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Biomedical subjects

T Doi

Publications and source records attributed to T Doi.

At least 469 records · Page 26Linked to original sources
PubMed1984

Non-IgG1 nature of cutaneous basophil hypersensitivity factor in contact sensitivity. II. Demonstration of antigen-dependent basophil chemotactic activity of cutaneous basophil hypersensitivity factor.

It was recently demonstrated that there was a specific activity to induce basophil-rich skin reaction in the sera of contact-sensitized guinea pigs (CBH factor, CBH-F). In the in vitro chemotactic assay system, CBH-F was shown to have weak basophil chemotactic activity but enhanced its activity in the presence of corresponding antigen(s). Furthermore, basophil chemotaxis in reaction to the antigen(s) was observed when the cells were preincubated with CBH-F. It worked mainly for Percoll-separated bone marrow basophils but not for oil-induced peritoneal macrophages. Immunological analysis revealed that CBH-F was a protein with a small molecular weight (MW 4-6 X 10(4] with an antigen binding site and isoelectric point of between 4.5 and 5.0. It did not show any characteristics of IgG1 or IgG2 on immunoadsorbent column and immunoelectrophoresis. Enzyme treatment with insoluble trypsin eliminated the chemotactic activity but this was not the case with neuraminidase treatment.

Animals↗
PubMed1984

Distribution of IgG subclasses in membranous nephropathy.

The distribution of human IgG subclasses among the glomerular deposits in human membranous nephropathy was examined by immunofluorescence with subclass specific monoclonal antibodies. Large amounts of granular deposits of IgG4 were identified along the capillary loops in all 12 patients, and seven patients had small amounts of IgG1 deposits. Neither IgG2 nor IgG3 deposits were detectable in any of the patients. On the contrary, all four IgG subclasses were detected in membranoproliferative glomerulonephritis and lupus nephritis with a predominance of IgG1 and IgG3. The results indicate that IgG4 is predominant in the glomerular deposits in membranous nephropathy and may play an important role in its pathogenesis.

Complement System Proteins↗
PubMed1984

Correlation of C3d fixing circulating immune complexes with disease activity and clinical parameters in patients with systemic lupus erythematosus.

Using anti-C3d as a solid phase reagent, C3d fixing circulating immune complexes (CIC) were detected in sera from patients with systemic lupus erythematosus (SLE), rheumatoid arthritis, membranous nephropathy and IgA nephropathy. Particularly, sera from SLE showed the highest CIC levels and highest incidence of positivity among these diseases. In the 51 serum samples from 48 patients with SLE we studied, the CIC detected by the anti-C3d assay correlated well (P less than 0.01) with the CIC detected by the solid phase C1q assay, but not with those detected by the conglutinin assay. In addition, the CIC detected by the anti-C3d assay correlated more significantly (P less than 0.001) with disease activity, as well as some clinical parameters (serum anti-dsDNA antibodies, CH50 and C3 levels) than CIC detected by the other two assays of SLE sera. The anti-C3d binding materials were found to be of intermediate (8-19S) and small (7S) sizes in a small number of SLE sera which we analysed.

Antigen-Antibody Complex↗
PubMed1983

Changes of the mean birth weight in frequency tables with various number of classes--by prefecture, health center and city-town-village.

When the mean birth weights (MBW) calculated from frequency tables are compared with each other, the methods for grouping data into class intervals should be taken into consideration. We integrated the lower and/or upper classes of the frequency tables belonging to the type used in the Vital Statistics of Japan with 500 g intervals and 10 classes (less than or equal to 999, 1000-1499,..., 4500-4999, 5000 less than or equal to) into neighboring classes, and observed the differences between both MBW and the standard deviations (SD) calculated from the original standard frequency tables (o method) and those from the above modified ones (a,..., h method) at three community levels, namely, prefectural, health center (HC) and city-town-village level. At the prefectural level, MBW changed from -2 to 3 g through all the modified methods and SD changed most at -5 g in a,..., d method. At the HC level, MBW changed from -3 to -6 g and SD changed most at -8 g in a,..., d method. At the city-town-village level, MBW and SD did not change in over 50% of the communities, but the degrees of change were large in some communities where MBW or SD changed. The changes were larger in the case of integrating the lowest class into a class of less than or equal to 1499 g than that of integrating the highest class into a class of 4500 less than or equal to. Also they were larger in the case of integrating the lower two classes into a class of less than or equal to 1999 g than that of integrating the upper two classes into a class of 4000 g less than or equal to. We concluded that 1) MBW from the frequency tables where the lower classes were integrated should be carefully dealt with, especially at the small community level, but 2) the differences of population means between any method were not statistically significant and 3) if MBW from different frequency tables are compared with each other, reasonable differences of MBW depending on the methods of grouping and the size of the number of live births should be taken into account.

