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Biomedical subjects

T Fukushima

Publications and source records attributed to T Fukushima.

At least 361 records · Page 20Linked to original sources

Extradural temporopolar approach to lesions of the upper basilar artery and infrachiasmatic region.

Surgical access to the parasellar, infrachiasmatic, and posterior clinoid regions has traditionally been accomplished through an intradural pterional or subtemporal approach. However, for large or complex lesions in these locations, such traditional trajectories may not afford sufficient exposure for complete obliteration of the pathological process. The authors describe an anterolateral transcavernous approach to this region that includes the following components: 1) extradural removal of the sphenoid wing and exposure of the superior orbital fissure and foramen rotundum; 2) removal of the anterior clinoid process via the anterolateral route; 3) decompression of the optic canal; 4) extradural retraction of the temporal tip; 5) transcavernous mobilization of the carotid artery and third cranial nerve; and 6) removal of the posterior clinoid process. This method results in enhanced exposure with minimal brain retraction and preservation of the temporal tip bridging veins. This approach has been used in 22 patients: 10 with basilar top aneurysms, eight with craniopharyngiomas, one with a tuberculum sellae meningioma, and two with trigeminal neuromas; the last patient had a carotid-cavernous fistula and a concomitant pituitary adenoma. Complete clip ligation was performed for all 10 basilar artery aneurysms, and gross total resection was achieved with preservation of the pituitary stalk in all tumor cases. Microscopic total resection was not possible in two cases of craniopharyngioma due to hypothalamic invasion. Two patients suffered transient postoperative hemiparesis, and one patient has persisting weakness; however, no patient followed for more than 6 months suffered any persistent cranial nerve morbidity. It is concluded that this procedure can serve as an alternative to either the transsylvian or subtemporal approaches when cranial base pathologies are large or complex.

Adolescent↗

Cranioplasty with the Medpor porous polyethylene flexblock implant. Technical note.

The authors describe the use of a porous polyethylene Flexblock implant for cosmetic cranioplasty. The implant may be used to cover any small- or medium-sized (< 8 cm) cranial defect, offering similar cosmetic results to standard alloplast cranioplasty while decreasing operation time. The porous implant design permits ingrowth of soft tissue and bone to increase implant strength and decrease the risk of infection. The Flexblock alloplast has been utilized in 25 cases with excellent cosmetic results and no implant-related complications.

Biocompatible Materials↗

Chromophobe renal cell carcinoma: a report of two cases.

Chromophobe renal cell carcinoma (RCC) is a recently established subtype of RCC, which has rarely been reported in Japan. In this communication, the authors report two Japanese cases of chromophobe RCC together with the immunohistochemical findings. The tumors were composed of sheets and cribriform glands formed by tumor cells with cloudy and reticular cytoplasm. Ultrastructurally, the cytoplasm was filled with numerous microvesicles. The tumor cells were positive for cytokeratin, epithelial membrane antigen, and Tamm-Horsfall protein. Occasionally, LeuM1-positive cells were also noted. Vimentin was negative, unlike the usual RCC. Reactivity for peanut agglutinin was more frequent than that to Lotus tetragonolobus agglutinin. The results of this study suggest that the tumor cells possessed phenotypes similar to the distal nephron rather than to the proximal tubular cells.

Adult↗

Modulation of ionic currents through GABAA receptor subtypes by endogenous steroids.

GABAA receptors were expressed in Xenopus oocytes by injecting messenger RNAs from chick retina and chick cortex, and the potency of 3 alpha-hydroxy-5 alpha-pregnan-20-one (3 alpha-OH-DHP) and 5 alpha-pregnane-3 alpha, 21-diol-20-one (THDOC) was investigated by electrophysiology under voltage clamp conditions. For the receptors of chick retina, 3 alpha-OH-DHP (100 nM) and THDOC (100 nM) augmented GABA actions (peak current) 1.6 times (n = 6) and 1.5 times (n = 7), respectively. The currents induced by 0.3-10 microM GABA for the receptors of the chick retina were sensitive to bicuculline, pentobarbital, and diazepam. GABAA receptors in the chick retina in vivo as well as the receptors in the brain may be functionally modulated by endogenous steroids.

