Biomedical subjects
Publications and source records attributed to T Dobosz.
64 sperm samples are tested for the evidence of Sperm Acid Phosphatase by agarose isoelectrofocusing with modified blotting. Near pI = 5.0, we observed polymorphic fractions of Sperm Acid Phosphatase. The investigated polymorphism is alike the original one from prostata. Polymorphic patterns are stable in sperm stains up to 6 weeks.
In a sample of 7467 inhabitants of Wrocław and Lower Silesia, the frequencies of ACP alleles were determined as ACPa = 0.3466. ACPb = 0.5684, ACPc = 0.0848, ACPr = 0.001. The above frequencies are similar to those described in other population samples. Phenotypes with rare gene ACPe were described in Poland for the first time. Utility of ACP phenotyping in criminalistical investigation of legal instruments is strongly limited by low stability of enzyme and change of electrophoretic image.
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The red cell GLO phenotypes were determined in two Polish population samples. A total of 1310 people from the region of Lublin (Southeastern Poland, n = 797) and Wrocław (Southwestern Poland, n = 513) were investigated. The gene frequencies were calculated for GLO1 (= 0.4427) and GLO2 (= 0.5573). The evaluation of 372 mother-child pairs showed no deviation from a hereditary hypothesis.
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A good separation of PGM1 isoenzymes from bloodstains has been obtained with an adapted Sonneborn method of electrophoresis on cellulose acetate. This method requires very small quantities of the examined material, a short time of electrophoresis, and is cheaper and simpler than hitherto described. A correct determination of PGM1 phenotypes from bloodstains was easy up to 24 weeks. Occasionally it was possible to determine phenotypes in bloodstains as old as 47 weeks, and in one case even in bloodstains 126 weeks old. This method may be useful in the forensic serology for determination of PGM1 phenotypes from bloodstains.
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In a sample of the Polish population numbering 703 subjects, three GPT types were encountered with the following frequencies: GPT1-1 0.265, GPT 2-1 0,509 and GPT 2-20.226. Frequencies of GPT1 and GPT2 genes were 0.519 and 0.481 respectively. Distribution of types in 217 mother-child pairs and in 12 families with 36 children was consistent with the hypothesis that the GPT system is dependent on a pair of codominant alleles and confirms that the GPT 1-1 and GPT 2-2 types are homozygotes, and GPT 2-1 is a heterozygote.
Phenotypes of the ADA system were determined in a population sample of n = 208. Two ADA phenotypes were encountered: ADA 1-1 with a frequency of 0-82, and ADA 2-1 with a frequency of 0-18. Gene frequencies were: ADA1 = 0-911 and ADA2 = 0-089. Distribution of phenotypes in 9 families with 28 children and in 93 mother-child pairs was consistent with the hypothesis of heredity of the ADA group system.
Types of the AK groups system were determined in a sample of the Polish population numbering 660 subjects. Two phenotypes were found: AK 1-1 with a frequency of 0-933, and AK 2-1 with a frequency of 0-067. Gene frequencies were AK1 = 0-967 and AK2 = 0-033. Distribution of phenotypes in 62 families with 195 children was consistent with the hypothesis according to which heredity of AK types depends on two codominant alleles.
The research was to determine a simple method of phosphoglucomutase phenotype identification in hair bulb. The agarose technique and electrophoresis on cellulose-acetate foil methods were chosen because a small quantity of the maternal available for examination. It was found out that 1 or 2 bulbs are sufficient to identify the PGM1 features if the electrophoresis method is applied and if more bulbs are available, the PGM3 characteristic can also be identified. The modified technique was used for staining the phosphoglucomutase phenotypes.