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Biomedical subjects

T Doyle

Publications and source records attributed to T Doyle.

66 records · Page 4Linked to original sources
PubMed1975

Laboratory and field studies with a bovine ephemeral fever vaccine.

Bovine ephemeral fever (BEF) virus vaccines, prepared from the brains of suckling mice infected with strain 525 BEF virus, were evaluated in housed cattle and in the field. The virus in lyophilised preparations was stable for 6 months at -50 degrees C. Thirty-four calves, 5 to 18 months old, were used in laboratory vaccination trials. An increase in serum neutralising antibody was detected in 13 of 14 calves initially free of serum antibody, and all 13 failed to develop clinical illness following challenge with virulent BEF virus. Vaccination resulted in no detectable serum antibody increase in 4 calves, 5 months old, with pre-existing antibody of presumed maternal origin. Seven animals, 18 months of age with serum antibody presumed due to previous BEF infection, developed increased antibody titres following vaccination. In 3 animals vaccinated but not challenged, vaccine-induced antibodies decreased to low levels over 5 months. In contrast, the antibody titres following infection with virulent virus in 2 calves were maintained over 5 months. Field trials, involving 236 animals initially free of serum antibody, were conducted on 5 properties near Mackay and 4 properties near Brisbane. Most of 164 animals were vaccinated with a single dose of lyophilised vaccine containing aluminium hydroxide adjuvant. Only 4 animals failed to develop serum antibody and no adverse reactions to vaccination were reported. Natural infection with BEF occurred in 4 herds at Mackay and clinically mild BEF occurred in 3 of 109 vaccinated and 3 of 46 control animals. On the basis of measured serum antibody titres it was assumed that 8 of 53 animals receiving full vaccine volume, 20 of 40 animals receiving half vaccine volume and 18 of 40 control animals became infected with BEF virus. Two dairy herds in Brisbane became naturally infected with virulent BEF virus 7 months after vaccination. Clinical BEF was observed in 8 of 11 control animals and in 3 of 26 animals which received 2 doses of vaccine. Two strains of BEF virus were isolated from unvaccinated animals that developed clinically mild BEF in the field. These strains either failed to infect, or produced subclinical or very mild BEF, when inoculated intravenously into susceptible calves. The anitbody response to natural infection with apparently mild viruses was short-lived, similar to that produced by vaccination.

Animals↗
PubMedDate not supplied

Changing care staff approaches to the prevention and management of aggressive behaviour in a residential treatment unit for persons with mental retardation and challenging behaviour.

The impact of a new training procedure aimed at improving staff skills in the preventative and reactive management of severely challenging behaviours was investigated within a six-place residential treatment unit. The results showed that there was some evidence to support the notion that the training reduced the number of behavioural incidents for most residents. The rates of major reactive strategy use (restraint and emergency medication) also declined over time, as did rates of staff and resident injury. Although only a limited number of these changes showed statistically significant correlations with time, it is argued that they were clinically significant when viewed against the complexity of the client group under study.

Activities of Daily Living↗
PubMedDate not supplied

Transesophageal echocardiography in pediatric congenital heart disease.

The uses of transesophageal echocardiography have expanded dramatically over the last decade. With advances in technology, this imaging modality has become readily available for evaluation of the complex pediatric population with congenital heart disease. This article discusses the many uses of transesophageal echocardiography in this population, in the outpatient setting, in the peri-operative period, and in the cardiac catheterization laboratory.

Ambulatory Care↗
PubMedDate not supplied

Brachytherapy for head and neck cancer: a case study.

Brachytherapy currently is being used as a treatment modality for head and neck cancer. A case study is presented to illustrate the treatment and safety procedures required when brachytherapy is delivered. A multidisciplinary approach is essential to ensure that family and staff exposure to radiation is minimized. Nursing care considerations include preventing airway impairment, maintaining hydration, developing alternative communication methods, pain management, and bowel preparation. Through intensive patient, family, and staff education, patients with head and neck cancer can be treated with brachytherapy safely and effectively.

Antineoplastic Combined Chemotherapy Protocols↗
PubMedDate not supplied

Immunohistochemical localization of proliferating cells, basic fibroblast growth factor and Fos protein following iliac artery balloon angioplasty in hypercholesterolemic rabbits.

The restenotic process in iliac arteries of hyperlipidemic rabbits following balloon angioplasty was evaluated in a temporal sequence by monitoring smooth muscle cell (SMC) proliferation (proliferating cell nuclear antigen -PCNA marker), growth factor (basic fibroblast growth factor -bFGF) production, and Fos oncogene protein expression using immunohistochemical methods. The percutaneous transluminal angioplasty was performed in iliac artery of rabbits fed a 1% cholesterol diet, with a balloon catheter inflated from 4 to 8 atm for 2 min under cineangiographic control until an acceptable (at least 50% stenosis reduction) result was obtained. The morphometric analysis indicated that the number of PCNA-positive cells in contralateral control arteries was low, but was increased in balloon-injury. The bFGF-positive labeled cells were localized in the media and in recently-migrated SMCs within the intima. Two days following angioplasty these cells were more abundant and scattered in the entire neo-intima. After 14 days their number was still higher than that of the controls. Cellular Fos protein expression was detected in the intima and, to a lesser extent, in the media 2 hours after angioplasty. These data suggest that early expression of Fos protein and bFGF in iliac arteries of hyperlipidemic rabbits following balloon angioplasty could be involved in development of restenosis in this animal model.

Alkaline Phosphatase↗