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Biomedical subjects

T G Taylor

Publications and source records attributed to T G Taylor.

At least 55 records · Page 3Linked to original sources
PubMed1977

An improved method for the microbiological assay of available amino acids in proteins using Tetrahymena pyriformis.

1. A standard microbiological procedure for the assay of available amino acids in proteins using Tetrahymena pyriformis has been modified by evaluating cell growth from the tetrahymanol content of the cultures by gas-liquid chromatography (GLC). 2. This modification improves the precision of the method compared with cell-counting techniques and provided automatic injection facilities are available for the GLC, the procedure is not excessively time-consuming. 3. It has been confirmed that enzymic predigestion is essential for all except the most soluble proteins and a satisfactory method using pronase has been incorporated into the microbiological procedure. 4. After enzymic digestion of the sample, growth of Tetrahymena with many proteins can be measured turbidimetrically, but particulate matter still persists with a proportion of samples, particularly those of plant origin, and the GLC method is therefore more widely applicable. It is also more precise. 5. Good agreement has been obtained between a chick bioassay and the modified Tetrahymena assay for available lysine in eight different proteins, including heat-damaged fish meals. 6. The modified procedure appeared to give good results for available tryptophan and methionine. 7. When cystine was omitted from the standard culture medium it was possible to test for available methionine plus cystine, but further work is required to assess the reliability of this particular assay.

Amino Acids↗
PubMed1977

The effects of ergosterol on the response of female chicks to oral oestrogens and progestogens.

The chick-oviduct assay was used to investigate the effects of dietary ergosterol on the response to oral progestogens and oestrogens. 2. Progestogens alone had no effect on the oviduct but the hypertrophy due to oestrogen was greatly enhanced by simultaneous treatment with progestogen at all dose levels tested. 3. Ergosterol had no effect on any of the responses of the oviduct studied.

Animals↗
PubMed1976

Effects of ergosterol on bone mineralisation in chicks given cholecalciferol or ergocalciferol.

Groups of chicks were given diets containing cholecalciferol or ergocalciferol supplemented with 0, 0-1, 1 or 10 g ergosterol/kg. 2. Ergosterol had no significant effect on growth, on the plasma concentration of calcium or on the content of bone-ash, indicating that it did not impair the absorption of either form of vitamin D. 3. An explanation is given for the apparent disagreement in the published findings on the relative anti-rachitic potencies of ergocalciferol and cholecalciferol in the chick.

Animals↗
PubMed1976

Glycerol release in vitro from adipose tissue of obese (ob/ob) mice treated with thyroid hormones.

Glycerol release from adipose tissue of adult genetically obese (ob/ob) mice was greater than that from adipose tissue of lean litter-mates. However, a higher concentration of adrenaline was required to stimulate lipolysis in ob/ob adipose tissue. Since oxygen consumption and the uptake of radioactive iodine by the thyroid were reduced and the biological half-life of thyroidal radio-iodine was lengthened, it is suggested that ob/ob mice are hypothyroid. The poor sensitivity of ob/ob adipose tissue to low doses of adrenaline was improved by treatment with either tri-iodothyronine or thyroxine. The lipolytic responses to both dibutyryl 3':5'-cyclic adenosine monophosphate and aminophylline were enhanced in thyroid hormone-treated lean and ob/ob mice. A higher dose of tri-iodothyronine was required to enhance lipolysis in ob/ob mice than in lean controls. The possibility that hypothyroidism is responsible for the change in response of ob/ob adipose tissue to lipolytic hormones is discussed.

Adipose Tissue↗