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T Bamba

Publications and source records attributed to T Bamba.

At least 181 records · Page 10Linked to original sources
PubMed1990

[Effect of pancreatico-biliary diversion on endogenous CCK secretion, pancreatic enzyme synthesis, and amylase release].

Pancreatico-biliary diversion (PBD) by jejunal transposition in rats caused pronounced hyperplasia of pancreas. The increase of pancreatic trypsinogen contents exceeded the pancreatic growth, while pancreatic lipase and amylase contents relatively reduced. The high level of plasma CCK at the early period of PBD gradually decreased. The plasma secretin levels remained unchanged for 14 days after PBD. The plasma CCK levels were not elevated in fasting, but increased after intrajejunal infusion of 0.1 N HCl or Clinimeal. In acini prepared from PBD rats, the responsiveness to CCK8 was decreased when amylase release was expressed relative to DNA. The dose-response curve for CCK8 was shifted 3 fold toward higher concentrations of CCK8 4 days after PBD, but on 7 and 14 days after PBD returned to the same curve as the transected rats (control).

Amylases↗
PubMed1990

[Flow cytometric analysis of immunophagocytosis using sensitized fluorescent microspheres bearing C3b].

We analyzed the phagocytic activity of purified human monocytes using fluorescent latex beads sensitized with IgG or IgG.C3 by flow cytometry. To prepare IgG-sensitized latex beads (BA), BSA-coated latex beads (B) were incubated with diluted rabbit IgG anti-BSA. To bind complement components, BA were incubated with whole serum pretreated with K-76 monocarboxylic acid (K-76COOH). K-76COOH inhibits the activity of factor I and C5, resulting in deposition of C1, C4b, C2a, C3b on BA (BAC). Phagocytic activity was assessed by percent phagocytosis and phagocytic index (PI). To eliminate the effects of non-phagocytosed latex beads, subtraction of the data at 4 degrees C from 37 degrees C was performed. Percent phagocytosis for 60 min. was B 5.0%, BA 18.3%, and BAC 57.5%, and PI (ingested latex beads/100 cells) was B 7.9, BA 36.8, and BAC 152.7, respectively. In addition, K-76COOH caused dose dependent inhibition on IgG.C3 mediated phagocytosis. Comparison of inhibition pattern on BAC and BA indicated that K-76COOH directly inhibited C3.C3-receptor binding.

Complement C3b↗
PubMed1989

Effects of pectin on fatty acid and glucose absorption and on thickness of unstirred water layer in rat and human intestine.

Effects of pectin, a soluble dietary fiber, on fatty acid and glucose absorption were studied in vivo in rats and humans by perfusing the intestine with linoleic acid and glucose solutions with and without pectin. Linoleic acid and glucose absorption decreased with increasing concentrations of pectin. The reduction in linoleic acid absorption was not caused by binding of linoleic acids by pectin or impaired micelle formation due to binding of bile acid by pectin. The unstirred water layer expanded with increasing concentrations of pectin. These results suggest that enlargement of the unstirred water layer is closely associated with the reduction of absorption of fatty acid and glucose ingested with pectin.

Adult↗
PubMed1989

Effect of pectin, a soluble dietary fiber, on functional and morphological parameters of the small intestine in rats.

We investigated the effects of pectin, a soluble dietary fiber, on functional and morphological parameters of the small intestine in rats. A control group and a pectin-fed group were given a fiber-free elemental liquid diet and an elemental liquid diet containing 2.5% (w/w) pectin, respectively, for 2 weeks. The ileal mucosal specific activities of maltase, sucrase and alkaline phosphatase increased significantly in the pectin-fed group. Maltose absorption of the ileum, studied in vitro by the method of everted sacs and disaccharide-dependent potential difference, increased significantly in the pectin-fed group. The length of the small intestine as well as the villus height and crypt depth of both the jejunum and the ileum were significantly greater in the pectin-fed group. The crypt cell production rate of the jejunum and the ileum was also significantly greater in the pectin-fed group. Plasma enteroglucagon, but not gastrin, increased significantly in the pectin-fed group. These data suggest that pectin feeding results in hyperplasia of the small-intestinal mucosa and a significant increase in the enzyme activities of the brush border membrane of the ileum.

Alkaline Phosphatase↗
PubMed1989

[Study on the trophic effect of camostate mesilate on ethionine-induced pancreatic injury rat].

Pancreatic injury was induced to rats with intraperitoneal injection of ethionine 60 mg per 100 g BW twice or three times weekly for 6 weeks. These rats were given 100 mg/kg of Camostate mesilate (CM) via a gastric tube daily for 14 days. CM administration resulted in an increase of pancreatic wet weight, hypertrophy and hyperplasia of acinar cells, and an increase of exocrine pancreatic function. Acini prepared from CM and ethionine-treated rats exhibited increased response to caerulein, but decreased sensitivity to caerulein. The plasma CCK level in rats with CM administration 24 hours later was higher than that without CM administration. However, there were no significant changes in plasma CCK and secretin level thereafter. We concluded that CM had a trophic effect on the pancrease with ethionine-induced pancreatic injury, and CCK was considered playing the same role in injured pancreas as the normal rat pancreas. Studies using CCK receptor antagonist are needed for further clarification.

Administration, Oral↗
PubMed1989

[Effect of feeding a small-peptide elemental diet on amino acid absorption and its mechanism--study by measuring the transmural potential difference in guinea-pig small intestine].