Animals↗

Multistep alterations of human lymphocytes infected with HTLV-I and then exposed to carcinogens.

Normal human lymphocytes achieved continuous growth in IL-2-containing medium in vitro following HTLV-I infection. They showed specific structural abnormalities of certain chromosomes. Subsequent to exposure to MNNG and TPA, or to UV, they achieved autonomous growth (transformation). Alterations in the signal transduction system were observed in transformed cells that showed loss of ability to grow in the medium containing TPA. Additional chromosome abnormalities appeared in transformed cells. These abnormalities were found to cluster to chromosomes that had preexisting structural abnormalities. Changes of the met gene on chromosome 7, to which structural abnormalities were observed clustering, were detected by Southern blot analysis. Environmental carcinogens may work as cofactors to promote transformation of human lymphocytes infected with HTLV-I. The initial change of human lymphocytes caused by HTLV-I integration may be advanced toward neoplastic transformation by their exposure to other carcinogens.

Carcinogens↗

[Anesthetic management of a patient with 21-hydroxylase deficiency].

21-hydroxylase deficiency was described as a fatal disease in infancy manifested by severe adrenal insufficiency with lack of differentiation of the external genitalia in males. Patients are in adrenal insufficiency or receiving long term corticosteroid therapy and are unable to respond normally to surgery or other forms of stress. Therefore, supplementation with corticosteroids and various regimens are required for surgery and anesthetic management. A 3 year 10 months correction of 6] old phenotypic female [correction of male] with 21-hydroxylase deficiency and congenital adrenal hyperplasia was scheduled for critroplasty. Anesthesia was induced with sevoflurane, nitrous oxide and oxygen. After endotracheal intubation, a caudal epidural catheter was placed. At the induction of anesthesia, the patient received a bolus of hydrocortisone 30 mg i.v. followed by a continuous infusion of hydrocortisone. The patient's general condition was good during surgery without any major complications. Caudal epidural anesthesia is considered to be effective for anesthetic management of these patients.

Adrenal Hyperplasia, Congenital↗

[ABO genotyping of fingerprints by the PCR-RFLP method].

DNA typing of the ABO blood groups from single fingerprints was examined. Genomic DNAs were extracted from fingerprints on slide glasses by a simple method with NaI and N-lauroyl-sarcocinate, and then subjected to the PCR (Polymerase chain reaction). Two PCR fragments spanning positions 258 and 700 of the cDNA sequence of the ABO locus were amplified. Then, the PCR products were digested with a restriction enzyme, Kpn-1 or Msp-1, for each site. The digested PCR products were analyzed by electrophoresis on polyacrylamide gels for RFLP (Restriction-fragment length polymorphism). Bands on the gel were visualized by silver staining. Genotyping from single fingerprints after treatment with aluminium powder was also possible by these techniques.

ABO Blood-Group System↗

[Localization of platelet-derived endothelial cell growth factor in human glioblastoma and meningioma].

Localization of platelet-derived endothelial cell growth factor (PD-ECGF) in the surgical specimens of 11 human glioblastomas and 12 meningiomas was immunohistochemically examined with a polyclonal anti-PD-ECGF rabbit IgG. PD-ECGF was mainly localized in macrophages distributing around blood vessels at the peripheries of tumor tissue, especially of glioblastoma. PD-ECGF-positive macrophages were frequently accumulated in the vascular-rich stroma of glioblastoma, where occasionally expressed proliferating cell nuclear antigen-positive endothelial cells. However, few macrophages expressing PD-ECGF were scatteringly seen in meningioma. These findings suggest that PD-ECGF plays an important role in the growth of glioblastoma by affecting the stromal angiogenesis.

Brain Neoplasms↗

Genetic instability of human cells with DNA related to human retrovirus.

