Search PubMed⌕ Search

Biomedical subjects

T Fukuda

Publications and source records attributed to T Fukuda.

At least 1,495 records · Page 83Linked to original sources
PubMedDate not supplied

The importance of warming at 37 degrees C for removal of leukocytes and platelets from reconstituted red cells.

Red cells suspended in sodium phosphate buffer (R-RBC) were incubated at 37 degrees C, and the distribution of the leukocytes and platelets in the suspension was compared to a similar suspension incubated at 22 degrees C. Following centrifugation, a more dense packing of the red cells was observed in warm R-RBC, and less leukocytes and platelets remained in the red cells below the buffy coat, than was observed in R-RBC packed at room temperature. These experiments indicate that incubation at 37 degrees C prior to separation improves the quality of leukocyte- and platelet-depleted red cells.

Blood Platelets↗
PubMedDate not supplied

Platelet factor 4 release from the platelets stored in platelet concentrates.

Many consider that granular components of platelets should remain intact during storage of platelet concentrates (PCs) in order to participate in hemostasis when infused. The present investigations were done to determine factors affecting the release of an alpha-granular component, platelet factor 4 (PF-4), from platelets stored as PCs. Sedimentation of the pelleted platelet button and stronger flat-bed agitation induced a significant PF-4 release. After 1 day of storage at 22 degrees C, the percent PF-4 release correlated roughly with a pH fall in PCs (r = 0.520, y = 0.014 + 7.204). Rates of pH fall were roughly proportional to the platelet count. In addition, there was a positive relationship between PF-4 release and the platelet count per unit volume (r = 0.615, y = 0.045 + 1.327). The results indicated that one of the critical factors which determines the amount of PF-4 release during storage is concentrated pH. Lowering the platelet count per unit volume in PCs is better for the maintenance of granular components in platelets during storage because of the intact platelet metabolism.

Adolescent↗
PubMedDate not supplied

The formalin test: effects of formalin concentration and short-term halothane inhalation.

BACKGROUND AND OBJECTIVES: In order to evaluate the effects of formalin concentration and inhalational anesthetics in formalin tests, we injected 5 concentrations of formalin into awake and anesthetized rats and investigated their behavior and the c-fos immunoreactivity of their spinal cords. METHODS: Sixty adult male Sprague-Dawley rats were divided into 10 experimental groups. Each rat was either manually restrained or subdued through several minutes of 2% to 2.5% halothane inhalation, and then 1 of 5 concentrations of 0%, 5%, 10%, 27%, or 100% formalin (0%, 1.85%, 3.7%, 10%, or 37% formaldehyde solutions) was injected into its rear paw. Nociceptive behaviors were checked for 1 hour, 6 times for each 5-minute period. Two hours after the formalin injections, the rats were killed and c-fos immunoreactivity was measured. RESULTS: Typical responses were observed in the 5% and 10% formalin (1.85% and 3.7% formaldehyde) groups, and nociceptive behaviors were lower in the 27% and 100% formalin (10% and 37% formaldehyde) groups. The number of c-fos-positive cells increased as the formalin concentration increased. Halothane inhalation affected the results of both the behavior and the c-fos immunoreactivity, especially in the 10% formalin (3.7% formaldehyde) group. CONCLUSIONS: It is desirable to describe both formalin and formaldehyde concentrations simultaneously and to do formalin tests without inhalational anesthetics.

Administration, Inhalation↗
PubMedDate not supplied

Visual capability to receive character information. Part I: How many characters can we recognize at a glance?

A study was made on the capability to receive character information and the factors restricting it. The study showed that the capability indicated by the memory span was limited by the average number of characters for words that are made up of individual characters, and calculated in terms of information quantity, there was no difference among the individual characters. The differences in memory span, depending on the size of the pattern presented, was negligible. The difference in the way characters were arranged produced the difference in memory span saturation. This phenomenon is explained by the nature of the lateral interference effect working among the adjacent characters.

Attention↗
PubMedDate not supplied

Visual capability to receive character information. Part II: How long do we need to memorize the random characters briefly presented?

The time-related aspect of the capability to receive character information was measured and analysed. As a result, when the presentation time was shorter than one second, ability was almost constant, but when it exceeded one second it increased as the presentation time increased. It was found that when a pattern was presented without limiting the presentation time, there were linear relationships between the number of characters to be memorized and the time required to memorize all the characters correctly in the range of 2 to 6 characters, 7 to 12 and 13 to 16 (or more). These boundaries corresponded well to a unit of memory well known as the magical number 7 plus minus 2. From these results, it was estimated that a grouping mechanism exists even when memorizing characters and that it has a hierarchical structure.

