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Biomedical subjects

T Arao

Publications and source records attributed to T Arao.

At least 55 records · Page 3Linked to original sources
PubMed1989

An immunohistochemical and immunoelectron microscopic study of the ontogeny of rat Langerhans cell lineage with anti-macrophage and anti-Ia monoclonal antibodies.

An immunohistochemical study with anti-macrophage and anti-Ia monoclonal antibodies was performed to clarify the relationship between Langerhans cells (LC) and indeterminate cells (IC) in rat epidermis both in adulthood and in the fetal stage. On immunoelectron microscopy, a mouse anti-rat macrophage monoclonal antibody, TRPM-1, recently produced by us, reacted with IC and some LC in adult rat skin. Ontogenic study revealed that TRPM-1-positive cells first appeared in the epidermis of fetal rat heads on Day 17 of gestation and then spread caudally along the anterior-posterior axis. On Day 20 of gestation, when the distribution of the TRPM-1-positive cells over body surface became even, Ia-positive cells appeared in the epidermis and began to increase in number. Ia-positive cells with Birbeck granules were found on Day 21 of gestation. These results indicate that. TRPM-1-positive IC matured into Ia-expressing LC after being exposed to microenvironmental change during the perinatal period. The number of Ia-positive cells exceeded that of TRPM-1-positive cells at around 5 d after birth. Afterwards, there were more dendritic Ia-positive cells found in the interfollicular areas than TRPM-1-positive ones. However, local concentrations of the TRPM-1-positive IC in the follicular infundibula were frequently found in the fetal stage and occasionally in adulthood. These TRPM-1-positive cells in the follicular infundibula were thought to be a precursor pool in the epidermis for LC.

Aging↗
PubMed1989

Proliferating giant pigmented nevus: a report of an unusual tumor occurring in association with a congenital giant pigmented nevus.

A 32-year-old Japanese woman with a giant pigmented congenital nevus of the torso presented with a massive pigmented tumor mass of the vulva which grew over an 8-year period. Histologically, the tumor was composed of benign appearing nevus-like cells with focal areas of extensive fibrous response. The tumor cells were positive for S-100 protein and with an antihuman melanoma antibody (MoAb 225, 28S) stain. Electron microscopy confirmed the nevomelanocytic nature of the tumor cells and demonstrated peculiar cytoplasmic crystalline tubular structures similar to those seen in cells infected with herpes virus type II. We propose the term "proliferating giant pigmented nevus" for this previous undescribed tumor.

Adult↗
PubMed1989

Pigmented basal cell carcinoma developing on the lower extremities--three cases masquerading as malignant melanoma.

Three cases of pigmented basal cell carcinoma were reported which needed to be differentiated from malignant melanoma based on their location and clinical features. Clinicopathologically, they were characterized by a large number of melanophages in the stroma, blockade melanocytes in the parenchyma, and phagocytosis of melanosomes in tumor cells as well as a large number of intraparenchymal melanophages. Two cases were characterized by the aggregation of melanophages in cystic spaces caused by stellate atrophy.

Aged↗
PubMed1989

[Atypical myrmecia--on the importance of the anatomical site of HPVs-infection as a morphological factor].

An 11-year-old Japanese girl developed a horny tumor branching into three parts in the fourth interdigital fold of the left hand; histologically it had numerous eosinophilic granular inclusion bodies. A histological diagnosis of myrmecia was made, which was further confirmed by the demonstration of human papillomavirus type 1 (HPV-1) by the in situ hybridization method. However, the clinical picture did not resemble the so-called myrmecia. A review of other atypical cases of myrmecia on areas other than the palms and soles may indicate the role of topical factors in determining the shape of viral warts. The concept of myrmecia should be revised in view of the additional information that there is histological heterogeneity in the cytoplasmic inclusion bodies, which are the basis of the definition of myrmecia.

Child↗
PubMed1989

[A new type of viral wart? A case of viral wart having intracytoplasmic filamentous substances].

Reported was a 16-year old Japanese girl suffering from peculiar viral warts on the soles. All lesions showed whitish punctate keratotic appearance. Three biopsy specimens taken from one patient were found to have particular intracytoplasmic basophilic eosinophilic filamentous substances in each cell. These intracytoplasmic substances were quite different from those of myrmecia, which was described as inclusion wart by Lyell and Myles. Myrmecia is caused by human papillomavirus type 1 (HPV-1) and its intracytoplasmic substances are characterized by numerous intracytoplasmic eosinophilic granules. In spite of that immunohistologically papillomavirus common antigens were detected, HPV-1,2,4 was never detected by in situ hybridization method in our case. We assume that our case is a new type of viral wart.

Adolescent↗
PubMed1988

Structure of the mouse C-reactive protein gene.

A genomic DNA clone corresponding to the mouse C-reactive protein (CRP) has been isolated and characterized. The mouse CRP gene is 1.9-kilobase pairs in length and contains a single intron of 213-base pairs which interrupts the codon for the 2nd amino acid residue of the mature CRP protein. We compared nucleotide sequences of the mouse and human CRP genes and discussed structures of possible regulatory sequences. With this characterization, the isolation and sequence analyses of a set of mouse and human pentraxin genes, i.e. CRP and serum amyloid P component genes is not complete.

