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Biomedical subjects

T Fujinaga

Publications and source records attributed to T Fujinaga.

At least 55 records · Page 3Linked to original sources
PubMed1998

Apoptosis induction of POS canine osteosarcoma cells by vitamin D and retinoids.

Vitamin D3: 1-alpha, 25(OH)2D3 (calcitriol), 22-oxa-1,25(OH)2D3 (OCT), cholecalciferol (vitamin D3), and retinoids: all-trans retinoic acid (ATRA) and 9-cis retinoic acid, induced morphological changes in POS canine osteosarcoma cells into elongated, spindle or fibroblast like-shaped cells, and apoptotic like cell death characterized by cell shrinkage, condensation and margination of the nucleus for all drugs at 10(-6)M-10(-9)M after 72 to 120 hr culture. Apoptosis as shown by DNA laddering was induced at 48 hr by all drugs at 10(-6)M, 10(-7)M at 96 hr, 10(-8)M and 10(-9)M at 120 hr respectively. These vitamins are suggested to adjunct antineoplastic agents in canine osteosarcoma therapy by induction of apoptosis.

Animals↗
PubMed1998

Ultrasonographic evaluation of portal vein hemodynamics in experimentally bile duct ligated dogs.

The purpose of this study was to evaluate the relationship between the results of laboratory examinations and ultrasonographic findings, especially portal vein hemodynamics in experimentally bile duct ligated dogs. Biliary obstruction was accomplished by surgically occluding the common bile duct in five dogs. All the dogs became visibly jaundiced within 24 hours after surgery. The total protein and albumin/globulin ratio showed a gradual decrease throughout the examination period, while blood urea nitrogen reached its peak in the 6th week and decreased to pre ligation values by the 10th week. Similar trends were noted in the alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, and direct and total bilirubin. Total cholesterol and fasting serum bile acid levels rapidly increased after surgery to peak values between the 2nd and 4th week, and then gradually decreased, but still remained high throughout the experiment period. The portal flow volume and velocity significantly (p < 0.05) decreased while only a slight increase was noted in the congestion index after bile duct ligation. The cross sectional area of the portal vein changed insignificantly. Bile duct and gallbladder distention was evident within the 1st week after ligation but there was little change in the echogenicity of the liver parenchyma. The results of this study suggest that the determination of Doppler ultrasound parameters of hepatic hemodynamics, especially the portal vein flow indices, may contribute to a better noninvasive assessment of the canine patient with biliary obstructive disease.

Animals↗
PubMed1998

Serum copper and ceruloplasmin activity at the early growing stage in foals.

Serum concentrations of copper (Cu), zinc (Zn), manganese (Mn), calcium (Ca) and inorganic phosphorus (P), as well as antigenic ceruloplasmin (Cp) and oxidase activity as a functional index for copper metabolism, were measured in 10 foals (5 males and 5 females) and their dams. Samples were harvested from the foals within 1 wk after birth and monthly from 1 to 17 mo of age. Samples were collected from their dams in the perinatal period (monthly from 2 mo before delivery to 5 mo postpartum). Serum oxidase activity, antigenic Cp and Cu in foals were extremely low at 1 wk. Serum Cp had the lowest value of 17.0 +/- 8.0 (mean +/- SD) mg/dL within the 1st wk, then increased rapidly up to 43.7 +/- 5.8 mg/dL at 1 mo, and maintained this level until the 17th mo. Serum Zn in foals had the highest value of 73.2 +/- 13.1 micrograms/dL within 1 wk, then decreased to 38.3 +/- 5.9 micrograms/dL by 17 mo. Serum Mn, Ca and P in mares were almost stable and within established reference ranges for our laboratory in the perinatal period, and these values in foals were also in the normal range. Even on appropriate feeding, serum Cu, Cp and oxidase activity were quite low a few weeks after birth, while a higher proportion of Cp-binding copper was found in the foals. This might be caused by the limited synthesis of ceruloplasmin in this period. These data suggest that newborn foals are in a critical situation of marginal copper status in the early stage of growth.

Aging↗
PubMed1998

Establishment of a monoclonal antibody (1/14/16H9) for detection of equine keratan sulfate.

