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Biomedical subjects

T Genda

Publications and source records attributed to T Genda.

27 records · Page 2Linked to original sources
PubMed1990

[Effect of maternal oxygenation on fetal blood gas status in shiba goat fetuses].

The purpose of this study is to investigate the effect of maternal oxygen administration on the fetal blood gas status. Five Shiba goat fetuses were used whose gestational days were 134 +/- 4 days. Maternal and fetal pH, pO2, pCO2, B.E., and hematocrit were measured every 30 minutes during 4 l/min oxygen administration for 60 minutes. Maternal arterial blood pO2 increased from 95.5 +/- 19.6 mmHg to 262.6 +/- 75.8 mmHg at 30 minutes and to 207.8 +/- 110.1 mmHg at 60 minutes. Fetal arterial blood pO2 increased significantly from 18.4 +/- 3.0 mmHg to 24.4 +/- 5.8 mmHg and 23.6 +/- 6.9 mmHg, respectively. There was a significant positive correlation between maternal pO2 and fetal pO2. The increase in fetal pO2 was small when the control pO2 was less than 17 mmHg. Although maternal pH did not change significantly, fetal pH decreased significantly from 7.29 +/- 0.07 to 7.25 +/- 0.07 at 60 minutes. Also fetal hematocrit decreased significantly from 33.9 +/- 5.2% to 32.8 +/- 5.4% at 60 minutes. Neither maternal nor fetal pCO2 and B.E. changed significantly. Therefore, we concluded that the effect of maternal oxygen administration on fetal pO2 was small when the fetus was already hypoxic. Also 60 minutes' oxygen administration may cause the decrease in fetal pH and hemodilution.

Animals↗
PubMed1989

[Changes in urinary immunoreactive-TXB2 and 2,3-dinor TXB2 in man following cardiopulmonary bypass].

We measured urinary levels of i-TXB2, 2,3-dinor TXB2 and NAG following cardiopulmonary bypass (CPB) and studied their clinical significance. Subjects studied were 8 patients undergoing cardiac surgery. In the CABG group, urinary i-TXB2, 2,3-dinor TXB2 and NAG level increased significantly (P less than 0.05) 3hrs after CPB, but decreased on and after the 1st postoperative day. In the DVR group, these levels showed similar changes, but i-TXB2 and NAG levels were significantly (P less than 0.05) higher than those of the CABG on the 1st or 3rd postoperative day. A significant correlation (r = 0.84, P less than 0.05) was observed between NAG and i-TXB2 on and after 2nd postoperative day. The results suggest that urinary i-TXB2 during the period immediately after CPB is derived from platelets and reflects the functional changes in renal urinary tubules, while urinary i-TXB2 level on and after the 2nd postoperative day reflects the function of the renal urinary tubules.

Acetylglucosaminidase↗
PubMedDate not supplied

Beneficial effect of fluorocarbon reperfusion on postoperative cardiac dysfunction of transplanted heart.

Fluosol DA 20% (Fluosol) perfusion was used to protect ischemic donor hearts of mongrel dogs from reperfusion injury. Fifteen orthotopically transplanted hearts, eight in the control group and seven in the Fluosol group, were studied for 3 hours after weaning from cardiopulmonary bypass. Donor hearts were arrested and immersed in 4 degrees C St. Thomas's Hospital Solution for 4 hours. The mean total ischemic time was 323 minutes (range, 298 to 345 minutes). In the Fluosol group, 200 ml of oxygenated Fluosol (37 degrees C; PO2 650 mm Hg; PCO2 35 mm Hg) was infused into the aortic root at approximately 100 ml/min just before aortic unclamping. Coronary sinus blood was analyzed for the MB fraction of creatine kinase, reduced glutathione, and oxidized glutathione. Hemodynamic and biochemical results were obtained at 30 minutes, 1 hour, and 3 hours after bypass. In the control group, during the second 30 minutes of the period after bypass, left ventricular end-diastolic pressure and stroke volume showed progressive deterioration, 54.8% increased (p less than 0.01) and 28.4% decreased (p less than 0.05), respectively. The MB fraction of creatine kinase and oxidized glutathione were increased, and reduced glutathione had declined, from 39.3 to 135.3 IU/L (p less than 0.01), from 28.0 to 33.4 micrograms/ml (p less than 0.05) and from 4.4 to 2.5 micrograms/ml (p less than 0.01), respectively. These parameters failed to recover during the next 2 hours, and massive mitochondrial degeneration was observed by electron microscopy. In the Fluosol group, these parameters maintained their baseline values, and electron microscopy showed well-preserved mitochondria. The data suggested that, in the control group, initial mitochondrial dysfunction was profound, persistent for at least 3 hours, and associated with membrane hyperpermeability, leading to cardiac dysfunction. Oxygenated Fluosol perfusion better preserved cardiac and mitochondrial function.

Animals↗