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Biomedical subjects

T Deguchi

Publications and source records attributed to T Deguchi.

At least 145 records · Page 8Linked to original sources
PubMed1996

[Kinetics of peripheral blood CD34-positive cells and optimal timing of blood stem cell harvest after urological anticancer chemotherapy].

PURPOSE: We evaluated the changes in stem cells in peripheral blood and the optimal timing for harvesting peripheral blood stem cells (PBSCs) following the administration of conventional cancer chemotherapy in patients with urological malignancies. PATIENTS AND METHODS: We evaluated the changes in PBSCs after the administration of cancer chemotherapy in 12 patients with urological malignancies (6 testis cancer, 3 bladder cancer, 3 prostate cancer) using flowcytometry analysis of CD34, a marker of blood stem cell. We evaluated the optimal timing for PBSCs harvesting following the administration of conventional cancer chemotherapy in 6 patients with urological malignancies (2 patients with testis cancer, 3 patients with prostate cancer, 1 patient with bladder cancer) using flowcytometry analysis of CD34 and granulocyte-macrophage colony-forming units (CFU-GM) counts. PBSC samples from 2 patients with prostate cancer were analyzed by the reverse transcriptase-polymerase chain reaction (RT-PCR) method to detect prostate cancer cells. RESULTS: The percentages of CD34-positive cells tended to increase in the peripheral blood during the period of convalescence that followed the induction of marked myelosuppression produced by the cancer chemotherapy. The peak in CD34-positive cells occurred between days 15 and 31 after beginning of cancer chemotherapy. The percentage of CD34-positive cells was increased in the mononuclear cells in harvested PBSCs. The percentages of CD34-positive cells in peripheral blood and in mononuclear cells harvested from PBSCs was correlated with the CFU-GM counts in harvested PBSCs. The CFU-GM counts peaked between days 16 to 31 following cancer chemotherapy. RT-PCR detected prostate-specific antigen (PSA) mRNA expression in PBSCs obtained from 1 of the 2 patients with prostate cancer. An amount of contamination with PSA mRNA reduced as chemotherapy was repeated. CONCLUSION: A sufficient number of PBSCs can be harvested after conventional cancer chemotherapy without the need for special cytotoxic mobilization that may be delayed the patient's treatment. RT-PCR results suggested that the harvested PBSCs should be examined for tumor cell contamination in patients with metastasis in bone marrow. If a sufficient amount of PBSCs will be harvested, the PBSC samples from the patient with bone marrow metastasis after repeated chemotherapy should be used by priority for PBSC transplantation as of today.

Adolescent↗
PubMed1996

[Leiomyosarcoma of the scrotum: a case report].

A case of leiomyosarcoma of the scrotum is reported. A 44-year-old man referred to our hospital with the complaint of swelling of the left scrotal contents. The lesion was about 3 cm in diameter, isolated from testis, epididymis and spermatic cord. Ultrasonography revealed hypoechoic and slightly heterogenic area in the lesion. Surgical resection was easily done. Histological examination showed leiomyosarcoma. Adjuvant therapy was not performed. Three years after the resection, he is alive without any sign of recurrence or metastasis.

Adult↗
PubMed1996

[Effects of amosulalol hydrochloride on intraocular pressure and aqueous humor dynamics in the rabbit eye].

The effects of amosulalol, which blocks alpha 1 adrenoceptors selectively and beta receptors nonselectively almost to the same extent, on intraocular pressure (IOP) and aqueous humor dynamics were studied in pigmented rabbits. Administration of topical amosulalol (0.5%) resulted in a significant difference in IOP between the treated and the contralateral eyes from 0.5 to 6 hours, and the maximum IOP reduction, 6.0 +/- 0.4 mmHg (mean +/- standard error), was observed at 2 hours after administration. Aqueous humor dynamics measurements (mean +/- standard error) in the amosulalol (0.5%)-treated and the contralateral eyes revealed that total outflow facility determined by the two-level constant pressure perfusion method was not significantly different, 0.14 +/- 0.01 and 0.12 +/- 0.01 microliter/min/mmHg, respectively; the aqueous flow measurements determined by fluorophotometry were 3.0 +/- 0.1 and 3.4 +/- 0.2 microliters/min, respectively (p < 0.05, -11%); the uveoscleral outflow measurements determined by the fluorescein isothiocyanate-dextran perfusion method were 0.53 +/- 0.04 and 0.46 +/- 0.04 microliter/min, respectively (p < 0.05, +15%). In conclusion, amosulalol lowers the IOP by inhibiting aqueous production and increasing uveoscleral outflow.

Adrenergic alpha-Antagonists↗
PubMed1996

[An epidemiological study of malformations in infants delivered from diabetic mothers].