OBJECTIVES: To establish a sensitive and specific monoclonal antibody (MAB) against equine keratan sulfate (KS) and to develop an enzyme immunoassay for measurement of the concentration of KS in serum and synovial fluid from horses. SAMPLE POPULATION: 18 synovial fluid and 48 serum samples were obtained from clinically normal horses and horses with arthritis. PROCEDURE: BALB/c mice were immunized with chondroitinase-ABC-digested proteoglycan monomer from equine joint cartilage, and MAB were raised, using Sp2/O cells as a fusion partner. A competitive ELISA was optimized, using one of the established MAB, and KS concentration in synovial fluid and sera from horses was measured. RESULTS: The high-titer MAB1/14/16H9, which specifically recognizes the epitope on equine KS, was identified. This antibody had no reactivity with chondroitin sulfate and core protein of proteoglycan monomers, hyaluronan, heparin, dermatan sulfate, and heparan sulfate. A competitive ELISA for determination of KS concentration was optimized, using this antibody. Precision data were obtained for the range of 10 to 160 ng/ml. The within- and between-assay coefficients of variation were 10.0 and 12.7%, respectively. CONCLUSIONS: MAB 1/14/16H9 that specifically recognized equine KS was established and was used to develop an enzyme immunoassay for measurement of the concentration of KS in synovial fluid and sera from horses. It is expected that the assay system using MAB 1/14/16H9 will contribute to evaluation of cartilage metabolic activity in horses.

Animals↗
PubMed1997

Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro.

The effects of chitin and its derivatives on the proliferation of fibroblasts and on the production of cytokines were examined in vitro. Chitin and its derivatives showed almost no acceleratory effect on the proliferation of cultured fibroblasts. On the contrary, high-concentration 500 micrograms ml-1) D-glucosamine cultures supplemented with or without a 10% fetal calf serum (FCS) supplementation showed a significant (P < 0.05) reduction in the rate of proliferation of L929 fibroblast cells relative to control. High-concentration chitosan cultures supplemented with 10% FCS showed a significant (P < 0.05) reduction in the rate of L929 fibroblast proliferation. However, the inhibition of cell proliferation by high concentrations of chitosan did not show in cultures without FCS. Interleukin-8 (IL-8) was induced in the supernatants of rat primary cultured dermal fibroblasts stimulated with chitin and its derivatives. Chitin and its derivatives did not stimulate the production of IL-6 by mouse dermal primary cultured fibroblasts. IL-1 alpha, IL-1 beta and tumour necrosis factor-alpha were not detected in the fibroblast supernatants. These observations support the notion that cell proliferation is accelerated indirectly by chitin and its derivatives when these materials are used in vivo. In vivo findings of a angiogenesis and migration of neutrophils may be due to persistent release of IL-8 from fibroblasts.

Animals↗
PubMed1997

Consideration of the optimum pH for the analysis of serum p-phenylenediamine oxidase activity in thoroughbred horses.

The optimum pH for the measurement of serum p-phenylenediamine oxidase (Ox) activity was given (pH 6.6), and the relationship between serum ceruloplasmin (Cp) concentration and its Ox activity was established in healthy adult horses. In adult horses, serum antigenic Cp concentrations were measured by the single radial immunodiffusion (SRID) method with the affinity-purified antibody to equine plasma Cp and compared with its Ox activity. Efficient co-relation between Cp concentration and Ox activity in the sera (r = 0.93) and its Ox/Cp ratio were given. These results might contribute to the calculation of antigenic Cp concentration from its Ox activity, the analysis of holo-Cp content in serum, and the research for copper metabolism in thoroughbred horses.

Animals↗
PubMed1997

Comparison of systemic and renal hemodynamics measured by Doppler ultrasonography in canine experimental hypovolemia.

