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Biomedical subjects

T Frey

Publications and source records attributed to T Frey.

50 records · Page 3Linked to original sources
PubMed1977

External diseases of the eye.

The child presenting a red, tearing eye can pose a dilemma for the pediatrician. A good history and a careful systematic examination using good light and magnification should lead the physician to the proper diagnosis. The child with no complaints other than discharge probably has a conjunctivitis, while the child with photophobia and a scratchy sensation should make one search for either a foreign body or a corneal problem. Fluorescein dye is invaluable in outlining a corneal scratch, abrasion, dendritic ulcer, or bacterial ulcer; and the use of smears and cultures can help pinpoint the exact cause of the patient's difficulties and help in choosing the proper medication. The care of the patient with a red eye may be difficult; but when properly done, it can be a source of great satisfaction to both the patient and the physician.

Bacterial Infections↗
PubMed1975

Strain of Escherichia coli with a temperature-sensitive mutation affecting ribosomal ribonucleic acid accumulation.

A mutant of Escherichia coli has been isolated that has a temperature-sensitive mutation that results in specific loss of ribosomal ribonucleic acid (RNA) synthesis and some reduction in messenger RNA synthesis. When the strain was grown in glucose medium at a restrictive temperature, RNA accumulation ceased, but both messenger RNA and protein synthesis continued for an extended time. Because carbon metabolism was slowed drastically when strain AA-157 was placed at the restrictive temperature, this phenotype can be compared with carbon depletion conditions present during diauxic lag. However, the phenotype of mutant AA-157 differs from shift-down conditions in that guanosine-3',5'-tetraphosphate levels are unaffected; therefore, a different site is affected. This mutant strain (AA-157) thus shows many characteristics similar to an aldolase mutant previously reported (Böck and Neidhardt, 1966). However, the mutation occurred in a different position on the E. coli genetic map, and furthermore, aldolase was not temperature sensitive in strain AA-157. In this paper we present a study of macromolecular biosynthesis in this mutant.

Amino Acids↗
PubMedDate not supplied

NMR technique for assessing contributions of heavy and light chains to an antibody combining site.

Nuclear magnetic resonance (NMR) has been used extensively to study the structure of antibody combining sites. In recent studies we have observed the proton resonance spectra of the Fab fragment of a monoclonal anti-spin-label antibody derived from a hybridoma grown on various specifically deuterated amino acids. The broadening of the proton resonance signals by the paramagnetic hapten, together with selective deuteration, has allowed the identification of most of the amino acids in the combining-site region of this antibody and has also provided estimates of distances between amino-acid protons and the unpaired electron. Here we show how recombination of specifically deuterated heavy and light chains permits the assignment of single amino-acid proton resonance signals to either the heavy or light chain. In addition, the spectra of such recombinants demonstrate that their combining-site structures must be almost identical to the native structure.

Animals↗
PubMedDate not supplied

Myelinated nerve fibers, axial myopia, and refractory amblyopia: an organic disease.

Six patients with the syndrome of myelinated nerve fibers, high axial myopia, and refractory amblyopia were studied with particular emphasis on their sensory status. Ocular examination included keratometry, B-scan ultrasonography, measurement of axial lengths, and fundus photography. Sensory testing included visual fields, photostress testing, and neutral density filter visual acuities. Electrophysiological testing was also performed on some patients. Our findings suggest an organic etiology for the poor vision, although all patients had superimposed functional amblyopia as well. We suggest that myelination about the macula is the most likely cause of this poor vision and that the myelination, in some way, may have stimulated the development of the high myopia.

Amblyopia↗
PubMedDate not supplied

Long-term effects of transduodenal sphincterotomy: symptoms and hepatobiliary function.

BACKGROUND/AIMS: Endoscopic sphincterotomy is widely used in treating common bile duct stones, but the long-term effects of destroying the sphincter of Oddi are not known. This study investigated the long-term (15-20 years) effects of transduodenal sphincterotomy on gastrointestinal symptoms and hepatobiliary function in a retrospective controlled study. METHODOLOGY: Between 1974 and 1977, choledocholithotomy was performed in 131 patients either transduodenally (60 patients) or via choledochotomy (71 patients). For the retrospective part of the study, all hospital records, death certificates and autopsy reports were reviewed. Twenty-seven patients who could be reached and volunteered to participate (12 and 15, respectively) were re-examined. In the re-examination part of the study, a standard questionnaire interview, laboratory tests, quantitative cholescintigraphy and ultrasonography were performed. RESULTS: The hospital mortality for the primary operations was 0.8%, 0% for the transduodenal sphinterotomies and 2% for the choledochotomies; the difference was not significant. During the follow-up period, no significant differences could be seen in the death rate or in the causes of death between the study groups. During re-examination, flatulence was found to be more common in the choledochotomy subgroup (7/15 vs 2/12, p<0.05). In the laboratory tests, conjugated bilirubin and serum aspartate amino transferase levels were higher in the transduodenal subgroup than in the choledochotomy subgroup (3.3 umol/L, SD 0.4 vs 2.2 umol/L, SD 0.2, p<0.02; and 25 U/L, SD 12.6 vs 18.6 U/L, SD 5.0, p<0.05, respectively). Furthermore, 6/12 (50%) of the transduodenal subgroup had elevated serum alanine aminotransferase, aspartate aminotransferase or amylase levels, as compared to 1/15 (7%) in the choledochotomy subgroup (p<0.02). In terms of ultrasonography, in the choledochotomy subgroup, the common bile duct diameter was larger than 8 mm in 8/15 (53%) patients, as compared to 1/12 (8%) in the transduodenal subgroup (p<0.02). In terms of quantitative cholescintigraphy, in 9/12 (75%) patients of the transduodenal subgroup, the hilum-duodenum transit time was less than 10 minutes, as compared to 4/12 (47%) of the choledochotomy subgroup. CONCLUSIONS: Transduodenal sphincterotomy results in decreased flatulence and enhanced common bile duct drainage, even in the long-term period, but slightly higher serum liver funtion tests, when compared to supraduodenal choledochotomy.

Adolescent↗