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Shigeaki Hinohara

Publications and source records attributed to Shigeaki Hinohara.

9 recordsLinked to original sources

Genotyping of triallelic SNPs using TaqMan PCR.

Methods for analysis of single nucleotide polymorphisms (SNP) are well developed. However, most ready-made SNP genotyping kits (e.g., those that use TaqMan PCR) are based on the assumption that the SNP is biallelic. Thus, most kits are unsuitable for triallelic SNPs. We have experienced difficulty genotyping an SNP using TaqMan PCR. In the present study, we developed a method of genotyping a triallelic SNP (rs3091244: alleles C, A and T) using TaqMan PCR. We used 2 different genotyping kits: one for C/A allele genotyping, and one for C/T allele genotyping. The results of these 2 kits were combined to complete the genotyping. The subjects were 320 essentially healthy elderly Japanese. The frequencies of the C, A and T alleles were 0.78, 0.155 and 0.065, respectively. This double-tube method using TaqMan SNP Genotyping Assays was very accurate and convenient, and it should be useful for genotyping in case-control association studies or linkage studies.

Aged↗

[Good Death].

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Death↗

Polymorphism of the C-reactive protein (CRP) gene is related to serum CRP Level and arterial pulse wave velocity in healthy elderly Japanese.

The objective of this study was to clarify relationships between the C-reactive protein (CRP) gene and both the serum level of CRP and arterial pulse wave velocity (PWV), using haplotype analysis of healthy elderly Japanese. Five single-nucleotide polymorphisms (SNPs) of the human CRP gene (rs1341665, rs3091244, rs1800947, rs1130864 and rs1205) were used to genotype 315 healthy elderly Japanese subjects (mean age, 77.9 +/- 4.1 years; male/female ratio, 0.96). Linkage disequilibrium was analyzed for the five SNPs. The frequency of each haplotype and diplotype was estimated using the expectation/maximization (EM) algorithm. There were statistically significant associations between the CRP level and two CRP genotypes; the p value for the T allele of rs3091244 (CT + AT + TT vs. CC + CA + AA) was 0.002 (95% confidential interval [CI], 2.1-24), and the p value for the T allele of rs1130864 (TT + TC vs. CC) was 0.002 (95% CI, 2.1-24). The only genotype that was significantly associated with arterial PWV was the C allele of rs1800947, with a p value of 0.039. The haplotype was constructed using rs1341665, rs3091244 and rs1800947, in that order. There was a significant association between the CRP level and the T-T-G haplotype, with a p value of 0.002 (95% CI, 2.1-24). There was a significant association between arterial PWV and the C-C-C haplotype, with a p value of 0.039. We concluded that rs3091244, rs1130864 and the T-T-G haplotype are genetic markers for elevated basal CRP levels. rs1800947 and the C-C-C haplotype appear to be susceptibility markers for atherosclerosis, but this requires confirmation.

Age Factors↗

Influences of age and gender on results of noninvasive brachial-ankle pulse wave velocity measurement--a survey of 12517 subjects.

The present study was conducted to evaluate the influences of age and gender on the results of noninvasive brachial-ankle pulse wave velocity (baPWV). In 12517 subjects who had no medication and no history of cardiovascular diseases, multiple regression analysis demonstrated that age, blood pressure, body mass index, triglycerides, blood glucose, and uric acid were significant variables for baPWV in both genders. From this population, we extracted 7881 "healthy subjects" (4488 males and 3393 females, 25-87 years) without any of the atherogenic risk factors, and the results of baPWV were analyzed chronologically in 5-year age intervals. baPWV was lower in females than in males until age 60, and became similar in both genders over age 60. Multiple regression analysis demonstrated that not only the value of R(2) but also the coefficient of the effect of age on baPWV are larger in females than in males. In the estimation of the regression curve, the relationship between age and baPWV demonstrated a quadratic curve in both genders. Thus, aging influences baPWV, and its effect is more prominent in female. Menopause seems to be the crucial phenomenon to explain the augmented increase in arterial stiffness with aging in females.

