[Progress in the olfactory system].
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Biomedical subjects
Shao-guang Fan
Publications and source records attributed to Shao-guang Fan.
[Organotypic cerebral slice culture and the course of neuronal development in it].
OBJECTIVE: To establish a method of organotypic cerebral culture. So as to pave the way for building some neurodegenerative disease models. METHODS: Organotypic cerebral cultures were prepared from prefrontal brain of neonatal SD rats. After culturing 7 to 14 days, 3 weeks, 4 weeks and 8 weeks, respectively, cerebral slices were fixed, dehydrated and sectioned in cryostat. The sections proceeded with Nissl staining and neurofilament high molecular weight (NFH) immunohistochemical staining. The difference was observed between controls and cultured slices using normal rats as controls. RESULTS: Nissl staining showed that pyramidal neurons in cultured slices were increased in volume and lightened in staining. The delaminating construction was clear from 1 to 4 weeks after culturing. In cultured slices, immunohistochemical staining showed that NFH positive pyramidal cells appeared on layer V on the tenth day and on both layers V and III after culturing 12 days. In the control group, NFH positive pyramidal cells appeared on layer V in 5-day-old rats, and appeared on both layers V and III in over 3-week-old rats. In cultured cerebral slices, the number of pyramidal neurons on layer V in M1 area was invariable from 12 days to 2 months. CONCLUSION: Orgaotypic cerebral culture can be used to study postnatal development for neocortex and build some in vitro models for neurodegenerative diseases.
Animals↗
[Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord].
OBJECTIVE: To establish an in vitro model of amyotrophic lateral sclerosis (ALS) from the organotypic culture of SD rats' lumber spinal cord induced by the mitochondrial inhibitor,malonate sodium. METHOD: The lumber spinal cord prepared from the 6-day-old SD rats was cut into 350 microm coronarily, cultured on the Millicell-CM inserts which make the spinal cord culturing on the interface between air and fluid. First, the optimum malonate sodium dose was determined by adding different doses into the medium and counting the living motor neuron numbers by using immuno-histochemistry staining. Second, the ALS model was established as following: the cultures were divided into the malonate groups and the control groups, adding 2 mmol/L sodium malonate into the medium of the malonate groups an the 3rd day, continue culture to 12 days with this concentration; the control groups culturing without malonate. RESULTS: The organotypic characteristics are still kept till the end of the curlturing. After adding the sodium malonate, counting the number of motor neurons and interneurons on the different spinal slices in the different groups, the number of motor neuron in the cultured spinal cord was less than control (11.00+/-2.45 vs 15.29+/-1.70 per semislice at the end of the culturing, P<0.01), but the difference of the interneuron was not significant. CONCLUSION: The amyotrophic lateral sclerosis model is successful with selective injury of motor neuron, and this model can be used for the exploring of the theraptic method and its pathogenesis.
Amyotrophic Lateral Sclerosis↗
[Integer multiple rhythm in the spontaneous beating rhythm of cardiac myocytes and its possible mechanism].
OBJECTIVE: To introduce the characteristics of the integer multiple rhythm of cultured cardiac myocytes and to explore the cause of its generation. METHOD: Spontaneous beating rhythms of cultured cardiac myocytes were observed with photometry system and stochastic Chay model was used to simulate the experimental results. RESULT: Integer multiple rhythm was observed in the experiment. This kind of rhythm is similar to phenomena of sinus arrest. The integer multiple rhythm similar to that of the experiments was simulated in stochastic Chay model, and was demonstrated to be induced by the mechanism of autonomous stochastic resonance. CONCLUSION: The integer multiple rhythm observed in the experiment might be generated via the effect of autonomous stochastic resonance.
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