Attention↗
PubMedDate not supplied

Neutral red assay in minimum fungicidal concentrations of antifungal agents.

We assayed the fungicidal effects of antifungal agents using neutral red staining. Fungal elements of Trichophyton mentagrophytes and T. rubrum were treated with various concentrations of antifungal agents in 96-well filtration plates and then stained with neutral red. The amount of neutral red incorporated by the surviving viable cells was determined from the automated spectrophotometric readings at 550 nm. The minimum fungicidal concentrations (MFCs) of antifungal agents determined by this assay correlated well with those determined by conventional assay. This newly developed procedure should provide a rapid, reproducible, quantitative, qualitative and semi-automated susceptibility test for determination of the MFCs of the fungicidal agents.

Antifungal Agents↗
PubMedDate not supplied

Computed tomography of ovarian masses.

A retrospective analysis of CT images in 138 histologically proven ovarian masses in 100 patients was undertaken to evaluate the usefulness and limitation of CT in the diagnosis of ovarian tumors. Benign masses were purely cystic in 98 (94.2%) and had solid component (including thickened walls, thickened septa, papillary projections) in five of 104 lesions (4.8%) on CT. These five masses, which are classified as benign solid tumors, could not be differentiated from malignant tumors by either the size of the solid portion or the intensity of contrast enhancement. In the malignant tumors a solid portion was detected in 32 of 34 tumors (94%). When a solid component is detected in an ovarian mass, the mass should be considered malignant although a few cases will be benign solid tumors. In Krukenberg tumors, which were all of gastric origin, the solid component was so large that it occupied more than one-half the mass. Therefore, if the solid portion of the ovarian mass is large on CT, upper gastrointestinal study should be performed to rule out Krukenberg tumor.

Diagnosis, Differential↗
PubMedDate not supplied

Facial nerve neuromas: CT findings.

Although neuromas of the facial nerve are rare, they present with uniform clinical and radiological findings. Their pluridirectional tomography findings have been well described; however, the appearance of the intracranial extension of the neuroma which is best visualized by CT has not been emphasized. We report five cases of facial nerve neuromas with particular attention to their intracranial extension. For comparative purposes we also have reviewed 10 cases of acoustic and eight cases of trigeminal neuromas, all involving the cerebellopontine angle (CPA) and the middle cranial fossa. Two of the five facial nerve neuromas affected the second and third segments of the facial canal, and three involved both the CPA and the middle cranial fossa spreading across the midpetrosal bone. This type of tumor extension seems to be characteristic of facial nerve neuromas. In acoustic and trigeminal neuromas the tumor crossing toward the middle fossa takes place via the tentorial hiatus (acoustic) and the petrous apex (trigeminal).

Adult↗
PubMedDate not supplied

Displacement of the quadrigeminal plate in tumors of the fourth ventricle: MR appearance.

Magnetic resonance studies of 12 fourth ventricle tumors were reviewed. The relationship between the tumor and surrounding structures, such as the corpus medullare, the superior medullary velum, and the quadrigeminal plate, was assessed on midsagittal inversion recovery images. Two of the 12 tumors displaced the superior medullary velum superiorly. Seven of the 12 tumors displaced the corpus medullare posteriorly. The superior medullary velum was not recognized in 10 cases and the corpus medullare in 5. Ten of 12 quadrigeminal plates were displaced superoposteriorly due to focal enlargement of the cerebral aqueduct secondary to large intraventricular tumors. In the remaining two cases the tumor was not large enough to enlarge the cerebral aqueduct. The larger the tumor was, the more frequent was the displacement of the quadrigeminal plate. On T2-weighted images, CSF flow-void sign was seen around the tumor in 5 of 12 cases. We consider superoposterior displacement of the quadrigeminal plate to be a relatively constant and reliable sign accompanying fourth ventricle tumors.

Cerebral Aqueduct↗
PubMedDate not supplied

CT diagnosis of catheter-induced septic thrombus of vena cava.

Two cases of septic thrombosis of the vena cava induced by central venous catheterization are reported. Computed tomography showed thrombi containing gas bubbles in the venae cavae; one involving the superior vena cava and the other the left-sided inferior vena cava. Computed tomography also demonstrated surrounding inflammatory infiltrations in the upper mediastinum or retroperitoneum.