Amino Acid Sequence↗
PubMed1988

Complicating systemic amyloidosis in dystrophic epidermolysis bullosa, recessive type.

An autopsy case of dystrophic epidermolysis bullosa, recessive type, complicated by systemic secondary amyloidosis is described. The patient had developed multiple bullous lesions and erosions from birth, followed by repeated infection. At autopsy, chronic persistent inflammation was observed in the skin and in various visceral organs, accompanied by systemic amyloidosis. By the peroxidase-antiperoxidase (PAP) method, amyloid deposits stained positively for anti-AA-protein antiserum. In the present case, we concluded that the systemic amyloidosis was of the AA type, and developed secondarily to the chronic persistent inflammation in the prolonged course of dystrophic epidermolysis bullosa, recessive type.

Adolescent↗
PubMed1988

[Cutaneous-internal malignancy syndrome associated with specific skin lesions].

Skin changes associated with internal malignancies can be classified into five groups: 1) cutaneous metastasis from internal malignancies, 2) non-specific reactional skin changes caused by toxic or allergic processes due to these malignancies, including pruritus cutaneous, urticaria and so on, 3) specific skin changes or diseases closely related to the malignancies, including acanthosis nigricans, dermatomyositis, Leser- Trélat syndrome, 4) the syndrome caused by simultaneous effects of oncogenesis both on the skin and on the internal organs, including multiple Bowen's disease, and 5) congenital hereditary syndrome, including Gardner syndrome, Torre syndrome. It is appropriate for the cutaneous internal malignancy syndrome to include above groups 3) 4) 5), because of the close and specific interrelation between the skin changes and internal malignancies. Two cases of glucagonoma syndrome, 2 cases of dermatomyositis with malignancy and one case of Leser- Trélat syndrome were described as typical cutaneous-internal malignancy syndrome. Characteristics findings of these skin changes suggest the presence of particular internal malignancies in cases, and the study of the etiologic process of these skin changes will be useful in the basic oncological investigation.

Adult↗
PubMed1986

Isolation and characterization of the complete complementary and genomic DNA sequences of human serum amyloid P component.

Complementary and genomic DNA clones corresponding to the human serum amyloid P component (SAP) mRNA have been isolated and analyzed. The nucleotide sequences of the cDNA and the corresponding regions of the genomic SAP DNA reported here were identical, and revealed that after coding for a signal peptide of 19 amino acids and the first two amino acids of the mature SAP protein, there is one small intron of 115-base pairs (bp), followed by a nucleotide sequence coding for the remaining 202 amino acid residues. The SAP gene has an ATATAAA sequence 29-bp upstream from the cap site, but there is no CAAT box-like sequence. A possible polyadenylation signal sequence, ATTAAA, was found to be located 28-bp upstream from the polyadenylation site. A comparison of the genomic SAP DNA sequence with that of human C-reactive protein (CRP) revealed a striking overall homology which was not uniform: several highly conserved regions were bounded by non-homologous regions. This comparison provides further support for the hypothesis that SAP and CRP are products of a gene duplication event.

Amino Acid Sequence↗
PubMed1985

Immunohistochemical analysis of antimelanoma monoclonal antibodies, with special reference to fetal tissue distribution.

An immunohistochemical study using 2 antihuman melanoma monoclonal antibodies designated as MoAb 225.28S and MoAb 653.40S was carried out on various human skin tumors, including malignant melanoma as well as on normal and fetal tissues by indirect immunofluorescence technique. Specific immunofluorescence was observed not only in malignant melanoma cells but also in cells of pigmented nevi, basal cell epithelioma, normal hair follicles, and some fetal tissues. Both monoclonal antibodies were revealed to be able to recognize the common antigenic determinant shared by several skin tumors, including malignant melanoma, and fetal tissues. Therefore, both monoclonal antibodies might recognize premature antigen of both melanocytic and keratinocytic cell lineage.

Antibodies, Monoclonal↗
PubMed1985

[Other malignant skin tumors: carcinoma of cutaneous appendages and metastatic carcinoma to the skin].

Carcinoma of cutaneous appendages, which are derived from hair follicles, sebaceous glands and sweat glands, are generally rare. Although no papers on malignant tumors derived from hair follicles have been reported either at home or abroad, several cases of distinct hair follicle carcinoma have recently been reported in this country. In this paper, malignant change of epidermoid cyst (follicular), malignant proliferating trichilemmal tumor, malignant change of trichilemmoma, and BCC-like tumor associated with immature hair follicle formation, were discussed as several types of malignant changes occurring in the hair follicle. Both sebaceous and sweat gland carcinomas, frequently show a relatively poor prognosis due to a marked tendency for widespread metastasis. In apocrine gland carcinoma appearing in axillar and chest areas, there is a difficulty in making differential diagnosis from carcinoma of latent accessory mammary gland. An outline of metastatic carcinoma to the skin was given.

Aged↗