Biomedical subjects

Sofia Grammenoudi

Publications and source records attributed to Sofia Grammenoudi.

3 recordsLinked to original sources

PubMed2006

Cell type-specific processing of human Tau proteins in Drosophila.

Accumulation of hyperphosphorylated Tau is associated with a number of neurodegenerative diseases collectively known as tauopathies. Differences in clinical and cognitive profiles among them suggest differential sensitivity of neuronal populations to Tau levels, phosphorylation and mutations. We used tissue specific expression of wild type and mutant human tau transgenes to demonstrate differential phosphorylation and stability in a cell type-specific manner, which includes different neuronal types and does not correlate with the level of accumulated protein. Rather, they likely reflect the spatial distribution or regulation of Tau-targeting kinases and phosphatases.

Animals↗

PubMed2003

Evidence for alternative splicing and developmental regulation of the Drosophila melanogaster Mgat2 (N-acetylglucosaminyltransferase II) gene.

We present a molecular study of the Drosophila melanogaster Mgat2 gene that codes for the N-acetylglucosaminyltransferase II. Isolation and analysis of two cDNA clones indicated that the gene is alternatively spliced, generating two transcripts of 3.3 and 2.7kb. Developmental specificity was observed between the two alternative transcripts during the major developmental stages of D. melanogaster. The deduced amino acid sequences of the two proteins show significant homology to the equivalent mammalian proteins, especially in the carboxyterminal region. In situ hybridizations in embryos and embryonic imaginal discs showed that the gene is expressed mainly but not exclusively in the brain.

Amino Acid Sequence↗

PubMed2003

Engineering evolution to study speciation in yeasts.

The Saccharomyces 'sensu stricto' yeasts are a group of species that will mate with one another, but interspecific pairings produce sterile hybrids. A retrospective analysis of their genomes revealed that translocations between the chromosomes of these species do not correlate with the group's sequence-based phylogeny (that is, translocations do not drive the process of speciation). However, that analysis was unable to infer what contribution such rearrangements make to reproductive isolation between these organisms. Here, we report experiments that take an interventionist, rather than a retrospective approach to studying speciation, by reconfiguring the Saccharomyces cerevisiae genome so that it is collinear with that of Saccharomyces mikatae. We demonstrate that this imposed genomic collinearity allows the generation of interspecific hybrids that produce a large proportion of spores that are viable, but extensively aneuploid. We obtained similar results in crosses between wild-type S. cerevisiae and the naturally collinear species Saccharomyces paradoxus, but not with non-collinear crosses. This controlled comparison of the effect of chromosomal translocation on species barriers suggests a mechanism for the generation of redundancy in the S. cerevisiae genome.

Aneuploidy↗