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Biomedical subjects

T Cole

Publications and source records attributed to T Cole.

At least 109 records · Page 6Linked to original sources

Age, sex, and ischaemic heart disease as prognostic indicators in long-term cardiac pacing.

The influence of age, sex, and overt ischaemic heart disease on survival after pacing was studied in a population of 427 patients. The median survival for the whole group was 7.75 years. Women between 55 and 74 years and men between 65 and 74 years enjoyed median survivals in excess of 10 years. Patients over 74 years and men with ischaemic heart disease had median survivals of the order of 5 years and 3 1/2 years, respectively. The findings may help to match generator lifetimes to patient-survival prospects.

Adolescent↗

Cell lineages of the embryo of the nematode Caenorhabditis elegans.

Embryogenesis of the free-living soil nematode Caenorhabditis elegans produces a juvenile having about 550 cells at hatching. We have determined the lineages of 182 cells by tracing the divisions of individual cells in living embryos. An invariant pattern of cleavage divisions of the egg generates a set of stem cells. These stem cells are the founders of six stem cell lineages. Each lineage has its own clock--i.e., an autonomous rhythm of synchronous cell divisions. The rhythms are maintained in spite of extensive cellular rearrangement. The rate and the orientation of the cell divisions of the cell lineages are essentially invariant among individuals. Thus, the destiny of cells seems to depend primarily on their lineage history. The anterior position of the site of origin of the stem cells in the egg relates to the rate of the cell cycle clock, suggesting intracellular preprogramming of the uncleaved egg. We used a technique that allows normal embryogenesis, from the fertilized egg to hatching, outside the parent under a cover glass. Embryogenesis was followed microscopically with Nomarski interference optics and high-resolution video recording.

Animals↗

Induction of endogenous virus and of thymidine kinase by bromodeoxyuridine in cell cultures transformed by Friend virus.

Thymidine kinase positive (TK(+)) N type cell lines that had been transformed by spleen focus-forming virus were established by transformation with NB tropic Friend virus complex. Thymidine kinase deficient (TK(-)) cell clones were isolated. Some of these cell clones release 1000- to 100,000-fold reduced amounts of Friend virus complex as compared to the TK(+) parental cell clone. TK(-) clones were grown in medium without BrdUrd. Some of these TK(-) clones can be induced to release endogenous helper virus and transforming spleen focus-forming virus on reexposure to 10(-6)-10(-4) M BrdUrd. The induced Friend virus complex is of N host range as expected with induced endogenous virus in N-type cells. Before the induction of the endogenous virus spleen focus-forming virus complex, an induction of thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.75) activity is observed. The latter is possibly a prerequisite for the induction of endogenous virus in TK(-) cells. Induction of thymidine kinase activity and of endogenous virus is transient and always correlated. The role of BrdUrd and another thymidine analogue, azidothymidine, in interfering with C-type virus release in virus positive cells is discussed. Azidothymidine is unable to induce endogenous virus. Induction of endogenous virus by BrdUrd and inhibition of virus release in virus positive cells is apparently not caused by the same mechanism.

Animals↗

Quasi-elastic light scattering by diffusional fluctuations in RNase solutions.

Using measurements of quasi-elastic light scattering spectra, we have investigated diffusional fluctuations of RNase. The diffusion coefficient for individual protein molecules, together with the corresponding calculated effective molecular radius R(eff), were determined. Between room temperature and the point of irreversible denaturation at 63.5 degrees C, R(eff) increased from 20-250 A. This is comparable to the plateau in R(eff) of 300 A reached after about 200 min following chemical denaturation in 10 M urea. The measurements indicated the presence of a large size component even in the freshly prepared and chromatographically purified solutions. From the diffusion constants deduced for this large component we obtained effective sizes from 1000-5000 A. Concentration and temperature dependent measurements exclude the possibility that these large particles are impurities and indicate that they are the result of aggregations of RNase molecules.

Diffusion↗

Studies of Raman spectra of water solutions of adenosine tri-, di-, and monophosphate and some related compounds.

Using a He-Ne CW laser source together with a digital photon counting system, we have obtained well resolved Raman spectra for adenosine mono-, di-, and triphosphate (AMP, ADP, ATP) in aqueous solution. Spectra of these compounds were studied as a function of pH from pH = 0.5 to 13.5 and between 550 and 1700 cm(-1). It was found possible to distinguish spectroscopically between the three phosphates over the pH range studied. A qualitative analysis of vibrational modes responsible for various spectral lines is given. Lines at about 960 and 1100 cm(-1) were found to be good indications of the degree of ionization of the terminal phosphate group.

