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Biomedical subjects

T Arima

Publications and source records attributed to T Arima.

At least 289 records · Page 16Linked to original sources
PubMed1985

Solid-phase radiolabeling of glycolipids with galactose oxidase and sodium boro[3H]hydride.

Neutral glycosphingolipids immobilized on plastic surfaces were specifically radiolabeled with the galactose oxidase-sodium boro[3H]hydride method. Glycolipids could be immobilized in the absence of any other lipids such as phosphatidylcholine and cholesterol. Rather, the presence of these lipid components decreased the efficiency of radiolabeling. Glycolipids having more than four carbohydrate moieties with terminal galactosyl residues were effectively radiolabeled and could be easily recovered by elution from the plastic with ethanol in one step. This method will be suitable for analysis of small samples of "upper-phase" neutral glycolipids having terminal galactose or N-acetylgalactosamine.

Animals↗
PubMed1985

Three-dimensional features of the striated organelle in the vestibular hair cell.

The striated organelle, the Friedmann's body, of the vestibular hair cells in normal guinea pigs were examined using a freeze-fracture-etch technique. The striated organelles were composed of periodic parallel loose spirals of two fine fibrils, the thick strands. This main distance was divided by thin strands, and many fine fibrils cross-linked the thick strands to the central thin strands. Stereo-micrography showed that the thin strands were positioned on a different plane from that of the thick strands and that the thin strands were cross-linked with thick strands by fine fibrils in a radiating pattern, giving them a columnar appearance.

Animals↗
PubMed1985

Human hepatocellular carcinoma is associated with quantitative and qualitative changes in glycolipids.

Changes of glycolipids in the naturally occurring human hepatocellular carcinoma tissues were studied. Neutral glycolipids (two fractions) and gangliosides from human hepatocellular carcinoma tissues and their adjacent uninvolved liver tissues from ten patients were purified and analyzed by high-performance thin-layer chromatography. Human hepatocellular carcinoma was shown to be associated with the extensive changes of the thin-layer chromatography patterns of both neutral glycolipids and gangliosides. Changes in neutral glycolipids were: (a) increased dihexaosylceramide with the concomitant decrease of trihexosylceramide and globoside, (b) increased ratio of the lower band of globoside to the upper band, and (c) increased expression of at least two "upper-phase" neutral glycolipids. Changes in gangliosides were: (a) increased expression of gangliosides migrating slower than GM3 on thin-layer chromatography with relative decrease of GM3, and (b) expression of tumor-associated new gangliosides, i.e., "neogangliosides".

Adult↗
PubMed1984

A deep-etching study of the guinea pig tracheal cilium with special reference to the ciliary transitional region.

The fine structure of the cilium was examined by freeze-fracture-etch studies. In the interior of the transitional region, three types of plate structures were clearly observed. While the terminal plate contained fine fibrillar linkers suspending the central core plates from its peripheral doublet microtubules, two other types of plates had no suspending linkers. At the upper level of transitional region, one of the central microtubules elongated deeper than the other in the space surrounded by ring structure. Axosome-like structure was not observed in our replicas. Central vesicle of the basal body was also suspended by fine fibrillar linkers from peripheral triplets. Though membrane particles of ciliary necklace were recognized on protoplasmic and external fracture faces, and the external surface, particle arrays were not observed on protoplasmic surface. Instead, Y-shaped, cross bridges, one end of which attached to the doublet microtubules, merged in the circular ridge structure at opposite ends. This circular ridge structure at the necklace region may play a role as an anchoring site of both membrane particles of the necklace and cross bridges from peripheral doublet microtubules.

Animals↗
PubMed1984

Dynamics of swallowing in tetanus.

An 82-year-old man developed an uncommon type of swallowing disturbance with tetanus. Initially, he could masticate his food but could not pass it into the esophagus. The mechanism of swallowing in this patient was studied radiologically and endoscopically. The disturbance in the second stage of deglutition was associated predominantly with his advanced age and the slow clinical progress of the tetanus. The patient was cured by treating the disease.

Aged↗
PubMed1984

Double-axis rotary replication for deep-etching.

