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Biomedical subjects

Shuai Huang

Publications and source records attributed to Shuai Huang.

3 recordsLinked to original sources

Catalytic Enantioselective Addition of Aryl, Heteroaryl, and Alkenyl Halides to Aliphatic Aldehydes.

Cobalt complexes of chiral Josiphos catalyzed enantioselective addition of aryl, heteroaryl, and alkenyl halides to a wide range of linear and branched aliphatic aldehydes, without using directing groups. The new method was easily scalable with low loadings of cobalt catalysts, and its synthetic utility was demonstrated in concise synthesis of chiral carbinols that are key motifs of drugs or synthetic precursors. The cobalt catalyst also provided high diastereoselectivity in the addition of chiral amino aldehydes. Mechanistic studies suggest cationic complexes of aryl-cobalt(II) act as active species for asymmetric insertion. Lewis acidic titanium alkoxides assist ionization of aryl-cobalt halides to form cationic complexes. The lowest-energy transition states for insertion are stabilized by π-π stacking between the migrating aryl ring and P-phenyl ring of Josiphos.

aldehydes

A spatially resolved genomic-molecular atlas of human white-matter microstructure.

Human white matter has been linked to inherited variation, circulating molecular state and brain disease, but these layers have rarely been mapped onto the same tract anatomy. Here we measured genetic effects along 6,090 atlas-aligned fiber pathways sampled at 609,000 locations in 72,185 UK Biobank participants, and integrated proteomic and metabolomic profiles within the same anatomical frame. Genetic effects were not whole-tract properties: each locus formed a spatial footprint along fiber trajectories, ranging from single locations to broad multi-tract patterns and reflecting regional polygenicity rather than tract heritability. This map identified 258, 186 and 298 previously unreported loci for fractional anisotropy, mean diffusivity and axial diffusivity; spatial patterns replicated in adults and 157 of 315 FA loci replicated in adolescence in ABCD. Mendelian randomization linked localized genetic effects to neurodegenerative and psychiatric traits, with Alzheimer's disease showing directional effects across 12 of 17 tracts. Multi-omic analyses identified 97 proteomic and 161 metabolomic associations, with the broadest signals from lipid metabolites including linoleic acid and phosphatidylcholines. The strongest lipid-metabolite and genetic signals converged in the corpus callosum, placing inherited variation, disease risk and systemic lipid metabolism on the same localized tract segments.

Journal Article

Comparative analysis of antibiotic resistance genes between fresh pig manure and composted pig manure in winter, China.

Antibiotic resistance is a critical global public health issue. The gut microbiome acts as a reservoir for numerous antibiotic resistance genes (ARGs), which influence both existing and future microbial populations within a community or ecosystem. However, the differences in ARG expression between fresh and composted feces remain poorly understood. In this study, we collected eight samples from a farm in Kaifeng City, China, comprising both fresh and composted pig manure. Using a high-throughput quantitative PCR array, we analyzed differences in ARG expression between these two types of manure. Our findings revealed significant differences in ARG profiles, as demonstrated by principal coordinate analysis (PCoA). Further analysis identified 39 ARGs (log2FC > 1, p < 0.05) in composted pig manure, with 25 genes downregulated and 14 upregulated. Notably, tetB-01, blaOCH, and blaOXY were the most abundant in composted pig manure compared to fresh manure. Additionally, 16S rRNA species profiling revealed that the composting process significantly altered the microbial community structure, with an increased abundance of Firmicutes and a decreased abundance of Bacteroidetes in composted pig manure. In summary, composting substantially transforms both the microbial community structure and the ARG profile in pig manure, underscoring its potential role in modulating the dynamics of ARGs in agricultural environments.

Animals