedical subjects

T Gentile

Publications and source records attributed to T Gentile.

51 records · Page 3Linked to original sources
PubMed1986

IgG precipitating and non-precipitating antibodies in rabbits repeatedly injected with soluble and particulate antigens.

The immune response of precipitating antibodies and non-precipitating antibodies of high affinity (co-precipitating) of the IgG class was analyzed in rabbits repeatedly injected with egg albumin (as a soluble antigen) B. abortus-egg albumin and polymerized egg albumin (as particulate antigens). The results showed that the levels of anti-egg albumin non-precipitating antibodies induced by the soluble antigen were never higher than 10-15% of total antibodies throughout the experimental time. When particulate antigens were injected, the levels of non-precipitating antibodies increased up to 30-70% of the total antibody levels. This phenomenon is related to the way in which the antigen is available to the immune system (particle or aggregated), and is independent of the response induced by the particulate carrier. Components from the cell wall or bacterial membrane that could act as coadjuvants do not participate in this phenomenon. The results obtained seem to indicate that possibly there was a suppression of the synthesis of precipitating antibodies, and this would produce a relative increase in the non-precipitating antibodies.

Agglutination↗
PubMed1985

Decay B--> psi X.

Explore the source record for details and available documents.

Journal Article↗
PubMed1982

[Production of coprecipitating antibody in rabbits immunized repeatedly with ovalbumin in different physical states].

A comparative study has been made of rabbit precipitating and coprecipitating anti-egg albumin antibody in rabbits repeatedly injected with soluble and particulate antigens. Four different antigens were used. a) Soluble egg albumin. b) Polymerized egg albumin. c) Egg albumin linked Brucella abortus 19 strain. d) Brucella abortus 19 strain. Rabbits were subcutaneously injected every 2 weeks during 29 weeks. Before each inoculation they were bled and the concentration of serum precipitating and coprecipitating antibodies was determined. In animals inoculated with the soluble antigen, coprecipitating antibodies were present during the whole course of the immune response and they constituted 10% of the total antibody population. In rabbits injected with particulate antigen (egg albumin linked Brucella abortus) coprecipitating antibodies were also present during the whole course of the immune response but their serum concentration was not the same in different periods of time. An increase in coprecipitating antibodies began at the 13th week reaching 50% of the total antibodies at the 28th week. Similar results had been obtained in rabbits injected with Brucella s. p. In animals inoculated with polymerized egg albumin, coprecipitating antibodies increased to high levels from the 1st to the 23rd week; during this period it constituted 50% of the total antibody population, after which, the animals became tolerogenic. On the basis of these results, the increase in coprecipitating antibodies in rabbit sera would be related to: 1) antigen physicochemical characteristics (soluble o particulate); 2) the number of epitopes in the particle, and 3) the size of the antigen particles.

Animals↗
PubMed1980

Immunobiological behaviour of rabbit precipitating and non-precipitating (co-precipitating) antibodies.

Complement fixing capacity, antibody-dependent cell cytotoxicity and in vivo opsonic function of rabbit anti-DNP precipitating and co-precipitating antibodies were analysed. Precipitating antibody activated the complement system, but co-precipitating antibody did not. Both antibody preparations behaved similarly in antibody-dependent cytotoxicity reactions. When chicken erythrocytes were employed as target cells, both antibodies were inactive with homologous (rabbit) lymphocytes as effector cells but were active with heterologous lymphoid effector cells (human, guinea-pig). Clearance of antigen from the blood of mice was accelerated following injection of an optimal dose of precipitating antibody but co-precipitating antibody was ineffective even at different doses. Competition between precipitating and co-precipitating antibodies in complement fixation and antigen elimination following interaction with antigen successively or at the same time was investigated. In these tests, both antibodies competed with antigen by mass but not by affinity. The possibility exists that co-precipitating antibody has one high and one low affinity binding site and thus acts as an univalent blocking antibody and is therefore not able to form active complexes with antigen. The possible role of co-precipitating antibody in some humoral and cellular immune mechanisms is discussed.

Animals↗
PubMedDate not supplied

[The treatment with biosynthetic growth hormone (GH) of familial short stature].

The effect of growth hormone (GH) treatment in non-GH deficient subjects has been amply studied over the past few years. Although the results of these studies are encouraging, there are still no definitive data since the findings are not comparable due to the different characteristics of the populations examined. In the present study the authors examined the following parameters: stature, height SDS, growth rate, bone age and final height prediction according to Tanner, pubertal stage, before and after treatment with biosynthetic GH at a dose of 1.4 IU/kg of bodyweight for 12 months. The population treated consisted of 10 subjects (5 males and 5 females) aged between 7.3 and 9.5 years old, all prepubertal, with "familial short stature", selected according to the following criteria: stature below the 3rd centile, normal growth rate, normal GH response to stimuli using clonidine and insulin, correlation with parental stature between 25th and 75th centile, bone age correlated to chronological age, absence of other pathologies. After 12 months height SDS moved from -2.75 +/- 0.26 to -2.23 +/- 0.25 (p < 0.5); the growth rate changed from 5.75 +/- 0.63 to 6.66 +/- 0.56 (p < 0.05). No abnormal acceleration of bone age as observed: it moved from 8.2 +/- 0.62 to 9.5 +/- 0.72; all subjects continued to be prepubertal. The expected final stature changed from 154 +/- 2.38 to 159 +/- 0.7 (p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Age Determination by Skeleton↗