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Biomedical subjects

T Coleman

Publications and source records attributed to T Coleman.

66 records · Page 4Linked to original sources
PubMed1985

Regulation of methionine synthesis in Escherichia coli: effect of metJ gene product and S-adenosylmethionine on the in vitro expression of the metB, metL and metJ genes.

The regulation of the expression of three Escherichia coli met genes, metB, which codes for cystathionine gamma-synthetase (EC 4.2.99.9), metL, which codes for aspartokinase II-homoserine dehydrogenase II (EC 2.7.2.4-EC 1.1.1.3) and metJ, which codes for the methionine regulon aporepressor, has been studied using highly purified DNA-directed in vitro protein synthesis systems. In a system where the entire gene product is synthesized, the expression of the metB and metL genes is specifically inhibited by MetJ protein (repressor protein) and S-adenosylmethionine (AdoMet). In a simplified system that measures the formation of the first dipeptide of the gene product (fMet-Ala for the metJ gene), MetJ protein and AdoMet partially repress (approximately 40-60%) metJ gene expression. Thus, the metJ gene can be partially autoregulated by its gene product.

Bacterial Proteins↗
PubMed1985

Cloning and expression of the metE gene in Escherichia coli.

A lambda-transducing phage was isolated that contains the metE gene. This gene codes for N5-methyl-H4-folate:homocysteine methyltransferase (EC 2.1.1.14), an enzyme that catalyzes the terminal reaction in methionine biosynthesis. A 9.1-kb EcoR1 fragment of this phage, containing the metE gene, was then cloned into pBR325. This plasmid, pJ19, was used to transform Escherichia coli strain 2276, a metE mutant, and restore the MetE+ phenotype. Although the transformed cells produced large amounts of the metE protein in vivo, in vitro studies using pJ19 as template showed low synthesis of the metE protein.

Bacteriophage lambda↗
PubMed1985

Regulation of methionine synthesis in Escherichia coli: Effect of metJ gene product and S-adenosylmethionine on the expression of the metF gene.

The regulation of the expression of the Escherichia coli metF gene, which codes for 5,10-methylenetet-rahydrofolate reductase (EC 1.1.99.15), has been investigated by using a simplified DNA-directed in vitro system that measures the formation of the first dipeptide (fMet-Ser) of the gene product. The synthesis of fMet-Ser directed by a plasmid containing the metF gene is specifically inhibited by metJ protein (repressor protein). S-Adenosylmethionine enhances the inhibition by the metJ protein of metF gene expression. The inhibition by the metJ protein is at the level of transcription and the results suggest that S-adenosylmethionine is functioning as an allosteric effector.

Journal Article↗
PubMed1983

Implementing an industrial hearing conservation program in the schools.

The harmful effects of exposure to high noise levels have been well-documented and include auditory and non-auditory factors. The most notable auditory effect is permanent loss of hearing, many times accompanied by the constant perception of a ringing-type noise in the ears (tinnitus). Despite this, there has been insufficient attention given to the hazardous effects of noise in the school health education curriculum. The industrial arts program is one area that deserves special attention because of the noise levels that teachers and students are exposed to in the classroom and the assumed future exposure that students will experience in their recreational and work experiences. This report describes a hearing conservation program implemented in the Industrial Arts Program in the Dallas Independent School District that attempts to provide: 1) protection for students and teachers using equipment that produces excessive, damaging noise; and 2) information to students on the hazardous effects of noise, so they will understand the consequences of not protecting their ears when exposed to excessive noise and the available means to protect their hearing. As the cost of the program was minimal, other school districts could implement similar programs with little additional resources and effort.

Adolescent↗
PubMed1983

Future family physicians get early community contact in Morehouse curriculum.

The pre-clinical curriculum of the Morehouse School of Medicine is unique in several ways. One of the ways in which this curriculum is unique is the manner in which it has involved practicing physicians in the education of medical students during their first two years of medical school. Throughout the country, many physicians have expressed an interest in this involvement of community physicians in the teaching of medical students during what might be their most impressionable period of development. The article which follows describes concisely the development of Community Medicine and Family Practice at the Morehouse School of Medicine, highlighting the issues which relate to the role of community physicians in the teaching of medical students during the pre-clinical years. It is the contention of Morehouse officials that the involvement of practicing physicians in the early education of medical students is very important and that it does not necessitate a compromise of the basic science education which is taking place at this time.

Clinical Clerkship↗
PubMed1974

Remission of diabetes mellitus during pregnancy.

Two cases are reported in which a remission of diabetes was sustained throughout pregnancy. This seemed to be due in part to improved beta-cell function consequent on restoration of normoglycaemia before pregnancy, and in part to an increase in insulin sensitivity during pregnancy, which in the first case disappeared very rapidly after delivery.

Adult↗
PubMedDate not supplied

Using content analysis of video-recorded consultations to identify smokers' "readiness" and "resistance" towards stopping smoking.

Although physicians' brief advice against smoking is effective in helping smokers to stop, very little is known about the process of counseling smokers. We describe the development of a coding system for describing smokers' motivation to stop smoking as judged by their behaviour when discussing smoking with their family physician. We analysed video-recordings of consultations between 47 self-reported smokers and 29 family physicians where smoking was discussed. By a process of observing recordings, reading transcripts and discussion, we developed a coding system for describing smokers' levels of motivation to stop smoking. This consists of four behaviours thought to indicate "readiness" (higher motivation to stop) and four "resistance" (lower motivation to stop). Interobserver reliability between two observers was good (Kappa = 0.71 for readiness, 0.73 for resistance behaviours). We discuss the relevance of our descriptions for clinicians and the possibility of developing this work further to enable systematic research into doctor-patient interactions where smokers are counseled to stop.

Attitude to Health↗
PubMedDate not supplied

Untrivial pursuit.

Explore the source record for details and available documents.

Female↗