Birth Weight↗
PubMed1983

Theoretical expression of the mean birth weight and the standard deviation in frequency tables with various number of classes.

Using the birth weight frequency table with 500 g intervals and 10 classes (less than or equal to 999, 1000-1499,..., 4500-4999, 5000 less than or equal to) as a standard table (0 method) and creating 8 kinds of modified frequency tables (a,..., h method) by integrating the lower and/or upper classes in the standard table into neighboring classes, we assessed the differences of both the mean birth weights (MBW) and the standard deviations (SD) between 0 method and 8 kinds of methods (a,..., h method) from the aspect of frequencies belonging to the integrated classes. The equations expressing the differences of MBW were obtained as a function of relative frequencies belonging to the integrated classes. Although the differences of the variances can be exactly expressed as a function of three kinds of variates, namely the relative frequencies, the number of live births and MBW by the corresponding modified method (or 0 method), the differences of SD can be obtained only as approximations of the above function by adding SD by the corresponding modified method (or 0 method). Examining the degrees of approximations at the prefectural, the health center and the city-town-village levels, we were able to recognize good approximation by simple forms with the relative frequencies and SD by the corresponding modified method (or 0 method). We pointed out that these formulas were useful for transforming MBW and SD by 0 method into those by the modified methods and for assessing the differences of MBW and SD.

Birth Weight↗
PubMed1983

Some factors influencing readings of the birth weight.

For the purpose of assessing the accuracy of the reading of birth weight, delivery records of normal live births in O-public hospital (6627 cases from 1966 to 1975) and in A-private clinic (9080 cases from 1955 to 1974) are used. In distributions of the lowest digit, the lower two digits and the lower three digits of the birth weights noted in the records were analysed. Scale used in O-hospital is a counter scale (capacity of 10 kg, minimum graduation of 1 g) and that used in A-clinic is a slanted dial spring scale (capacity of 10 kg, minimum graduation of 50 g). In both establishments the same scales were used consistently. In O-hospital the values having [0] or [5] at the lowest digit were 71.4%. In A-clinic the values having [00] or [50] at the lower two digits were 93.9%. In both establishments the rounding of the reading value were evidently recognized. When the differences of roundings by sex, parity, weight grade and years were tested, only in O-hospital did they gradually tend to increase significantly by years. The reading seems to be influenced by type, capacity and minimum graduation of a scale and criteria for reading the weight. The following were discussed: the reading of the weight of neonate was significant from the point of view of community health and the technical development of the scale appropriate for that purpose was important.

Birth Weight↗
PubMed1983

Modification of the anticodon triplet of E.coli tRNAMetf by replacement with trimers complementary to non-sense codons UAG and UAA.

E. coli tRNAMetf was hydrolyzed with RNase A using a limited amount of the enzyme to give two half molecules lacking the anticodon trimer and 3'-terminal dimer. Chemically synthesized trimers CUAp and UUAp were joined to the 5'-half molecules by phosphorylation with polynucleotide kinase plus ATP followed by treatment with RNA ligase. These modified tRNAMetf species had anticodons complementary to the termination codons UAG and UAA. Two half fragments were joined by a similar procedure to yield a molecule lacking the anticodon trimer and the 3'-dimer. Methionine acceptor activity of these tRNA was tested under conditions in which the CAU inserted control tRNAMetf accepted methionine. It was found that all three modified molecules were not recognized by the methionyl-tRNA synthetase from E.coli. The other sixteen amino acids were not incorporated with partially purified aminoacyl-tRNA synthetases.

Codon↗
PubMed1983

[Medical man-power and community health care in the city of Kitakyushu. Physicians working in the social welfare institutions and administrations].

In 1982, there are 196 physicians who belong to the Kitakyushu Medical Association, working (full time or part time) in 244 social welfare institutions and administrations. Several characteristics regarding their sexes, ages and main specialties were classified by district (Moji, Kokura, Wakamatsu, Yahata and Tobata) and by medical group (class A members: physicians paying full dues including those being employed--mainly in private practice, and class B members: physicians being employed--mainly in public, university or private hospitals). There were 24 members in Moji, 78 in Kokura, 20 in Wakamatsu, 55 in Yahata and 19 in Tobata; 173 members in class A (165 males and 8 females) and 23 in class B (all were males). Among their main specialties, internal medicine was the highest (121 members) followed by pediatrics (31), surgery (20), obstetrics and gynecology (9), orthopedics (6), psychiatry (6), otorhinolaryngology (3) and ophthalmology (1). 32.5 percent (113 members) of all specialists of internal medicine in class A and 47.5 percent (28 members) of all pediatricians in class A work in social welfare institutions and administrations. Among orthopedists or specialists of obstetrics and gynecology class B members were more than class A members. It was noted that the main specialties of 64.3 percent of 126 contract physicians in 158 Day Nurseries were internal medicine (81 members), but on the other hand specialists were needed in some specialized institutions and administrations.