The purpose of this study is to investigate the risks of delivery of malformed infants in diabetic mothers in comparison with those in non-diabetic mothers and to clarify risk factors for malformation due to maternal diabetes. The delivery records available at the Department of Obstetrics and Gynecology, Mie University School of Medicine between 1979 and 1992 showed 103 mothers with diabetes mellitus among 4,353 pregnancies. The incidence of malformation in infants born to diabetic mothers was 9.7%, and it was 2.5% overall. By logistic regression analysis of all mothers in this series, diabetes as well as maternal age, and fetal age were shown to be significant risk factors for malformation. A further logistic model for diabetic mothers revealed that a younger age of the mother at the time of labour, low fetal age, older age at onset of diabetes, previous history of pregnancies, and an Hemoglobin A1 (HbA1) level higher than 9% were independent risk factors, among which HbA1 (>9%) showed the highest odds ratio of 24. Careful management is therefore needed to reduce the risk of malformation in pregnancy of diabetic mothers.

Adult↗
PubMed1996

[Unilateral multicystic dysplastic kidney in an adult: report of a case].

A case of unilateral multicystic dysplastic kidney in a 52-year-old woman is reported. Two abnormal round-like calcifications in left upper quadrant abdomen were found on upper gastrointestinal roentgengraphy for health screening. The computerized tomographic (CT) scan revealed a very small left kidney which was not enhanced and the ring and diffuse calcifications in it. It was not visualized by drip infusion pyelography. Left nephrectomy was performed. The kidney, which was very small, had two cysts the walls of which were calcified. The left ureter and renal vessels were absent. Histological appearance shows typical multicystic dysplastic kidney (MCDK). Literature is reviewed. However, the therapy for MCDK is still controversial.

Calcinosis↗
PubMed1996

[A study of serum protein fraction binding to indocyanine green (ICG) by combined method of immunoelectrophoresis and ICG fundus videosystem].

Binding characteristics of indocyanine green (ICG) to human serum were investigated, with a combination of immunoelectrophoresis and an ICG fundus video system. Serum samples were obtained from three healthy volunteers, 1 minute after intravenous administration of 50 mg/2 ml ICG, and then fractionated immunoelectrophoretically on agarose plates. Electrophoretic patterns on these plates could be observed with an ICG fundus video system as well as an immunoviewer. Using anti-human sera, only one infrared fluorescent line showing the ICG binding immunoprecipitate was recognized near the area of beta fraction, which was also identified by the use of anti-beta-lipoprotein (Lp) antibody. We also studied the affinity of ICG for apolipoproteins (Apo) AI, B, and CIII, which were the main protein components of serum Lps. Electrophoresis showed that ICG bound only to Apo-B, but not to the others. These results indicated that ICG mainly bound to beta-Lp in the blood, and that ICG angiographic patterns were directly reflecting the dynamics of serum Lps, especially for LDL. The high affinity of ICG for only Apo-B could explain the reason why ICG mainly bound to beta-Lp among several serum Lps, because large amounts of Apo-B are included in beta-Lp but a little in other serum Lps.

Apolipoproteins B↗
PubMed1996

[Molecular approach to detection of micrometastatic prostatic cancer cells in the lymph nodes and the bone marrow].

We detected micrometastatic prostate cancer cells in lymph nodes and bone marrow using reverse transcriptase-polymerase chain reaction (RT-PCT) specific for prostate-specific antigen (PSA). RT-PCR revealed PSA mRNA in two lymph nodes obtained from two patients with negative histological and immunohistochemical analyses for lymph node metastases. Of 26 patients with negative bone scan imaging, 7 had PSA mRNA detected in the bone marrow by RT-PCR. The RT-PCR will be a relevant tool to allow a more accurate clinical assessment of lymph node and bone metastases in patients with prostate cancer.

Bone Marrow Neoplasms↗
PubMed1995

Evidence for the lack of hepatic N-acetyltransferase in suncus (Suncus murinus).

The abilities of liver cytosol fractions from the suncus and Sprague-Dawley (SD) rats to N-acetylate aniline, p-aminobenzoic acid, p-aminosalicylic acid and 2-aminofluorene (AF) were compared. The cytosol from rats N-acetylated these substrates at efficient rates, whereas the cytosol from the suncus did not N-acetylate these substrates at detectable rates. When AF was given to the suncus, 2-acetylaminofluorene (AAF), a metabolite of AF formed by N-acetyltransferase (NAT), was not detectable in serum, whereas the metabolite was seen clearly in rats. Northern blot and Southern blot analyses, using cDNAs coding for human NATs as probes, indicated that not only the transcripts but also the genes of the enzymes were undetectable in suncus. These results suggest that the suncus is among the few species known to lack NATs.