We have developed a simple new method of rotary shadowing, double-axis rotary replication, for observing three-dimensional structures in deep-etched, rapid-frozen tissues. The technical details of this method are described and compared with the conventional fixed-angle rotary shadowing procedure.

Animals↗
PubMed1984

High-dose chemotherapy with frozen autologous marrow transplantation in patients with poor-prognosis tumors.

One hundred and ninety patients with poor-prognosis tumors, mainly advanced solid cancers were treated with a first course of high-dose chemotherapy. One hundred nine of them received frozen autologous bone marrow transplantation (FABMT) a total of 160 times. Thirty-four of the 109 patients were given a second course, and five of the 34 received a third course followed by FABMT. Eighty-four patients (77%) showed subjective and/or objective remissions; however, 59 (54%) died from the original malignant tumor, 27 (23%) from profound acquired immune deficiency, and the remaining 18 patients are now in follow-up. A comparison between the patients who received the high-dose chemotherapy followed by FABMT and those who received the high-dose chemotherapy alone because of the absence of marked myelosuppression remains as a future subject for study. We experienced some important cases, in which FABMT played a decisive role in rescuing the marrow abrasion from toxicity of the anticancer drugs. We feel certain that FABMT can accelerate hemopoietic recovery in cancer patients after high-dose chemotherapy. We also conclude that FABMT is an interesting approach to provide hope to the poor-prognosis patients who have failed or respond to conventional chemotherapy and radiotherapy, although FABMT is still undergoing clinical trial and only in specialized hospitals.

Adult↗
PubMed1983

A freeze-fracture study of perinatal changes of intramembranous particles in microvilli of absorptive cells in mouse small intestine.

Perinatal changes in the appearance of intramembranous particles (IMPs) of microvilli of enterocytes were analyzed quantitatively. In both the jejunum and the ileum, the IMP density on the P-face showed no significant changes from day 17 of gestation to day 5 of postnatal life. It increased between day 5 and day 12, reached a maximum at day 21, and thereafter decreased slightly. The IMP density on the E-face remained almost constant during the perinatal period in both intestinal parts. Measurements of particle diameters proved that neither the P-face nor the E-face membrane showed significant differences in either mean value or size distribution among different age groups. This study has revealed that the perinatal change in the IMP density on the P-face of microvilli correlates well with changes in the activity of certain enzymes found in the membranes of microvilli, e.g. disaccharidase and aminopeptidase.

Alkaline Phosphatase↗
PubMed1983

Hepatocyte plasma membrane antigens. II. Characterization of liver-specific membrane lipoprotein (LP-1) and Tamm-Horsfall glycoprotein (THGP) like antigens (hepatic THGP) on the plasma membrane of Chang liver cell.

Radioiodinated cell-surface antigens of Chang liver cells recognized with anti-liver-specific membrane lipoprotein (anti-LP-1) and anti-Tamm-Horsfall glycoprotein (anti-THGP) rabbit antibodies were analyzed by the immunoprecipitation followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The analysis revealed that the antigens precipitated with anti-LP-1 were heterogeneous in molecular species consisting of 2 major and 5 minor polypeptides with molecular weights ranging from 36 X 10(3) (36 K) to 250 K daltons. That with anti-urinary THGP was a single polypeptide with a molecular weight of 67 K daltons, which was different from urinary THGP in molecular size, and was designated hepatic THGP.

Antigens, Neoplasm↗
PubMed1983

Studies on liver specific antigens--partial purification, characterization, and immunization.

Seven liver specific antigens, liver protein-I, liver protein-II, liver specific antigen (Espinosa), F-antigen, hepato-renal antigen, liver cell membrane antigen (Ray) and Tamm-Horsfall glycoprotein were partially purified from human liver and urine. Anti-sera against these antigens were raised in rabbits. Anti-sera against liver protein-I, liver protein-II, liver specific antigen (Espinosa) and liver cell membrane antigen (Ray) were absorbed by human serum, kidney, lung and spleen while anti-sera against hepato-renal antigen and Tamm-Horsfall glycoprotein were absorbed by human serum, lung and spleen to remove antibodies against non-specific antigens. There were no cross reactivities among these antibodies.

Animals↗