Catheterization, Central Venous↗
PubMedDate not supplied

CT of thrombosed renal vein presenting as a hyperdense mass.

We report a patient with focal renal vein thrombosis that presented as a hyperdense mass mimicking a hyperdense cyst or a pelvic or parenchymal tumor. The lesion was described as a well-defined round hyperdense mass in the right renal sinus on noncontrast CT and was surgically proven to be acute thrombosis of the renal vein. Renal vein thrombosis should be considered in the differential diagnosis of a hyperdense mass in the renal sinus even if the patient has no underlying renal abnormality.

Adult↗
PubMedDate not supplied

Intravascular gas within an ovarian tumor: a CT sign of ovarian torsion.

A case of twisted ovarian tumor with intravascular gas is presented, and the mechanism of the gas formation is discussed. On the basis of the probable mechanism of gas formation observed in dead fetuses, the gas seen in the present case is considered to have been oxygen released from trapped oxyhemoglobin within the tumor vessels. "Intravascular gas" within an ovarian tumor, although extremely rare, is diagnostic of ovarian torsion.

Adult↗
PubMedDate not supplied

Gastric cancer: evaluation of triphasic spiral CT and radiologic-pathologic correlation.

PURPOSE: The purpose of this study was to evaluate the utility of triphasic spiral CT with water-filling method in the preoperative T staging of gastric cancer. METHOD: We performed triphasic spiral CT in 108 patients with gastric cancer (53 with early and 55 with advanced gastric cancer). The CT findings were prospectively analyzed and correlated with the histopathologic findings. Spiral CT scans were assessed for the layered pattern of the normal gastric wall, the detectability of tumor, the enhancing pattern of the tumor, and the depth of tumor invasion. RESULTS: The layered pattern of the normal gastric wall was clearly demonstrated in the arterial-dominant or parenchymal phase. All 12 early cancers detected with spiral CT were most clearly depicted in the arterial-dominant or parenchymal phase. On the other hand, 15 (28%) of 54 advanced cancers were most clearly depicted in the equilibrium phase due to the gradual enhancement from the inner mucosal side of the tumor. Most of these tumors were scirrhous type tumor containing abundant fibrous tissue stroma. The accuracy of spiral CT for tumor detection and T staging was 98 and 82%, respectively, in advanced gastric cancer and 23 and 15%, respectively, in early gastric cancer. CONCLUSION: Spiral CT with triphasic scan technique improved the accuracy of estimating the depth of tumor invasion in advanced gastric cancer.

Evaluation Studies as Topic↗
PubMedDate not supplied

CT of neural plexus invasion in common bile duct carcinoma.

PURPOSE: Our purpose was to analyze the CT findings of neural plexus invasion in common bile duct carcinoma. METHOD: We studied 16 patients with common bile duct carcinoma who underwent surgery. Of these, neural invasion was seen in 10 patients. CT findings were retrospectively reviewed and correlated with the surgical and pathological findings. RESULTS: Irregular masses adjacent to the medial aspect of the uncinate process were observed in 4 of 14 patients with distal common bile duct carcinoma. These lesions extended medially and showed contiguity with the superior mesenteric artery and/or celiac axis, corresponding to neural plexus invasion with desmoplastic change. Increased attenuation of the fat between the common bile duct and the proper hepatic artery was seen in two of two patients with proximal common bile duct carcinoma, associated with neural plexus invasion in the hepatoduodenal ligament. CONCLUSION: The location and spread of neural plexus invasion in common bile duct carcinoma are characteristic and can be diagnosed by CT.

Adenocarcinoma↗
PubMedDate not supplied

Renin-producing hepatoblastoma.

Renin-producing tumors of extrarenal origin are rare in children. An 8-year-old boy with hepatoblastoma and hypertension associated with a high plasma renin level is reported. After chemotherapy, the plasma renin level normalized and the hypertension spontaneously resolved. The patient underwent surgery, and a right trisegmentectomy of the liver and a partial resection of the second and third segments were performed. The tumor was as shown the source of renin by immunohistochemical study and reverse transcriptase-polymerase chain reaction.

Child↗
PubMedDate not supplied

Intraneuronal eosinophilic bodies of beef cattle (Japanese brown).

Eosinophilic cytoplasmic inclusion bodies were observed in the midbrain, pons, medulla and spinal cord of beef cattle (Japanese Brown). The inclusion bodies, observed in 14 of 20 cases exhibiting neurological signs and in one of nine healthy cases, differed in morphology and/or staining properties from previously reported inclusion bodies in humans and animals.

Animals↗