The aim of this study was to examine renal hemodynamics at the hypovolemic and recovery phases in two different hypovolemic shock models using Doppler ultrasonography, and to compare this with systemic hemodynamics. In experiment 1, the hypovolemic phase was induced in 6 mongrel dogs by removing arterial blood at 30 ml/kg for 60 min. In the recovery phase, this blood was reinfused at 30 ml/kg over 60 min. In experiment 2, hypovolemia was induced in 12 beagle dogs by rapid blood removal until blood pressure decreased to 40 mmHg and was maintained at this pressure for 30 min. Six of the dogs were then infused with 20 ml/kg hydroxyethyl starch over 5 min, and the other 6 were infused with 60 ml/kg lactated Ringer's solution also over 5 min. Parameters for systemic and renal hemodynamics were measured by using a polygraph and the Doppler method, respectively. The decrease of diastolic blood flow, resulted in an increase of vessel resistance, and was detected in the hypovolemic kidney by the Doppler method. The rapid and large volume infusion of resuscitation fluids was effective for the recovery of both systemic circulation and renal blood flow, however this induced an increase of kidney vessel resistance, a result of the autoregulation mechanism of the kidney. The changes in these parameters at the main renal artery and interlobar artery were similar.

Animals↗
PubMed1997

Evaluation of the catabolic activity of cartilage by measurement of serum keratan sulfate concentration in foals.

OBJECTIVES: To determine keratan sulfate (KS) concentration in the serum of foals at the early stage of growing, and to evaluate the role of serum KS as a cartilage catabolic marker, comparing its values with the fluctuation of serum alkaline phosphatase (ALP) activity as a measurement of osteoblastic activity. ANIMALS: 12 foals with normal growth and 3 foals with joint abnormalities within 18 months after birth. PROCEDURE: Measurement of KS concentration and ALP activity in serum and radiographic and physical examinations were done. RESULTS: In all foals, serum KS concentration was high from 1 week after birth to 3 months of age, while serum ALP decreased with aging. The value started to decrease rapidly from 3 to 5 months of age, then gradually reached adult values. During the first 3 months, KS concentration in male foals was significantly higher than that in female foals. In 3 foals which had joint problems, KS concentration was higher than that in normally growing foals at 1 week, and at 1, 2, and 3 months of age. CONCLUSIONS: Cartilage catabolic activity is higher in developing foals up to 3 months of age, suggesting that the immature joint at this time could be easily affected by any factor of loading. Moreover, though only 3 diseased foals were examined, higher serum KS concentration in these foals suggest that this variable might be a useful measure of joint diseases, even at an early stage of life in foals.

Aging↗
PubMed1997

Comparison of regional myocardial technetium-99m-MIBI uptake between ECG-gated and ungated SPECT imaging.

UNLABELLED: Technetium-99m-MIBI uptake was compared between ECG-gated and ungated SPECT images in 10 normal subjects and 10 patients with coronary artery disease to investigate the effects of wall motion on regional myocardial uptake. METHODS: Left ventricular ejection fraction (LVEF) and wall motion were evaluated using the first-pass data acquired immediately after injection of 1110 MBq 99mTc-MIBI. A transaxial ungated image was reconstructed with cumulative data during a cardiac cycle. For transaxial gated images, data during a cardiac cycle were divided into eight frames and the first seven frames were used. The lateral/septal ratios for ungated and gated images were obtained using the counts in the ROIs drawn in the lateral and the septal walls. RESULTS: In 10 normal subjects, the lateral/septal ratio for gated increased during end-systole. The mean of the lateral/septal ratio was significantly lower for ungated than for gated (1.025 vs. 1.077, p < 0.05). In patients with septal wall asynergy and a normokinetic lateral wall, the mean of the lateral/septal ratios was significantly lower for ungated than for gated (1.267 vs. 1.325, p < 0.01). CONCLUSION: Ungated SPECT acquisition may underestimate regional myocardial uptake when myocardial wall motion is good, therefore, ECG-gated data should be acquired for accurate assessment of regional myocardial uptake of 99mTc-MIBI.

Adult↗
PubMed1997

Ultrasonography, biochemical and hematological profiles in liver disease caused by intravenous administration of dimethylnitrosamine in dogs.

