Biomedical subjects

T C Ghosh

Publications and source records attributed to T C Ghosh.

21 records · Page 2Linked to original sources

PubMed1996

GENOLYTE: a PC-based computer program for visualisation of genomic DNA sequences.

This paper describes the program "GENOLYTE" which has been developed for the visualisation and identification of the global and local patterns of long genomic DNA sequences quickly in a few minutes, with the aid of a microcomputer. Apart from global and local identification, GENOLYTE comparatively depicts the similarity of two or more than two genomic DNA sequences. The utility of the program has been demonstrated by analysing the complete mitochondrial DNA sequences (taken from EMBL databank) as an example and the results are discussed from therein. The program written in TURBO C++ has the following minimum requirements: (i) PC 386 AT, (ii) SVGA colour monitor, (iii) the [BGI] directory of TURBO C++ version 3.0.

Animals↗

PubMed1990

Studies on the conformation of and metal ion binding by teichoic acid of Staphylococcus aureus.

Teichoic acid (TA) isolated from the gram-positive bacteria S. aureus binds cationic dyes like pinacyanol (PCYN), 1,9-dimethyl methylene blue, acridine orange, etc., depicting blue-shifted metachromasia, and they bind the cationic dye carbocyanine depicting the red-shifted J band. TAs do not show any uv absorption band, and exhibition of tailing CD in the short uv region hints at its chiral conformation. Chiral conformation of TA has been confirmed from the induction of strong biphasic CD in the TA-carbocyanine system. Relative affinities for Ca2+, Mg2+, and Na+ have been probed from the disruption of metachromasia of the TA-dye system by these ions. Results show Ca2+ and Mg2+ to be almost equally effective in destroying the metachromasia of the TA-PCYN system, thus not supporting the hypothesis of special affinity for Mg2+ ion.

Calcium↗

PubMed1990

Induction of metachromasia and circular dichroism in the dye 1,9-dimethyl methylene blue by S. aureus wall teichoic acid.

Teichoic acid (TA) isolated from the Gram-positive bacteria Staphylococcus aureus induces blue shifted metachromasia in the dye 1,9-dimethyl methyleneblue (DMMB). Results of conductometric titrations show that DMMB binds to TA with 1:1 stoichiometry. Unlike DNA, this TA does not induce a definite metachromatic band in the dye acridine orange. S. aureus TA also induces strong circular dichroism (CD) in this dye with molar ellipticity value of the order of 10(4). This induction of CD in DMMB supports our earlier inference that TA in dilute solution at neutral pH has a helical conformation, and is not a random coil polymer.

Circular Dichroism↗