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Biomedical subjects

T Aoki

Publications and source records attributed to T Aoki.

At least 1,225 records · Page 68Linked to original sources
PubMed1974

Surface antigens of mammalian sarcoma virus-transformed nonproducer cells.

In vitro, clonal BALB/3T3 mouse cells were transformed into nonproductive sarcoma cells by Kirsten murine sarcoma virus. The resulting cells are called K-BALB. Similarly, the Kirsten sarcoma virus and woolly monkey sarcoma virus transformed normal rat embryonic kidney (NRK) cells to nonproductive sarcoma cells K-NRK and W-NRK, respectively. The presence of sarcoma cell surface antigen (SCSA) on these three different cell lines has been examined by immunoelectron microscopy using the rabbit anti-K-BALB, anti-K-NRK, and anti-W-NRK sera preabsorbed with normal embryonic rat cells, NRK cells, and BALB/3T3 cells. Four different specific sarcoma cell surface antigens have been demonstrated: SCSAa, an antigen specific to the surface of W-NRK cells; SCSAb, a surface antigen common to K-NRK and W-NRK cells; SCSAc, a surface antigen common to K-BALB and W-NRK cells; and SCSAd, a surface antigen broadly reactive with the rabbit antisera to W-NRK, K-NRK, and K-BALB.

Animals↗
PubMed1974

Detection of R factors in naturally occurring Vibrio anguillarum strains.

R factors were detected in Vibrio anguillarum strains from vibrio-diseased freshwater fish, ayu (Plecoglossus altivelis), in Japan. It was found that 65 out of the 68 random isolates from epidemics of vibrio disease in 1973 carried transferable drug resistance factors. The most common type determined resistance to sulfonamides, streptomycin, chloramphenicol, and tetracycline and belong to the fi(-) type. The high incidence of R factors in Vibrio anguillarum from cultured fish is assumed to be due to selective pressure exerted by chemotherapeutic agents used in fish culturing.

Drug Resistance, Bacterial↗
PubMed1974

Detection of naturally occurring antibodies to RNA-dependent DNA polymerase of murine leukemia virus in kidney eluates of AKR mice.

Specific antibodies to the RNA-dependent DNA polymerase (reverse transcriptase) of murine type C viruses have been isolated from the renal glomeruli of both leukemic and nonleukemic AKR mice where they presumably had been deposited as immune complexes. The antibodies were shown to have sedimentation coefficients of 26S to 28S and 5S to 7S on sucrose rate zonal centrifugation. Inactivation with monospecific antisera to various mouse immunoglobulins identified antibodies as being in both immunoglobulin (IGM) and IgG classes. In addition, these antibodies only reacted with the reverse transcriptase from murine and feline type C viruses, but not the polymerase from avian myeloblastosis virus (AMV). Our results provide additional evidence for the lack of immunological tolerance and demonstrate the presence of another immune complex system in AKR kidneys.

Animals↗