Adenine Nucleotides↗

Double intussusception fatally complicated by clostridial infection in a dog (a case report).

A 10-month-old Alsation dog with history of anorexia, diarrhoea, dehydration and vomition developed a double intussusception which affected the distal jejunum and proximal ileum. Necropsy revealed the intussusception to be swollen and congested with fibrinous adhesions between the intussusceptum and intussuscepiens. Two pieces of bone believed to be the inciting cause were found within the intussusceptum. Clostridium welchii and Clostridium bifermentans were isolated from the lesions.

Animals↗

The type I and type II 11beta-hydroxysteroid dehydrogenase enzymes.

Local tissue concentrations of glucocorticoids are modulated by the enzyme 11beta-hydroxysteroid dehydrogenase which interconverts cortisol and the inactive glucocorticoid cortisone in man, and corticosterone and 11-dehydrocorticosterone in rodents. The type I isoform (11beta-HSD1) is a bidirectional enzyme but acts predominantly as a oxidoreductase to form the active glucocorticoids cortisol or corticosterone, while the type II enzyme (11beta-HSD2) acts unidirectionally producing inactive 11-keto metabolites. There are no known clinical conditions associated with 11beta-HSD1 deficiency, but gene deletion experiments in the mouse indicate that this enzyme is important both for the maintenance of normal serum glucocorticoid levels, and in the activation of key hepatic gluconeogenic enzymes. Other important sites of action include omental fat, the ovary, brain and vasculature. Congenital defects in the 11beta-HSD2 enzyme have been shown to account for the syndrome of apparent mineralocorticoid excess (AME), a low renin severe form of hypertension resulting from the overstimulation of the non-selective mineralocorticoid receptor by cortisol in the distal tubule of the kidney. Inactivation of the 11beta-HSD2 gene in mice results in a phenotype with similar features to AME. In addition, these mice show high neonatal mortality associated with marked colonic distention, and remarkable hypertrophy and hyperplasia of the distal tubule epithelia. 11Beta-HSD2 also plays an important role in decreasing the exposure of the fetus to the high levels of maternal glucocorticoids. Recent work suggests a role for 11beta-HSD2 in non-mineralocorticoid target tissues where it would modulate glucocorticoid access to the glucocorticoid receptor, in invasive breast cancer and as a mechanism providing ligand for the putative 11-dehydrocorticosterone receptor. While previous homologies between members of the SCAD superfamily have been of the order of 20-30% phylogenetic analysis of a new branch of retinol dehydrogenases indicates identities of > 60% and overlapping substrate specificities. The availability of crystal structures of family members has allowed the mapping of conserved 11beta-HSD domains A-D to a cleft in the protein structure (cofactor binding domain), two parallel beta-sheets, and an alpha-helix (active site), respectively.

11-beta-Hydroxysteroid Dehydrogenases↗

The growth of boys in the Stuttgart Carlschule, 1771-93.

Height measurements taken in a mixed longitudinal manner on 1084 German-born boys aged 7 to 21+ at the Carlschule Academy in Stuttgart during the period 1771-93 have been examined. The boys can be divided into upper (aristocrat), middle and lower (artisan, servant) classes, nearly all housed and fed in this boarding school. Preece-Baines curves have been fitted to a subsample of 155 boys whose measurements cover at least the period 12-16 years at a density of two or three per year. In addition, the whole data, totalling 11,040 observations, have been examined as if purely cross-sectional; and the height-at-entry measurement for each of 670 students has been examined. The results of the longitudinal subsample and the cross-sectional analyses agree reasonably well. Social class differences existed both in tempo of growth as signified by age at peak height velocity, and in adult height. The longitudinal analysis gives adult differences of about 2 cm between upper and middle classes and a further 2 cm between middle and lower, even amongst these boys all resident in the same, very privileged, school. Tempo differences between upper and middle class were minor, amounting to only 0.3 year, but lower-class boys had their maximum growth increment about a year later than the others. Amongst middle classes a secular trend of about 2 cm averaged over all ages was found between those born before 1770 and those born later. This mainly represents a trend in tempo rather than in adult height. The heights of these boys are compared with those of contemporary Austrian upper and lower classes, English upper and lower classes, American Army cadets, and American slaves. The increase in German middle-class heights during the 18th century indicates that this group was improving its nutritional status and well-being, at a time when the heights of the remainder of the population were constant or declining. This is evidence in favour of the view that at the beginning of economic development the distribution of income tends to become more skewed.

Adolescent↗