Adult↗

The past, present and future of International Health Evaluation Association (IHEA).

In this paper I mention the 19-year history of International Health Evaluation Association (IHEA) which was started in Washington D.C. by the great effort of Dr. G. Gilbert in Hawaii. In 1973 three regions were organized in this Association: 1) U.S.A., 2) Europe and 3) Pan-Pacific including Asia. I also mention the history of periodical health check-ups in U.K., U.S.A. and Japan. In Japan it started in 1954, however, after adapting the system of Automated Multiphase Health Testing designed by the Kaiser Foundation of Auckland, Calif., in 1973, Japanese people paid much attention to this health screening system and the number of hospitals and clinics for health screening has increased tremendously and the number of examinees amounted to 2,875,449 in 2001. Finally, I conclude that IHEA should create a multidisciplinary system to sustain a lifestyle with a high level of Quality of Life (QOL) for the people who really want to live fruitful lives by the successive health education.

Congresses as Topic↗

[Aging and QOL].

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Activities of Daily Living↗

[On the lifestyle of the aged from the view point of holistic medicine].

The population of the elderly in Japan has rapidly increasing. I want to introduce the new movement of "the new elder citizens of over the age of 75 years old". The average life expectancy of Japanese newborn is 77.10 years for males, and 84.62 for females. Yet the birth rate in Japan is low (1.22). Therefore this will further reduce the number of the productive and young age group would support the seniors. This is why the elderly support ration will come down by more than half eventually to end with 50%. In preparation for such changes to occur ageism, which is an attitude of discrimination according to age, must be vanished. Aged people should be evaluated by individual physical and mental strength, rather than by mere chronological age. Forty years ago, definition of seniors in Japan was "age 65 and over". At that time in the 1960's the average life span of the Japanese was 68 years. However, in the subsequent 40 years, the average life expectancy is in around to 81 years. So, I believe that seniors should not take care of by those from age 20 to 64; rather, they should be self-reliant and engaged in social activities. In view of this, in September 2000, I proposed a change in defining seniors. In stead of "age 65 and over", seniors should be "age 75 and over". As the result, the association for the New Elder Citizens has been organized. Those of us who turn 75 in the year 2000 were 20 years when World War II ended in 1945. I believe those seniors over 75 have a mission to tell the next generation the many lessons we have learned during wartime experiences. Besides it is recommended that those new elder citizens are encouraged to live their further life under the following slogans: 1. to love 2. to begin and create 3. to preserve (with hardships, so as to learn sympathize with others who are under strain). We already started the cohort study of coming 10 years of those new elder citizens by investigating annual physical check up and their behavior and environmental elements as well as genetic study of aging process including senile dementia. Many of these Japanese elderly people have selectively good health physically, mentally and spiritually. Japanese word "Kenko" (health) consists of two Chinese characters. The first means a state of great strength, fullness and self-support. The second character of Ken-ko means "peacefulness", or serenity of mind without stress. It means holistically healthy entity. The root of the English term "Health" goes back to the 16th century Anglo-Saxon word, "Hāl". It embodies such concepts as "whole" or "heal" or "holy". It means that a healthy state prevails in the whole human being even if individual parts of body may be afflicted in some ways. As time passed, the human body necessarily declines in its functional capacities. However the elderly persons do have high spirituality. However he or she may continue to feel a sense of fulfillment and serenity. Those are hope that even if we have an illness or a handicap, we can still be reborn as healthy individual. Being holistically healthy means that these aspects of wellness "body", "mind" and "spirit" are integrated into the well being of a whole person. The New Elder Citizens are invited to participate the cohort study for 10 years. The results would suggest that their way of living and environmental factors would reveal most important as compared with the hereditary gene factors.

Aged↗