The effect of feeding a small-peptide elemental diet on brush border membrane aminopeptidase activities and amino acid absorption in guinea-pigs was investigated. The latter effect and its mechanism were studied by measuring the transmural potential difference. Aminopeptidase activities on brush border membrane were significantly higher in the group fed a small-peptide elemental diet (SP group) than in the group fed an amino acids elemental diet (AA group). In the SP group, the absorption of both glycyl-L-leucine and L-leucine was greater than that in the AA group. The increase of delta PDmax of L-leucine in the SP group indicated that the acceleration of mucosal uptake of an amino acid in the SP group was due to an increased number of carriers.

Amino Acids↗
PubMed1989

[Effect on uptake of D-glucose, L-leucine and L-leucylglycine into intestinal brush border membrane vesicles isolated from rats fed either oligopeptide or amino acid elemental diet].

The uptake of D-glucose, L-leucine and L-leucylglycine into intestinal brush border membrane vesicles isolated from rats was investigated. They were fed either oligopeptide (SP group) or amino acid (AA group) elemental diets for four weeks. The results are as follows. As for L-leucine uptake, there was no significant difference in Km value between SP group and AA group. But Jmax value of SP group is significantly 1.7 times as large as that of AA group. As for L-leucylglycine uptake, there was no difference in Km value between the two groups, but Jmax value of SP group is significantly about twice as large as that of AA group. There is however, no difference in Km value and Jmax value between the both groups. These results indicate that L-leucine and L-leucylglycine transport carriers in the brush border membrane significantly increased in SP group compared with that in AA group, and that these carriers might be induced by oligopeptides as intraintestinal substrate.

Amino Acids↗
PubMed1985

Clostridium ramosum, an IgA protease-producing species and its ecology in the human intestinal tract.

A bacterial strain isolated from feces of a patient with ulcerative colitis, which had been shown to produce a novel immunoglobulin A (IgA) protease (cleaving both the human IgA1 subclass and IgA2 subclass of A2m(1) allotype) extracellularly, was identified as Clostridium ramosum. By using a selective medium (propionate-rifampicin-gentamicin-colimycin-polymyxin medium) devised for C. ramosum, analysis of the population level of this organism was performed to determine its ecology in the human intestinal tract. C. ramosum was isolated in 20 of 25 fecal samples (80%) from patients with inflammatory bowel disease (I.B.D.) and in 112 of 135 samples (83%) from patients without I.B.D. (control group). C. ramosum was also isolated from 6 of 11 biopsy samples (55%) of the inflamed rectal mucosa from patients with ulcerative colitis and from five of 15 samples (33%) from the intact mucosa of the control group. The population levels of C. ramosum in most of the biopsy samples ranged from 2.3 to 5.0 log10 per gram. The IgA protease-positive C. ramosum was found in only four of 135 fecal samples (3%) and one of 15 biopsy samples (6.7%) from the control group. These results indicate that IgA protease-positive C. ramosum is not likely to play a role in the induction of I.B.D., unless the organism is first isolated from the patient with I.B.D.

Clostridium↗
PubMed1985

A novel IgA protease from Clostridium sp. capable of cleaving IgA1 and IgA2 A2m(1) but not IgA2 A2m(2) allotype paraproteins.

Three bacterial strains of Bifidobacterium and Clostridium sp. from patients with inflammatory bowel disease (I.B.D.) and Streptococcus pneumoniae from a patient with pneumonia were identified to produce extracellular proteases cleaving IgA into Fab and Fc fragments. Although the proteases from the Bifidobacterium and the Streptococcus pneumoniae showed the characteristics of typical IgA1 proteases, cleaving the IgA of only the IgA1 subclass, the protease from Clostridium sp. revealed a dual substrate specificity, in that it cleaved both IgA1 and IgA2 of the A2m(1) allotype. The latter protease, however, did not show any activity with respect to the IgA2 of the A2m(2) allotype. Fc fragments isolated from the IgA1 and the IgA2 A2m(1) by digestion with the Clostridium sp. protease were identified to have an identical amino terminal residue of valine. The site of cleavage in both the alpha 1 and the alpha 2 of A2m(1) by the protease was assumed to be an identical peptide bond at Pro(221)-Val(222), which is a common one present just before the hinge of both the alpha 1 and the alpha 2 of the A2m(1) but not of the alpha 2 of the A2m(2). The protease was sensitive to ethylene-diamino tetraacetic acid, a chelating agent, similar to other already reported IgA1 proteases.

Bacterial Infections↗
PubMed1976

Tissue plasminogen activator in the pathogenesis of protein-losing gastroenteropathy.

Tissue fibrinolytic activity, mainly due to activator of tissue plasminogen was increased in the biopsied gastric mucosa of patients with protein-losing gastropathy. Antiplasmin therapy with trans-AMCHA (trans-4-aminomethyl cyclohexane carboxylic acid) was dramatically effective, except in the case of a patient with intestinal lymphangiectasia. These findings suggest that increased fibrinolysis in the gastrointestinal mucosa may play an important role in enhancing mucosal permeability to plasma proteins.

Adult↗
PubMed1976

Experimental protein-losing gastroenteropathy: role of tissue plasminogen activator.

The tissue fibrinolytic activity of intestinal mucosa was studied in rats with protein-losing gastroenteropathies experimentally produced by X-irradiation, 5-fluorouracil treatment, or ligation of the thoracic duct. The first two models showed an increase of fibrinolytic activity and of 131I-polyvinylpyrrolidone excretion and the third showed normal fibrinolysis. In addition, it was demonstrated that 131I-polyvinylpyrrolidone excretion in the first two groups was markedly reduced by injections of trans-4-aminomethyl cyclohexane carboxylic acid, a potent antiplasmin agent. This finding affords strong support to the authors' previous clinical observation that tissue fibrinolysis in the digestive mucosa plays an important role in the pathogenesis of protein-losing gastroenteropathy associated with diseases of the mucosa.

Animals↗