Biomedical subjects
Publications and source records attributed to T Akaike.
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In the lateral vestibular nucleus, vestibulospinal tract (VST) neurons were surveyed with microelectrodes in cats anesthetized with sodium pentobarbital. The VST neurons (n = 450) were classified by their properties; axonal courses (LVST and MVST). spinal segmental levels of their axonal termination (C1-3, C4-8, T1-13, L1-4, and L5-neurons), their orthodromic activation by the primary vestibular nerve (second-order and non-second-order vestibular neurons), and their location in the LVN. Inhibitory and excitatory effects of cerebellar stimulation on these classified VST neurons were investigated. 84% (259/308) neurons were observed to receive cerebellar corticovestibular inhibition. The rate was high, and almost the same among classified neurons; C1-3 to L5-neurons, and second-order and non-second-order neurons. However, the rate with MVST neurons (69%) was significantly lower than with LVST cells (87%). These neurons which received cerebellar inhibition were distributed in all areas even deep in the rostroventral region of the LVN, while neurons which did not receive were distributed in the ventral region of the LVN. Electrical stimulation of ipsi- and contralateral fastigial nuclei evoked monosynaptic excitation of the classified VST neurons. Rate of occurrence of crossed fastigiovestibular excitation was higher with cervical neurons (86%) than with lumbar neurons (43%), and higher with second-order neurons (78%) than with non-second-order neurons (41%). Neurons which received monosynaptic excitation from crossed fastigiovestibular fibers were distributed in the ventral region of the LVN. In total, 73% of the neurons were identified to receive either ipsi- or contralateral fastigiovestibular excitation. The results indicated that there was relative scarcity of fastigiovestibular projections in the dorsal region of the LVN. Spinovestibular and other afferents to the LVN were also investigated.
In the lateral and descending vestibular nucleus, vestibulospinal neurons were surveyed extra- and intracellularly in cats anesthetized with sodium pentobarbital. The neurons were investigated both by their antidromic activation from the spinal cord (C1, C4, T1, L1 and L5 spinal levels) and from the oculomotor nucleus region, and by orthodromic activation from the vestibular nerve. Axonal courses of vestibulospinal neurons were determined electrophysiologically at C1 level, as medial (MVST) or lateral (LVST). By single and the same electrodes a number of neurons were recorded in wide regions of the lateral and descending vestibular nucleus from single cats. Thus, it became possible to investigate somatotopical localization systematically for the first time with microelectrode techniques. Neurons of origin of the LVST were localized in the lateral vestibular nucleus. Second-order vestibular neurons were localized in the ventral region of the lateral vestibular nucleus, and in the rostral region of the descending vestibular nucleus. Many second-order, double discharge MVST neurons were identified in the descending and lateral vestibular nucleus. Somatotopical localization were recognized, but non-second-order cervical neurons were identified in the dorsal region, although second-order lumbar neurons were identified in the ventral region of the lateral vestibular nucleus. Specific modes of vestibular activation of these vestibulospinal neurons were discussed, and the vestibulospinal systems in the cat and rabbit were discussed.
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Unit activity of locus coeruleus (LC) neurons in rats was investigated. After the animal recovered from anesthesia, the spontaneous activity exhibited periodic bursting discharges at about 15-30 s intervals. The oscillation was observed to last for a long time (1-3 h). It is suggested that many LC neurons exhibited the oscillation synchronously during stress in the awake animal.
The bridging of IgG antibody molecules by either multivalent antigen or protein A adsorbed to a polymer surface triggers superoxide anion (O-2) generation from mouse peritoneal macrophages. Macrophages, upon stimulation with IgG adsorbed to a hydrophobic polymer, polystyrene, also significantly generated O-2. In contrast, soluble IgG did not stimulate macrophages. From these results we speculated that IgG adsorbed to polystyrene produces the same kind of a conformational change in its Fc region as seen in immune complexes, and leads to enhancement of the ability of the Fc region to bind macrophage Fc receptors, acting as a multivalent cross-linking agent to aggregate the Fc receptors. The surface charge density of the immunosorbent did not affect the antigen recognition function of antibody, but markedly affected its effector function. These findings suggest that activation of the Fc receptor-mediated function of macrophages requires, on the one hand, optimal conformational change and orientation of IgG and immune complexes adsorbed to the polymer surface and, on the other hand, the bridging of IgG molecules by multivalent agents.
ABA-type block copolymers composed of 2-hydroxyethyl methacrylate (HEMA), a hydrophilic monomer, and styrene (St), a hydrophobic monomer, were synthesized by the coupling reaction of telechelic oligomers used as prepolymers. These block copolymers may be represented as microphase-separated structures. It is therefore possible to change the balance between hydrophilicity and hydrophobicity in the level of an assembled order of macromolecules. In response to the relative composition of the copolymers, three typical morphological patterns were observed in electron microscopic photographs: dispersed domains of continuous St chains in the region of HEMA chains, alternate HEMA and St lamellae and finally, dispersed phases of continuous HEMA chains in the region of St chains. The effect of the hydrophilic and hydrophobic microdomains of the copolymers on the mode of interaction between polymers and platelets was studied by the microsphere column method. In the case of homopolymers and random copolymers, a significant degree of platelet adhesion and aggregation was observed. However, the degree of platelet adhesion and deformation was suppressed on the surfaces of the block copolymers containing 0.608 and 0.347 mole fractions of HEMA whose microdomains were hydrophilic-hydrophobic lamellae and isolated hydrophilic islands in hydrophobic areas, respectively. These results show that the microphase-separated structures were antithrombogenic and prevented platelet adhesion and deformation. On the basis of the results obtained, the interaction between platelets and polymer surfaces was described in terms of the effect of hydrophilic and hydrophobic microdomains.
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Neurons in the cerebropleural ganglia (CPG), photoreceptors in the eye, optic ganglion cells, and statocyst hair cells of the nudibranch mollusk Hermissenda crassicornis responded in specific ways, as recorded intracellularly, to stimulation of the chemosensory pathway originating at the tentacular chemoreceptors as well as to stimulation of the visual pathway originating at the photoreceptors. Synaptic inhibition of photoreceptors occurs via the chemosensory pathway. The possible significance of such intersensory interaction is discussed with reference to preliminary investigation of the animal's gustatory behavior and possible neural mechanisms of behavioral choice.
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