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Biomedical subjects

T Amano

Publications and source records attributed to T Amano.

At least 397 records · Page 22Linked to original sources
PubMed1978

Establishment of mouse neuroblastoma clone N18, rat glioma clone C6, and rat striated muscle clone L6 in serum-free, chemically defined medium.

Mouse neuroblastoma clone N18, rat glioma clone C6, and rat striated muscle clone L6 were grown in a serum-free Dulbecco's modified Eagle's medium. Their doubling times were 48 hours, 40 hours, and 4 days, respectively. Morphologic features were similar to the original parent cell lines. Membrane components of N18 and C6 grown in serum-free medium were compared with the original parent cell lines. Defects of several membrane proteins were found with sodium dodecyl sulfate--polyacrylamide gel electrophoresis.

Animals↗
PubMed1977

Acetylcholine receptor of a myogenic cell line, L6.

The biochemical characters of acetylcholine receptor (AChR) in a myogenic cell line, L6, are as follows. 1. AChR extracted with Triton X-100 has a molecular weight of 5.5 X 10(5) determined by gel-chromatography, an apparent S value of 9.7 determined by sucrose density gradient centrifugation, and an isoelectric point of 5.3. AChR is shown to interact with concanavalin A. 2. The association rate constant of the binding reaction between AChR and alpha-bungarotoxin (alpha-BGT) labelled with 125I is 1 X 10(6) M-1 with S-1 at 35 degrees C. Preincubation with d-tubocurarine blocks the binding. The half-life of the AChR-toxin complex exceeds 180 h.

Acetylcholine↗
PubMed1976

Electrical response of glioma cells to acetylcholine.

A bromodeoxyuridine resistant mutant of rat gliom line C6 grown in the presence of N6,02'-dibutyryl adenosine-3':5'-cyclic monophosphate, although inexcitable by electrical current, responds to iontophoretic application of acetylcholine by slow hyperpolarization. The sensitivity to acetylcholine is strongly reduced by excessive amounts of acetylcholine. The hyperpolarization response is inhibited by atropine or alpha-bungarotoxin, but not by D-tubocurarine. In the absence of acetylcholine some cells display spontaneous hyperpolarizations at regular intervals. The membrane resting potential is decreased if the concentration of external K+ is raised.

Action Potentials↗
PubMed1976

Evidence for a one-hit theory in the immune bactericidal reaction and demonstration of a multi-hit response for hemolysis by streptolysin O and Clostridium perfringens theta-toxin.

An analytical method was developed for estimating the number of hits necessary to lyse or kill cells in which various concentrations of the cells are treated with a constant amount of the lytic or killing agent in a constant reaction volume. The reaction may be due to a single-component agent or occur by a sequential chain of reactions due to a multi-component agent, even including side, abortive, or counter-reactions. It was clearly shown by this method that immune bactericidal reactions followed a one-hit theory. It was shown by this method that streptolysin O required four or five hits for hemolysis and Clostridium perfringens theta-toxin required two hits. These results were confirmed by both logarithmic dose-response and survival analyses. It was also shown that streptolysin O and theta-toxin can act complementarily on accumulation of the hits for hemolysis.

Animals↗