Age Factors↗
PubMed1983

Effects of intrathecally administered pentobarbital and naloxone on the activity evoked in ascending axons of the rat spinal cord by stimulation of afferent A and C fibres. Further evidence for a tonic endorphinergic inhibition in nociception.

The effects of intrathecally administered pentobarbital and naloxone on activity in ascending axons were determined in decerebrate rats with the spinal cord transected at the lower thoracic level. Activity in ascending axons of the spinal cord was recorded below the site of transection and evoked by electrical stimulation of afferent A beta, A delta or C fibres in the sural nerve. Pentobarbital 250 micrograms depressed activity evoked by stimulation of non-nociceptive A beta and nociceptive C fibres; it did not change activity in response to stimulation of A delta fibres. A low dose (100 micrograms) had no effect of A beta and C fibre-evoked activity but depressed spontaneous activity in the ascending axons. Naloxone 5 micrograms enhanced the spontaneous and evoked activities only in those ascending axons which responded to C fibre stimulation. Pretreatment with pentobarbital 250 micrograms prevented the facilitation by naloxone of C fibre-evoked activity. Naloxone was ineffective even when it was administered in a dose of 100 micrograms simultaneously with pentobarbital. Intrathecal injections of magnesium chloride depressed spontaneous and C fibre-evoked activities and markedly reduced the facilitatory effect of naloxone. It is concluded that nociceptive C fibre-evoked activity is subject to the inhibitory control of endorphinergic neurones and that naloxone facilitates this activity by producing release from inhibition.

Afferent Pathways↗
PubMed1983

A comparative immunologic study of IgA nephropathy.

A conglutinin binding assay has been used to detect circulating immune complexes (CIC) containing IgA, IgG, or IgM in sera from patients with IgA nephropathy. IgA class CIC were detected in 40.7% of patient. IgG class CIC were detected only in patients with glomercular IgG deposits. IgM class CIC were detected more often in patients with glomerular IgM deposits than in patients without glomerular IgM deposits. These results demonstrate an association between the immunoglobulin in CIC and those in glomerular deposits. CIC were not detected in sera from most patients with IgA nephropathy by a Clq binding assay, however, since this assay does not detect IgA class CIC. Immunoelectronmicroscopic studies of IgA nephropathy have shown that C3 deposits are localized to the same areas as IgA deposits. In conclusion, we suggest that mesangial IgA deposits are composed of immune complexes and may be derived from CIC.

Antigen-Antibody Complex↗
PubMed1983

An overlapping syndrome of IgA nephropathy and membranous nephropathy?

This is the first report of primary glomerular disease with both mesangial IgA and subepithelial IgG deposits in the glomeruli at the same time. This nephropathy, discovered in 3 patients, is either a new disease entity or an overlapping of IgA nephropathy and membranous nephropathy. Follow-up studies may clarify the pathogenesis of IgA nephropathy and/or membranous nephropathy. In 1 patient the clinical findings resembled those of IgA nephropathy, and in the other 2 they were those of membranous nephropathy. Light microscopy showed generalized diffuse increases in mesangial cells and matrix, and there was slight capillary wall thickening. In the glomeruli, immunofluorescence microscopy demonstrated both granular deposits of IgA in the mesangium and granular deposits of IgG along the capillary loops. On electron microscopy, electron-dense deposits were identified not only in the mesangium but also on the epithelial side of the glomerular basement membrane. These findings were confirmed by the immunoperoxidase technique in electron-microscopic studies of these antibody classes. These glomeruli contained both the dense reaction products of IgA deposits in the paramesangium and mesangial matrix and the dense reaction products of IgG deposits on the epithelial side of the basement membrane.

Adult↗
PubMed1982

Intrathecal pentobarbital prevents naloxone-induced facilitation of the tail-flick response in the rat.

In rats with acute transection of the lower thoracic spinal cord, intrathecal administration of naloxone in doses of 1, 2, 5 and 50 microgram dose-dependently reduced the latency of the tail-flick response to radiant heat. An intrathecal injection of pentobarbital (100 microgram) prolonged the latency and prevented the facilitatory effect of naloxone. It is concluded that the antagonism between naloxone and pentobarbital is of the non-competitive or functional (physiological) type.

Animals↗