Biomedical subjects

T Dyrberg

Publications and source records attributed to T Dyrberg.

75 records · Page 5Linked to original sources

PubMed1982

Islet cell surface antibodies in spontaneously diabetic BB rats.

Antibodies to normal Wistar rat islet cells are often found in newly diagnosed diabetic BB rats. It was tested if antibodies in BB rats bind to cells from a cloned insulin-producing, rat-insulinoma cell line, RIN 5F, using a protein A-radioligand assay for islet cell surface antibodies. A close correlation was found between the binding of antibodies to islet cells and to RIN 5F cells. Islet cell surface antibodies reactive with RIN 5 F cells were determined in two litters of BB rats, born by diabetic, islet cell surface antibody positive parents. A high incidence (85%) of diabetes was found among 13 offsprings, the mean age of onset being 88 days. At 44 days of age before diabetes was diagnosed, 95% of 20 offsprings were islet cell surface antibody positive. There was no difference in antibody-levels between diabetic and non-diabetic rats. It is concluded that BB rats have circulating antibodies reactive with surface determinants on the RIN-5F insulin-producing cell line. There was a high prevalence of islet cell surface antibodies among young, yet unaffected BB rats.

Animals↗

PubMed1981

Prevalence of diabetic autonomic neuropathy measured by simple bedside tests.

To investigate the prevalence of diabetic autonomic neuropathy, five simple bedside tests, beat-to-beat variation during quiet respiration, beat-to-beat variation during forced respiration, heart rate and blood pressure response to standing, heart rate response to exercise, and heart rate response to Valsalva's manoeuvre were applied to 75 male insulin-dependent diabetics, mean age 40 years, (range 30-49 years). The subjects were subdivided into three groups according to duration of diabetes, which was between 0 and 40 years. Twenty-eight healthy age-matched male controls were also studied. The prevalence of diabetic autonomic neuropathy in the whole diabetic population indicated by abnormal response in beat-to-beat variation during forced respiration was 27%. Diabetic autonimic neuropathy increased in frequency with duration of disease. Patients with nephropathy or proliferative retinopathy had a significantly higher prevalence of diabetic autonomic neuropathy as indicated by abnormal beat-to-beat variation during forced respirations (p less than 0.01) than patients without these complications.

Adult↗

PubMed1981

Islet transplantation in mice differing in the I and S subregions of the H-2 complex.

Diabetic A.TL and A.TH mice, congenic inbred strains differing only in the I and S (non-K, non-D) regions of the major histocompatibility (H-2) complex, received intrasplenic allogeneic and isogeneic pancreatic islet transplants. The isografts were uniformly accepted, while approximately 60% of the recipients receiving allografts rejected the islets, most within 4 weeks of transplantation. With either strain the animals accepting the allografts had higher blood glucose values than normal mice or isografted recipients. After a minimum of 13 weeks of nearly normal glucose levels, successfully allografted mice received skin grafts and spleen cells. None of the A.TH recipients rejected the A.TL islets, but two of four A.TL recipients of A.TH islets became diabetic after the skin grafts. These observations suggest a variable response to islet transplanted across this isolated difference of the H-2 complex. Furthermore, allografted islets resisting rejection did not provide normal glucose levels in the recipients.

Animals↗