To elucidate the role of HTLV-I in human neoplasia, DNA was extracted from tumor tissues, cultured cells, and/or sera from 20 HTLV-I seropositive and from 10 seronegative cancer patients, subjected to PCR-PAGE, and analyzed by Southern hybridization. HTLV-I related sequences were detected in tumors of the seropositives with different types of cancer. In the seronegatives, gag related sequences were detected in some tumors, whereas LTR related sequences were detected in only uterus carcinoma and tax related sequences in none of tumors examined. These sequences were detected in tumor cells as well as lymphoid cells by in situ hybridization. Some of these sequences were also detected in cultured fibroblasts derived from ATL patients. Cultured normal human lymphocytes grew continuously in conditioned media following HTLV-I infection, and transformed on exposure to carcinogens. Chromosome changes in transformed cells appeared clustering to chromosomes abnormalized on HTLV-I infection. The frequency of abnormal chromosomes in lymphocytes was significantly higher in the seropositives and in their family members than in seronegative normal donors. The frequency increased with advancement of host age in the seropositives but not in the seronegatives. These findings indicate that some individuals carry HTLV-I related sequences that may be derepressed by host aging and resulted in increasing genetic instability of host cells rendering them increasingly susceptible to carcinogens.

Adult↗

[The effect of priming dose of vecuronium on the diaphragm and the hypothenar muscle].

We studied the relaxation property of priming or precurarization vecuronium dose (0.015 mg.kg-1) on the diaphragm and the abductor digiti minimi muscle in 8 healthy patients undergoing elective surgery. Patients had control ventilation (PETCO2: 35 - 40 mmHg) by the laryngeal mask under "thiopental-fentanyl-oxygen" anesthesia. Supra-maximal single twitch stimulation with 1 Hz frequency was applied to right phrenic and ulnar nerves. Electromyographic response was recorded using NEC Synax ER1100 (NEC, Sanei, Tokyo, Japan) by surface electrodes in the 8 th intercostal space and the hypothenar. Time to maximum blockade at the diaphragm was 189 +/- 37 sec, compared with 314 +/- 39 at the abductor digiti minimi muscle (P < 0.01). This result suggests that we should be careful about the respiratory state of primed or precurarized patients with small dose of vecuronium such as 0.015 mg.kg-1 administration.

Adult↗

[A 55-year-old man with prostate cancer, papilledema, and multiple cranial nerve palsies].

We report a 55-year-old man with papilledema and multiple cranial nerve palsies. He was well until 52 years of age when there was an onset of progressive difficulty in initiating urination; he visited the urology service of our hospital where a diagnosis of prostate cancer was made; the cancer was invading the bladder and was metastasizing to lymph nodes and bones. He was treated with oochiectomy and estrogen preparations with some improvement in his symptoms. Two years later, he developed difficulty in urination again, and transurethral resection of the tumor was performed in 1991. In December 1991, he noted tingling and numb sensation in his left face, which had become progressive worse within the next one month, and he developed blepharoptosis and deafness all on the left side. He was admitted to the urology service on February 4, 1992, and a neurological consultation was asked. On physical examination, general findings were unremarkable, except for lymph node enlargements of about 0.5 to 1.0 mm in size in cervical and inguinal regions. On neurologic examination, he was alert with normal mental activities; higher cerebral functions were intact. He had normal vision and visual fields, however, papilledema was present bilaterally; pupils and light reactions were normal. Extraocular muscles were intact on the right side, however, moderate restriction was noted in the left eye in that all the extraocular muscles except for the medial rectus were weak; blepharoptosis was noted on the left; no nystagmus was present. The sensation was diminished in the left face, and left facial paresis of the peripheral type was also noted; the taste sensation was also diminished in the left anterior two thirds of the tongue. He had sensorineural deafness on the left side. The other cranial nerves appeared intact. He walked normally; no weakness or muscle atrophy was noted; muscle tone was normal and no ataxia was observed. Deep reflexes were normally elicited and symmetric; the plantar response was flexor. No meningeal signs were present. Laboratory examination revealed following abnormalities: Hb 7.1 g/dl, platelet 47,000/cmm, WBC3,800/cmm, LDH 950IU/l, PAP232ng/ml (normal less than 1.6), PA2.631ng/ml (normal less than 7.4); a small amount of effusion was noted in the left pleural cavity; cytological examination of the fluid was class V. A cranial CT scan as well as MRI were entirely normal, as was the spinal tap. He was treated with glycerol, however, there was progressive increase in the pleural effusion, and he developed dyspnea; moist rale had become audible in the end of February.(ABSTRACT TRUNCATED AT 400 WORDS)

