Search PubMed⌕ Search

Biomedical subjects

T C Aw

Publications and source records attributed to T C Aw.

96 records · Page 6Linked to original sources
PubMedDate not supplied

A preliminary evaluation of serum proteins by capillary electrophoresis.

We evaluated the 5-band Serum Proteins by Capillary Electrophoresis kit (Bio-Rad Laboratories, Hercules, CA, U.S.A.) on the BioFocus 2000 CE (Bio-Rad) against conventional agarose gel electrophoresis (AGE) (Helena Laboratories, Beaumont, TX, U.S.A.). Serum from 60 patients was initially screened by AGE and divided into three groups: 1) normal electrophoretic pattern (n = 36, mean total protein 67.7 g/L), 2) monoclonal/oligoclonal gammopathy (n = 14, mean total protein 78.8 g/L), and 3) polyclonal gammopathy (n = 10, mean total protein 77.4 g/L). These samples were concurrently analyzed on the BioFocus 2000. Intraassay and interassay CVs for the five fractions for a normal sample were 0.17-1.44% and 0.42-9.11%, respectively, and 0.21-3.37% and 0.29-3.61%, respectively, for a sample with monoclonal gammopathy. Correlation coefficients for albumin and the albumin/globulin (A/G) ratio were 0.8891 (albumin range 17-93 g/L) and 0.8276 (A/G ratio range 0.39-7.81), respectively. The A/G ratio alone could not discriminate between the three groups. Capillary zone electrophoresis (CZE) correctly identified 33 of 36 samples in the normal group, and 22 of 24 samples in the other two groups, giving a clinical sensitivity and specificity of 91.7%. Our preliminary evaluation shows that protein separation by CZE is a simple, rapid, and automated alternative to conventional AGE.

Adult↗
PubMedDate not supplied

Polymerization shrinkage of densely-filled resin composites.

A new group of restorative materials called "packable" composites has recently been introduced. These products are essentially highly-filled or densely-filled hybrid resin composites. One of the many claims made about these materials is that they undergo less polymerization shrinkage than their conventional counterparts. This in vitro investigation compared the amount of linear shrinkage that occurs within a variety of densely filled resin composites (DFC) and conventional hybrid resin composites when cured with a visible halogen light. Six DFC resins (Alert, Ariston, P60, Prodigy, Solitaire and Surefil) and two hybrids (TPH-Spectrum, Z100) were used in this study. Dimensional change was measured in a linear direction using a calibrated light microscope. Eighty samples of resin composite were tested, resulting in eight groups of 10 samples (N=10) each. The one-way ANOVA with Student-Newman-Keuls post-hoc test was used to compare the shrinkage between groups, and Pearson's Correlation was used to test the relationship between filler characteristics and shrinkage. Alert and P-60 had significantly less shrinkage than Solitaire, Ariston, Prodigy, Z-100 and TPH-S. Thus, the shrinkage values of some DFC resins were significantly less and others were no different from conventional hybrid resins. There is a moderate association between filler volume and shrinkage. Filler size and resin chemistry are other factors that may also effect shrinkage.

Analysis of Variance↗
PubMedDate not supplied

Polymerization shrinkage of composite resins using plasma-arc photocuring.

When composite resins are photocured, they undergo a certain degree of polymerization shrinkage. A new method utilizes plasma-arc photocuring instead of conventional visible light. The purpose of this in vitro investigation was to compare the amount of linear shrinkage that occurs when a hybrid composite resin is cured using three different plasma-arc light (PAL) sources. There was no significant difference between the three groups and a halogen light control in the amount of shrinkage of the hybrid composite resin. In terms of resin shrinkage values, using a PAL is no different than using a conventional halogen curing light. However, even though the curing intensity is increased, the shorter curing times may result in a reduced depth of cure.

Analysis of Variance↗