Bead-based cellular analysis, sorting and multiplexing.
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Animals↗
Biomedical subjects
Stifun Mittoo
Publications and source records attributed to Stifun Mittoo.
The impact of combinatorial methodologies on medicinal chemistry.
Combinatorial chemistry has had a major impact on the discovery and optimisation of potential lead compounds. This review details some of the fundamental principles behind combinatorial chemistry and describes a variety of methods employed in the search for new therapeutically interesting compounds including the concept of dynamic combinatorial chemistry as a method of selecting active compounds from a mixture. It also outlines methods used to analyse resin bound products and describes how solution phase library generation may be aided by the use of resin bound reagents and scavengers.
Combinatorial Chemistry Techniques↗
Cell penetrable peptoid carrier vehicles: synthesis and evaluation.
Using a highly efficient solid-phase route a series of fluorescein conjugated peptoid oligomers were synthesised and observed to display remarkable cell penetrating properties, offering the possibility of highly efficient cellular targeting.
Animals↗
Synthesis and evaluation of fluorescent probes for the detection of calpain activity.
Two new probes for the detection of calpain I activity based on fluorescence resonance energy transfer technology have been synthesized and evaluated. The probes incorporated the cleavage site present in alpha-spectrin, a naturally occurring substrate of calpain I. The design of the internally quenched substrates is such that the calpain-sensitive bond of the peptides (between the Tyr-Gly residues) is located centrally between the donor and the quencher chromophores. The calpain assay protocol is capable of detecting enzymatic activity in the nanomolar region.
Amino Acid Sequence↗