Testing for Bacteria and Fungi in Cells and Ancillary Reagents-Probe-Based Quantitative PCR Assays.
This document specifies the technical elements for bacteria and fungi detection by PCR assay which enables rapid, broad-spectrum detection of bacteria and fungi with high sensitivity. Qualitative judgement is based on LOD. Key detection points include efficient nucleic acid extraction, broad-spectrum primer-probe design, high amplification efficiency and minimised background interference.
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