The results of liver function tests and ultrasonographical findings were analysed in 7 dogs that were intravenously injected dimethylnitrosamine (DMNA 2 mg/kg body weight) on 2 consecutive days each week for 10 weeks. Typical clinical signs and similar changes in liver enzyme concentrations that develop in dogs with natural cirrhosis were observed in this canine model. Severe anaemia and a significant reduction in the platelet numbers occurred in the dogs that died in the 5th week, while in all the other dogs these parameters decreased slightly. Serum total protein and the albumin/globulin ratio decreased gradually while the alkaline phosphatase, alanine amino transferase, aspartate amino transferase and gamma glutamyl transpeptidase activities increased significantly (p < 0.05) in all dogs after beginning the administration of DMNA. Ultrasound findings of a coarsened and heterogeneous echo pattern with increased echogenicity that are characteristic of canine cirrhosis were noticed at the same time when the changes in liver enzymes became evident. Present results suggest that ultrasonography in conjunction with liver function tests may be useful in the evaluation of experimentally induced liver cirrhosis.

Alanine Transaminase↗
PubMed1997

[Urinary schistosomiasis: report of a case].

A 31-year-old Japanese man visited our department with macroscopic hematuria. He had stayed in Asia, Middle East and Africa during the past several years. He was diagnosed with urinary schistosomiasis because examination of urine and a biopsy specimen of the bladder mucosa revealed Schistosoma haematobium ova. Cystoscopy revealed granulomatous lesions and polypoid patches at the right ureteral orifice, posterior wall and dome of the bladder. Radiological examination showed no obstructive uropathy. Histologically, moderate to severe urothelial dysplasia was found but without evidence of malignancy. The disease has been under good control following treatment with praziquantel. Today international exchange between Japan and other countries is common. Therefore, physicians should consider the possibility of schistosomiasis in patients who have been to endemic areas.

Adult↗
PubMed1996

Augmentative effects of phytohemagglutinin-P on proliferation and cytotoxicity of interleukin-2-activated canine peripheral blood lymphocytes.

Canine peripheral blood lymphocytes (PBL) were simulated with recombinant human interleukin-2 (rhIL-2) alone, or with phytohemagglutinin-P (PHA) and subsequent rhIL-2 in order to obtain large numbers of lymphokine-activated killer (LAK) cells. Incubation of PBL with rhIL-2 alone allowed proliferation of large granular lymphocyte (LGL)-like lymphocytes, and the cytotoxic activity of the lymphocytes against canine transmissible venereal sarcoma cells was detected 5-7 days after the culture onset. However, the lymphocytes died within 2 weeks of culture, resulting in limited generation of functional LAK cells. Thus, PBL pretreated with PHA are subjected to rhIL-2-dependent culture. Small- or middle-sized lymphocytes predominantly proliferated in response to rhIL-2, and proliferation of the lymphocytes was sustained for longer than 30 days by repeated stimulations with PHA and subsequent rhIL-2. Cytotoxicity reached significant levels from 2 weeks after the culture onset and thereafter remained almost constant for at least 2 weeks, leading to large-scale production of the LAK cells. Pretreatment of PBL and PHA seems to enhance the LAK cell functions through modification of the precursors of the effector LAK cells.

Animals↗
PubMed1996

Measurement of extravascular lung water by the double indicator dilution method using heat and sodium in horses under general anesthesia.

Rapid infusion is believed to be harmful to the lung, however, the pathological status of pulmonary edema resulting from excessive fluid therapy in horses has not been clarified because the quantitative diagnosis of pulmonary edema is impossible. To evaluate the precision of the double indicator dilution method using heat and sodium in horses, which allows the quantitative diagnosis of pulmonary edema, we compared extravascular lung water volume measured using a lung water computer based on the theory of the double indicator dilution method with that determined by the direct method. The value of extravascular thermal volume (ETV) determined by the double indicator dilution method was 7.82 +/- 0.62 ml/kg and the detection ratio of ETV to the value of pulmonary extravascular water volume (PEWV) by the direct method was 0.996 +/- 0.038. There was a significant correlation between ETV and PEWV (P < 0.05), and the regression line was Y = 1.23 X - 1.73 with a correlation coefficient of 0.953. The value of extravascular lung water determined by the double indicator dilution method was significantly consistent with that obtained by the direct method, indicating the high precision of the double indicator dilution method in normal horse lungs.

