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Biomedical subjects

T Finlay

Publications and source records attributed to T Finlay.

9 recordsLinked to original sources
PubMed2000

Expression of angiopoietin-2 by human endometrial endothelial cells: regulation by hypoxia and inflammation.

The functional endometrial layer receives the implanting blastocyst, but is sloughed off during menstruation. Angiogenesis regulates growth and repair of cycling human endometrium. While vascular endothelial growth factor initiates angiogenesis, the angiopoietins (Angs) acting via the Tie2 receptor, are key regulators of subsequent angiogenic steps. This study is the first to localize Ang-2 and Tie2 in human endometrium and to study Ang-2 regulation in cultured human endometrial endothelial cells (HEECs). Immunohistochemistry revealed that expression of Ang-2 and Tie2 was absent from the glands, low in stromal cells, and intense in the endothelial cells. In contrast, only weak expression of Ang-1 was detected. The phase of the menstrual cycle did not appear to affect the expression of Ang-2 or Tie2. In vitro studies were carried out utilizing isolated HEECs, the most relevant model for endometrial microvascular biology studies. Both hypoxia and phorbol-myristate-acetate enhanced Ang-2 mRNA levels in HEECs. These results suggest that Ang-2 plays a role in endometrial pathologies complicated by impaired blood flow and inflammation.

Angiopoietin-1↗
PubMed1992

Use of esterase inhibitors and zone electrophoresis to define bacterial esterases in amniotic fluid.

OBJECTIVE: The purpose of our study was to define further the role of bacterial esterases in amniotic fluid obtained from women with chorioamnionitis. STUDY DESIGN: Amniotic fluid samples from 39 patients with chorioamnionitis were submitted for bacterial cultures and in vitro assay. Esterase inhibitors diisopropyl fluorophosphate and iodoacetic acid were added and the degree of inhibition calculated. These results were compared with the amniotic fluid culture results. Chi square analysis was performed to compare the results of the esterase assay and the inhibition assay between the uninfected and infected amniotic fluid samples. RESULTS: Thirty-one patients had positive bacterial cultures, with 21 being infected with gram-negative organisms. All samples showed significant inhibition (range 55% to 82%) with diisopropyl fluorophosphate. There was partial inhibition with iodoacetic acid (range 10% to 30%) in the gram-negative samples but no inhibition in the gram-positive and uninfected samples. Six infected and two uninfected samples were analyzed by using zone electrophoresis with human plasma as a control. Minimal esterase motility was noted in the amniotic fluid samples as compared with that in plasma. CONCLUSION: The esterases in amniotic fluid appeared to be of bacterial, not human, origin. Furthermore, different groups of bacteria appeared to produce different esterases in infected amniotic fluid.

Amniotic Fluid↗
PubMedDate not supplied

Making sense of the care of patients with pancreatitis.

Pancreatitis is a painful condition with potentially long-term after-effects. This paper describes the condition, its causes and the factors that need to be taken into account both in its immediate management and in terms of its impact on a person's lifestyle.

Acute Disease↗
PubMedDate not supplied

Making sense of bowel preparation.

Investigation and surgery of the gastrointestinal tract requires careful preparation to ensure success of the procedure and a recovery that is free from complications. Different parts of the tract require different preparation. This article outlines the principles of bowel preparation and examines some of the approaches and agents used.

Colonic Diseases↗
PubMedDate not supplied

Making sense of ... parenteral nutrition in adult patients.

Parenteral nutrition is used to support people who have problems eating and drinking or who cannot absorb nutrients through their gut. This paper outlines the rationale for employing parenteral nutrition, the constituents of the solutions used and issues arising from the administration of the therapy.

Adult↗