2-Acetylaminofluorene↗
PubMed1995

High-performance liquid chromatographic determination of prolylcarboxypeptidase activity in monkey kidney.

A simple HPLC procedure for the determination of prolylcarboxypeptidase activity in monkey kidney was established with Cbz-Pro-Ala used as substrate. Decrease of the substrate and increase of the product were stoichiometrically related to each other. Heat treatment at 60 degrees C freed the enzyme preparation of contaminating activities. Data on substrate specificity and influence of inhibitors suggested this method was sensitive for the determination of prolylcarboxypeptidase without the use of a radioactive substrate.

Animals↗
PubMed1995

Simultaneous expression of human CYP3A7 and N-acetyltransferase in Chinese hamster CHL cells results in high cytotoxicity for carcinogenic heterocyclic amines.

To investigate whether several food-derived heterocyclic amines are activated to genotoxic products in human fetal livers, cell lines stably expressing CYP3A7, a human fetus-specific form of cytochrome P450, NADPH-cytochrome P450 reductase, and human monomorphic or polymorphic N-acetyltransferase (NAT1 or NAT2) were established. The expression of CYP3A7 mRNAs and proteins was determined by RNA blot and immunoblot analyses, respectively. The introduction of CYP3A7 cDNA to CR-68 cells which had been transfected with guinea pig NADPH-cytochrome P450 reductase, NAT1, or NAT2 cDNA resulted in increased sensitivity of the cells to aflatoxin B1 compared to parental cells. The cytotoxicity assay for 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) showed that 7P-145 cells, which expressed the reductase, CYP3A7, and NAT2, were approximately 4-, 30-, and 14-fold more sensitive to respective IQ, MeIQ, and MeIQx than parental CR-68 cells. There were no clear differences in sensitivity to these compounds among CHL, CR-68, and the cells which expressed the reductase and CYP3A7 (7R-54), the reductase and NAT1 (CNM-4), the reductase and NAT2 (CNP-40), and the reductase, NAT1, and CYP3A7 (7M-124). From these results, it was suggested that both CYP3A7 and polymorphic NAT2 are required for mutagenic activation of several heterocyclic amines in human fetal livers.

Amines↗
PubMed1995

Parvalbumin- and calretinin-immunoreactive trigeminal neurons innervating the rat molar tooth pulp.

Calcium-binding proteins and neuropeptides were examined in trigeminal neuronal cell bodies retrogradely labeled with Fast blue (FB) from the maxillary molar tooth pulp of the rat. FB-labeled cells were located in the maxillary division of the trigeminal ganglion. Approximately 30 and 50% of the labeled cells were immunoreactive for parvalbumin and calcitonin gene-related peptide (CGRP), respectively. The coexpression of these substances was observed in 9.5% of FB-labeled cells. On the other hand, 2.4% of FB-labeled cells exhibited calretinin-immunoreactivity (CR-ir) and 20% tachykinin (TK)-ir. The coexpression of CR and TK was observed in 1.9% of FB-labeled cells, i.e., most of CR-ir FB-labeled neurons coexpressed TK-ir. An immuno-EM method revealed that all parvalbumin-ir nerve fibers in the root pulp were myelinated and that CGRP-ir nerve fibers were both myelinated (15%) and unmyelinated (85%). The present study indicated that primary nociceptors innervating the rat molar tooth pulp contained parvalbumin and CR and coexpressed these calcium-binding proteins and neuropeptides. It was suggested that peripheral axons of parvalbumin-ir tooth pulp primary neurons are all myelinated. Most peripheral CR-ir axons are probably unmyelinated because TK-ir myelinated axons have never been demonstrated in any peripheral organ.

Animals↗
PubMed1995

Coordinate expression of vesicular acetylcholine transporter and choline acetyltransferase in sympathetic superior cervical neurones.

The neurotransmitter acetylcholine is synthesized by choline acetyltransferase (ChAT) and transported into synaptic vesicles by the vesicular acetylcholine transporter (VAChT). Recently it has been reported that the entire coding region of VAChT mRNA is located in the first intron of the ChAT gene. In this study, ChAT and VAChT mRNAs were analysed in cultured sympathetic neurones. Cholinergic differentiation factor/leukaemia inhibitory factor and ciliary neurotrophic factor induced strong expression of ChAT and VAChT mRNAs in parallel. RT-PCR analysis of ChAT mRNAs revealed that five types of ChAT transcripts which differed in the 5' non coding regions were increased. RT-PCR analysis of VAChT mRNA indicated that the cytokines induced only VAChT mRNA species which did not contain the R-exon, and not those containing the R-exon. The results indicate that ChAT and VAChT expressions are coordinately but differentially regulated in cultured sympathetic neurones.

Acetylcholine↗
PubMed1995

Complementary DNAs for two arylamine N-acetyltransferases with identical 5' non-coding regions from rat pineal gland.