Adenocarcinoma↗

[Abscess formation in a Rathke's cleft cyst].

A 66-year-old woman with a diagnosis of asymptomatic Rathke's cleft cyst had been observed for almost 2 years and was admitted to our hospital in June 1992 because of high fever and loss of consciousness. Ophthalmological examination revealed right Horner's sign and ophthalmoplegia. A tentative diagnosis of bacterial meningitis was made based on the results of a lumbar puncture. T1-weight MRI showed a marked increase in the size of the suprasellar cystic mass lesion compared to the MRI images obtained 2 years earlier. Hence, the preoperative diagnosis was pituitary abscess. Both transsphenoidal and nasal drainage of the abscess were performed. The pathological findings were consistent with a Rathke's cleft cyst and showed chronic inflammatory cells. The postoperative course was uneventful and the patients eye movements recovered completely. The possible mechanisms of abscess formation in Rathke's cleft cyst are discussed.

Aged↗

[The morphological study of cerebrospinal fluid drainage at monkey arachnoid granulations].

It is generally accepted that the arachnoid granulations play an essential role in the drainage of the cerebrospinal fluid (CSF) into venous system, but there is continuing in controversy as to the morphological features of their functions. The present study was undertaken to investigate the structure and function of monkey arachnoid granulations under the increased pressure of subarachnoid space by light, scanning (SEM) and transmission (TEM) electron microscopy. Granulations were classified into three types by surface endothelial morphology. They were, 1) a granulation covered with bulged endothelium only, 2) a granulation covered with flatten endothelium only, and 3) a granulation covered with both two previous mentioned endothelia. Type 1) and 2) were spherical or hemispherical in shape. Type 3) granulations were lobulated, having the flatten endothelium at the constricted portion and the bulged endothelium in the other major portion. Three kinds of mechanisms for CSF drainage at arachnoid granulations ("closed system", "open system" and "temporary vacuolar transcellular channel") were proved to exist concurrently, because morphological criteria findings of each mechanism were observed in the same specimen. So, we proposed that these arachnoid granulations should be called "arachnoid granulations complexs".

Animals↗

[Treatment of acute lymphoblastic leukemia in adults by the modified protocol of L-10M (Sloan-Kettering): long-term outcome].

From January 1984 to April 1988, we treated 20 patients with adult lymphoblastic leukemia (ALL) and 2 patients with lymphoblastic lymphoma with a protocol which we modified L-10M of Sloan-Kettering Cancer Center. Since the median follow up time is now over 5 years, we report the most recent outcome. Thirteen patients were male and 9 were female. The median age was 31, ranging from 15 to 71 years of age, and there were no Ph1 positive patients. The complete remission (CR) rate was 81.8%. Median CR duration was 32 months and the 5-year continuous CR rate was 33.3%. No significant prognostic factor for CR rate was found. Age at achievement CR and duration were significant prognostic factors. The 5-year continuous CR rate of patients below 35 years old was 54.5%. In this group the leukocyte count was a significant prognostic factor. All patients with a leukocyte count above 1 x 10(4)/microliters relapsed. However, in patients with a WBC below 1 x 10(4)/microliters, the 5-year continuous CR rate was 75%. Based on these results, it seems reasonable to treat patients with ALL by different therapeutic strategies according to the risk factors.

Adolescent↗