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Biomedical subjects

S Zhou

Publications and source records attributed to S Zhou.

At least 469 records · Page 26Linked to original sources
PubMed1992

Hemophilic blood cyst--a case report.

We report a case of hemophilic blood cyst in a patient with human immunodeficiency virus (HIV) infection. The pathogenesis, diagnosis and treatment are discussed, and neither inflammatory changes nor pathological cells were seen in the numerous histological sections obtained surgically.

Bone Cysts↗
PubMed1992

Cloning and partial sequencing of the porcine growth hormone (pGH) gene from pituitary gland.

High molecular weight DNA was isolated from porcine pituitary gland, and a genomic library was constructed using lambda EMBL3 as cloning vector. Five positive clones were identified by in-situ hybridization with the full-length pGH cDNA as the probe. Dot-blot hybridization, restriction analysis, and Southern hybridization showed that one of the positive clones contained the entire pGH gene. A subclone that contained the upstream sequence from the SmaI site of pGH gene was identified, and part of it was sequenced. We also compared the sequence of our pGH gene with the corresponding sequence of the published pGH gene.

Amino Acid Sequence↗
PubMed1992

Characterization of a novel synapse-specific protein. I. Developmental expression and cellular localization of the F1-20 protein and mRNA.

A molecular description of the nerve terminal will be required to understand synaptic function fully. The goals of this study were to contribute toward such a description by characterizing a novel synapse-specific protein. A monoclonal antibody library was screened for antibodies to synaptic proteins. The antibodies were then used to isolate cDNA clones by expression screening. Here we report a detailed characterization of the protein reactive with monoclonal antibody F1-20. Immunohistochemical and biochemical analyses revealed that the F1-20 protein is synapse associated. Western blot analyses revealed that the F1-20 protein is a brain-specific polypeptide with an apparent molecular weight on SDS-PAGE of 190,000 Da. Northern blot analyses indicated that probes generated from an F1-20 cDNA clone hybridize to a single brain-specific mRNA of approximately 4.8 kilobases. In situ hybridization experiments demonstrated that F1-20 mRNA expression is neuronal specific. Northern and Western blot analyses indicated that F1-20 mRNA levels increase abruptly at postnatal day 4 and protein levels increase abruptly at postnatal day 7. This corresponds to a period of active synaptogenesis and synaptic maturation in the mouse CNS. We characterized the neuroanatomical distribution of the F1-20 protein by immunohistochemistry, and of the F1-20 mRNA by in situ hybridization. We found that the F1-20 mRNA and protein are expressed nonuniformly in brain. Variation in the expression of the F1-20 protein is complex and reveals patterns also exhibited by probes directed against other synapse-associated molecules. The highest levels of F1-20 protein are found in the cortically organized regions of the brain. The highest levels of F1-20 mRNA are found in long-distance projection neurons. There is also variation in the expression of F1-20 mRNA between different classes of large output neuron, as well as extensive variation in the expression of F1-20 mRNA between different nuclear groups.

Aging↗
PubMed1992

Characterization of a novel synapse-specific protein. II. cDNA cloning and sequence analysis of the F1-20 protein.

The F1-20 protein is a novel neuronal-specific, synapse-associated protein that is expressed nonuniformly in mouse brain. Expression of the F1-20 protein is developmentally regulated in a pattern coincident with active synaptogenesis and synaptic maturation. Here we report the cloning of the cDNA sequence for the F1-20 protein. We found two distinct isoforms of F1-20 cDNA that differed by the presence of 15 additional nucleotides, which does not interrupt the open reading frame. RNase protection analysis and PCR amplification of mouse brain RNA revealed that both isoforms are present in cellular RNA. It is likely that the two F1-20 mRNA isoforms are derived from RNA splicing events utilizing alternative 3' acceptor sites. Analysis of the deduced amino acid sequence for the complete open reading frame revealed that the predominant F1-20 mRNA encodes an 896 amino acid polypeptide with a molecular weight of 91,319 Da. The deduced amino acid sequence does not contain a signal sequence, or any extensive hydrophobic regions. The deduced amino acid sequence does contain a number of consensus sequences for protein kinases. Searches of the protein and nucleic acid sequence data bases revealed that the F1-20 protein has not been previously characterized at the primary structure level, although a weak similarity was found between rabbit calpastatin and the C-terminal portion of the F1-20 protein. We then determined biochemically that the F1-20 protein is a substrate for Ca(2+)-dependent proteolysis, which is specifically inhibited by calpain inhibitors in vitro. This indicates that the F1-20 protein is a substrate for neuronal calpain. We observed that treatment of a synaptosomal lysate with alkaline phosphatase led to an increase in the electrophoretic mobility of the F1-20 protein, as well as to an increase in the sharpness of the electrophoretic band. This indicates that the F1-20 protein is phosphorylated in vivo.

