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Biomedical subjects

Shriniwas

Publications and source records attributed to Shriniwas.

At least 55 records · Page 3Linked to original sources
PubMed1982

Pseudomonas aeruginosa: isolation from ocular tissues. Aeruginocin typing and its relationship to corneal pathogenicity in rabbits.

Fifty strains of Pseudomonas aeruginosa isolated from human eyes were aeruginocin typed by the method of Shriniwas (1974) using ten indicator strains. These belonged to types A1 (3), B (2), F1 (1) and 14 (1). Fifteen (30%) were nontypable and 28 (56.0%) were unclassifiable presenting twenty inhibition patterns. The strains showed a wide range of antibiotic sensitivities. The highest number of insensitive strains were those obtained from corneal ulcers. Experimental keratitis produced by strains of pseudomonas was either mild, moderate or severe and was unrelated to their source. Mild keratitis was produced by types UC 7-, UC 79- and 14 isolated from pre-operative, corneal ulcer and post-operative patients. Lesions of moderate severity were produced by types 189+ & A1 whereas the severest pathology was produced by UC 23478-, B and UC 810- types. The fact that strain 23478- always produced severest pathology irrespective of whether originated from group I, II, or IV strongly suggests relationship of pathogenicity with aeruginocin type.

Animals↗
PubMed1982

An enzyme immunoassay for yeast and mycelial phase-specific antibodies in histoplasmosis.

The enzyme-linked immunosorbent assay (ELISA) has been adapted to detect class-specific antibodies to yeast and mycelial phase (MP) antigens of Histoplasma capsulatum. Experimental histoplasmosis was produced in rhesus monkeys and humoral immune responses were measured by ELISA and complement-fixation (CF) tests. Antibodies to the yeast phase (YP) reached a peak after 7 weeks and gradually declined thereafter, while MP antibodies continued to increase even after 4 months. Comparison of the 2 tests showed a significant degree of correlation but ELISA was 10-20 times more sensitive than CF. The ELISA method permitted the analysis of low levels of antibodies at early stages of infection. Applications of the ELISA technique to the serodiagnosis of histoplasmosis employing the 2 phase-specific antigens are discussed.

Animals↗
PubMed1980

A study of malabsorption in intestinal tuberculosis: stagnant loop syndrome.

A study was carried out in the patients with intestinal tuberculosis and obstruction requiring surgery to determine the pathogenesis of malabsorption in this condition. Fifteen of the 20 patients studied had malabsorption, nine of 17 (53%) had intestinal bacterial overgrowth and 10 of 16 (62.6%) had free bile acids in their jejunal aspirates. In a comparable group of nontuberculus intestinal obstruction requiring surgery, six of seven (85.7%) had malabsorption, and four of five (80%) had both the bacterial overgrowth as well as bile salt deconjugation. Among a group of 10 patients with intestinal tuberculosis without significant obstruction, four were found to have malabsorption but only one had evidence of bacterial overgrowth and bile salt deconjugation. In contrast, only one of the 10 patients with extraintestinal tuberculosis and none of the 12 healthy, normal subjects had malabsorption. None had bacterial overgrowth or bile salt deconjugation in either group. Resection of the obstructing lesion corrected the malabsorption as well as the bacterial overgrowth and the bile salt deconjugation in all four patients tested with intestinal tuberculosis. Malabsorption in intestinal tuberculosis thus appears to be associated with obstruction rather than with the tuberculous process. Demonstration of bacterial overgrowth and bile salt deconjugation in the upper small intestine of patients with intestinal tuberculosis with obstruction and malabsorption indicate the presence of a stagnant loop syndrome.

Adolescent↗