Anesthesia, General↗
PubMed1996

Changes in lymphokine-activated killer activity in peripheral blood lymphocytes from canine transmissible venereal sarcoma models.

Time course changes in anti-tumor activity induced in peripheral blood lymphocytes (PBL) with recombinant human interleukin-2 (rhIL-2) and phytohemagglutinin-P (PHA) were studied in dogs implanted with canine transmissible venereal sarcoma (CTVS) as a tumor-bearing model. The rhIL-2-dependent and PHA-dependent cultures allowed selective proliferation of lymphocytes expressing Thy-1 antigens. The lymphocytes acquired a prolonged anti-tumor activity against the CTVS cells, starting from 2 weeks after the culture, indicating generation of lymphokine-activated killer (LAK) cells. The LAK cells showed serial growth in rhIL-2-containing culture medium for at least a further 2 weeks without loss of the anti-tumor activity.

Animals↗
PubMed1996

Changes of serum cytokine activities and other parameters in dogs with experimentally induced endotoxic shock.

To study the relationship of changes of cytokines in endotoxic shock, serum tumor necrosis factor (TNF), interleukin (IL)-1 and IL-6 like activities, together with physiologic and hemodynamic responses, were examined in dogs before and after intravenous administration of lipopolysaccharide (LPS) purified from Escherichia coli in a dose of 500 micrograms/kg of body weight. The blood endotoxin concentration increased significantly at 30 min after LPS administration, and maintained high levels for 24 hr. Red blood cell counts; hemoglobin concentration and hematocrit values increased at 30 min, and these high values persisted for 24 hr. The platelet count decreased significantly at 30 min, then showed a tendency to recover, but decreased again at 24 hr. Cardiac output, cardiac index and mean arterial pressure showed transient, significant decreases at 15 min, and then returned to the baseline levels by 24 hr. TNF-like activities increased at 30 min, while IL-1-like activities did so between 30 and 60 min. The former reached the maximal levels at 2 hr and the latter at 1.5 hr. Both activities were then hardly detectable from 6 to 24 hr. IL-6-like activities elevated at 1 hr with the peak at 1.5 hr, and remained high until 24 hr.

Animals↗
PubMed1996

[Idiopathic retroperitoneal fibrosis localizing in pelvic cavity: a case report].

We report a case of idiopathic retroperitoneal fibrosis localized in the pelvic cavity. A 54-year-old female was found to have a right hydronephrosis by abdominal ultrasonogram and was admitted to our department. Drip infusion pyelography (DIP) and computed tomography (CT) revealed a right hydroureteronephrosis and a right lower periureteral mass. The mass was confirmed to show low intensity on both T1 and T2 weighted image in magnetic resonance imaging (MRI), which suggested fibrous component. Various tumor marker values were within normal range. Tumor extirpation including lower ureter and ureterovesiconeostomy were performed after confirming pathologically the absence of malignancy by fresh frozen section during surgery. The tumor was diagnosed histologically as idiopathic retroperitoneal fibrosis. We briefly reviewed 223 cases of idiopathic retroperitoneal fibrosis in the Japanese literature.

Female↗
PubMed1996

Ultrasonography and angiographic examination of normal canine liver vessels.

The aim of this study is to provide a description of the ultrasonographic and angiographic anatomy of the hepatic and portal veins in normal dogs. Knowledge of this anatomy is essential for the proper diagnosis and correction of hepatic vessel anomalies like portal systemic shunts. Cranial mesenteric portography was done in 10 adult mongrel dogs to visualize the portal vein and its branches while the hepatic veins were visualized using the transvenous retrograde angiography method. Longitudinal and transverse ultrasound scans of the liver were done in the same dogs in left and right lateral positions in order to determine the best positions for imaging the different liver vessels. The distribution pattern of the hepatic and portal veins could be demonstrated by both ultrasonography and contrast angiography. Hepatic arteries are not usually visualized ultrasonographically. Ultrasound provided dynamic information that could not be obtained by contrast radiography. Super imposition of all vessels in the angiographic studies made the description of the branches of the liver vessels difficult. The results of the present study suggest that both methods may provide important complementary information in the diagnosis and planning of surgical procedures for the correction of vessel anomalies in the canine liver.

Angiography↗