Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
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Biomedical subjects
Publications and source records attributed to T E Feltkamp.
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The antiperinuclear factor (APF) test is as sensitive and specific for rheumatoid arthritis as the classical rheumatoid factor tests. Five European laboratories decided to perform a consensus study on the interlaboratory variability of the APF test. They used different donor cells, different conjugates and different criteria for positivity. However, the results differed by not more than one two-fold dilution step, when expressed in international units using WHO reference rheumatoid arthritis serum as the standard.