Amino Acid Sequence↗
PubMed1991

Bovine endozepine potentiates morphine analgesia in mice.

This paper describes the influence of bovine endozepine (BEP) on the analgesic effect of morphine. The intraventricular administration of BEP between doses of 2-4 nmole in mice resulted in the potentiation of the morphine-analgesic effect. The basic pain threshold did not change when BEP was given alone, thus indicating that BEP is unable to elicit analgesic effect alone. Intravenous injection of BEP in the dose range of 5-7.5 mg/kg also potentiated the analgesic effect of morphine. These observations suggest that endozepine can act as a regulator of pain.

Analgesia↗
PubMed1991

Prospective multicenter study on isolation of human immunodeficiency virus type 1 from homosexual men after seroconversion.

A prospective multicenter study was undertaken to isolate human immunodeficiency virus type 1 (HIV-1) from 45 homosexual men for a period of 30 months after seroconversion. Efficiency of HIV-1 isolation from peripheral blood mononuclear cells was relatively stable over time, ranging from 64% at the time of seroconversion to more than 82% after 18 months of seroconversion. However, Kaplan-Meier analysis of HIV-1 culture data indicates that the cumulative proportion of HIV-1 culture positivity at 3, 6, 12, and 18 months after seroconversion was 62, 65, 84, and 92%, respectively. No significant correlation was observed between the presence of HIV-1 p24 antigen in serum, or numbers of CD4+ and CD8+ blood lymphocytes, and HIV-1 isolation within this period of time. These data suggest that HIV-1 viremia in homosexual men gradually increases to almost 100% culture positivity by 18 months after seroconversion.

HIV Seropositivity↗
PubMed1991

[Synthesis of delta virus cDNA of China Sichuan strain].

delta virus cDNA of China Sichuan strain, 1.2 kb in length, was synthesized by extraction of RNA from liver tissue in a patient with subacute severe hepatitis, followed by reverse transcription and polymerase chain reaction (RT-PCR). The result was confirmed by Southern blot hybridization using 32P and biotin labelled delta virus cDNA (HN88, cloned in USA) probes respectively.

Blotting, Southern↗
PubMed1991

[Psychosocial stress and essential hypertension].

A case-control study of 72 pairs indicated that stress events in daily life stimulative factor might induce the development of hypertension in susceptible persons. The sustained stress of tension and anxiety and passive mood of psychodefence could be the psychological factors inducing hypertension. Personality, as a mediated variable, has an important influence on the initiation and progress of hypertension.

Case-Control Studies↗
PubMed1990

[Production and characterization of anti-human hepatocellular carcinoma monoclonal antibodies].

The hepatocellular carcinoma (HCC) cell line, QGY-7703, derived from a Chinese patient, was used to immunize the BALB/c mice. Fifteen hybridomas producing McAb that reacted with QGY-7703 cells were isolated from 858 hybridomas created in three cell fusions. In further studies two McAb, namely, AQGY1 and A-QGY2, were selected which specifically stained HCC cells grown in vitro. The reactivity of these McAb was not removed by the absorption by homogenates of the normal liver, but was by homogenates of HCC cells. A-QGY1 and A-QGY2 also reacted definitely with HCC cells in liver tissues of HCC patients, but neither with other cells in the tissues nor with nontransformed liver tissues of the same patients. Furthermore these two McAb stained the adult or fetal liver tissues, nor all of the other normal or tumor tissues that had been tested. Blocking and absorbing experiments revealed that A-QGY1 and AQGY2 antigens had no immunohomogenicity with antigens such as HBsAg, HBcAg, HBeAg, AFP and CEA. The specificity of these two McAb may be used potentially for the sero diagnosis, histologic identification, radioimmunoimaging and destruction of human hepatocellular carcinoma.

Antibodies, Monoclonal↗
PubMed1990

[Anastomosis of peripheral nerves by fibrin glue. An experimental study].

This paper reports the results of experimental research of anastomosing peripheral nerves by fibrinogen glue. In research of 80 sciatic nerves of 40 rats, taking epineurial suture as a control, we welded the nerves by domestic glue or Tissu-col from abroad separately to make an evaluation and survey of nerve conduct speed and gastrocnemius weight and the histology and ultrastructure. It has demonstrated that this is a more practical method for nerve repair.

Anastomosis, Surgical↗
PubMed1990

Relation of alpha and gamma interferon levels to development of AIDS in homosexual men.

Homosexual men who were human immunodeficiency virus (HIV) seropositive at enrollment into the Pittsburgh portion of the Multicenter AIDS Cohort Study had elevated titers of serum alpha and gamma interferon (IFN) within 24 months prior to development of AIDS. In contrast, subjects who developed AIDS relatively early after seroconversion to HIV during this study did not have increased levels of alpha or gamma IFN.

Acquired Immunodeficiency Syndrome↗