A cDNA library was constructed from the pineal gland of rats injected with isoproterenol and screened with 32P-labeled cDNAs encoding arylamine N-acetyltransferases from rabbit and human liver. Two types of cDNAs for arylamine N-acetyltransferases (A-type and B-type) were isolated. Expression of the cDNAs in Chinese hamster ovary cells indicated that A-type N-acetyltransferase acetylates both arylamines and arylalkylamines, while the B-type enzyme acetylates only arylamines. Therefore, neither the A-type nor the B-type of enzyme seems to be the arylalkylamine N-acetyltransferase involved in melatonin synthesis in the pineal gland. Nucleotide sequence analysis revealed that both A-type and B-type cDNAs code for 290 amino acids, and that they showed 82.8% similarity in the coding region. However, the nucleotide sequence in the 5' non-coding region was identical in the A-type and B-type cDNAs. In addition, the 5' non-coding region contained another possible open reading frame for 79 amino acids. Data base research revealed that the complementary sequence of the 5' non-coding region has high similarity with the coding regions of cDNAs for high-mobility-group proteins (HMG) 1 and 2, which are thought to regulate mRNA transcription.

Amino Acid Sequence↗
PubMed1995

cDNA cloning and chromosomal localization of the human ciliary neurotrophic factor gene.

Full-length cDNA for human ciliary neurotrophic factor (CNTF) was isolated from a human sciatic nerve cDNA library. Sequence analysis revealed that the longest cDNA was comprised of a 48-bp 5'-untranslated region, a 600-bp coding region and a 1207-bp 3'-untranslated region containing four ATTTA pentamer motifs and a polyadenylation sequence. The transcription starting point was assigned at 81 bp upstream of the initiation methionine by 5' RACE analysis. Using the cDNA and genomic DNA fragment including the entire intron region as mixed probes, the human CNTF gene was localized to the long arm of chromosome 11 at region q12 by fluorescence in situ hybridization.

Chromosomes↗
PubMed1995

Expression of c-myc in altered hepatic foci induced in rats by various single doses of diethylnitrosamine and promotion by 0.05% phenobarbital.

Among the proto-oncogenes examined by northern blot analysis, c-myc, c-Ha-ras, c-fos, and c-raf-1 have been reported to be activated in rat liver cell carcinomas. However, there are relatively few reports on protooncogene expression in altered hepatic foci (AHF) early during hepatocarcinogenesis in the rat. In this study, diethylnitrosamine (DEN) at doses ranging from 10 to 200 mg/kg was used to initiate and phenobarbital (0.05%) to promote AHF in rats. AHF were detected by the presence of the marker enzymes glutathione s-transferase, placental form (GST-P); gamma-glutamyltranspeptidase (GGT); glucose-6-phosphatase (G6Pase); and canalicular adenosine triphosphatase (ATPase). Proto-oncogene expression in individual AHF was investigated by in situ hybridization (ISH). ISH for the mRNAs of c-Ha-ras, c-fos, and c-raf-1 revealed little or no expression in AHF. However, the levels of c-myc mRNA were increased in about 10% of the AHF initiated by the highest dose of DEN (200 mg/kg). Thus, altered expression of proto-oncogenes was not seen in AHF initiated by nonnecrogenic doses of DEN and promoted by phenobarbital. However, at the necrogenic dose of 200 mg/kg DEN, c-myc expression was found mostly in AHF in which abnormal expression of GST-P, GGT, G6Pase, and ATPase was also present, indicating that c-myc expression is correlated with phenotypically greater complexity of the AHF, a characteristic of malignant hepatic neoplasms in the rat.

Animals↗
PubMed1995

Expression of estrogen receptor in diseased human prostate assessed by non-radioactive in situ hybridization and immunohistochemistry.

To understand the role of estrogen in the pathogenesis of benign prostatic hyperplasia, expressions of estrogen receptor (ER) mRNA and ER protein by in situ hybridization and by immunohistochemistry, respectively, were investigated in human prostatic tissues. In non-malignant region, ER mRNA and ER protein were found in cytoplasm and nucleus, respectively, of stromal cells, but not in glandular epithelial and basal cells. In benign regions, ER mRNA/ER protein positive cells were found in fibromyoadenomatous and myoadenomatous hyperplasia, but not in adenomatous hyperplasia. A striking feature was periacinar arrangement of ER mRNA/ER protein positive stromal cells in all prostate carcinoma treated with androgen withdrawal. The ER mRNA/ER protein positive cells were immunohistochemically identified as fibroblasts, myoblasts, and smooth muscle cells. These results indicate that stromal cells are the primary target of estrogen in prostate, and that androgen withdrawal upregulates the expression of ER gene.

Aged↗