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Biomedical subjects

T Chang

Publications and source records attributed to T Chang.

At least 199 records · Page 11Linked to original sources
PubMed1987

Electroelution of DNA and protein from polyacrylamide and agarose gels.

An electroelution method is described for the recovery of DNA and protein from agarose or polyacrylamide gels. The samples to be electroeluted are compartmentalized in a modified microcentrifuge tube fitted with dialysis membranes. This procedure is simple, rapid, inexpensive and efficient. Within 30 min to 2 hrs, the recovery of the sample is nearly quantitative. DNA fragments recovered can be directly subjected to DNA sequence analysis or enzymatic reactions after ethanol precipitation. Proteins can also be recovered after separation by acrylamide gel in the presence or absence of detergents and be ready for further analysis.

Animals↗
PubMed1986

Characterization of lens crystallins and their mRNA from the carp lenses.

Crystallins from carp eye lenses have been isolated and characterized by gel permeation chromatography, SDS-gel electrophoresis, immunodiffusion and amino acid analysis. gamma-Crystallin is the most abundant class of crystallins and constitutes over 55% of the total lens cytoplasmic proteins. It is immunologically distinct from the alpha- and beta-crystallins isolated from the same lens and its antiserum shows a very weak cross-reaction to total pig lens antigens. Comparison of the amino acid compositions of carp gamma-crystallin with those of bovine gamma-II, haddock gamma- and squid crystallins indicates that gamma-crystallin from the carp is very closely related to that of the haddock, and probably also related to the invertebrate squid crystallin. In vitro translation of total mRNAs isolated from carp lenses confirms the predominant existence of gamma-crystallin. The genomic characterization of carp crystallin genes should provide some insight into the mechanism of crystallin evolution in general.

Amino Acids↗
PubMed1986

Non-obstructive idiopathic pachymeningitis cervicalis hypertrophica.

Two young men had similar nonobstructive idiopathic pachymeningitis cervicalis hypertrophica, causing chronic (13 and 11 years respectively) C8-T1 radiculomyelopathy proved by surgical and pathological findings. The preoperative Queckenstedt tests and myelography showed no evidence of CSF obstruction. These unusual findings contrast with previous reports which all described complete or at least partial, block. The findings on metrizamide computed tomogram have not been described before. In the two patients it revealed diffuse cord atrophy from C7 to T2 and hemiatrophy with lateral beaking from C4 to C7. The patients benefited from multiple transverse durotomies. The main pathogenesis of the cord atrophy was the compromizing of feeding radicular arteries rather than direct compression.

Adult↗
PubMed1986

Benign hydrocephalus in infants. A computed tomographic and clinical correlative study.

During the past 3 years we studied benign hydrocephalus in 7 infants, who presented symptoms of macrocrania and mild psychomotor retardation. Computed tomography (CT) revealed dilatation of CSF space resembling brain atrophy or subdural effusion. These children were observed clinically and were found gradually to restore to normal with regard to CT changes, as well as clinically. These patients were compared with another group of patients with subdural effusion or hematoma who needed tapping or shunting.

Brain↗