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Biomedical subjects

S Young

Publications and source records attributed to S Young.

At least 235 records · Page 13Linked to original sources
PubMed1988

Locally advanced adenocarcinoma of the breast without distant metastasis treated with multimodal therapies.

Locally advanced breast cancer has been treated with a variety of primary treatments with or without adjuvant therapies. This study combines radiation, chemotherapy, and surgery as a multimodal program for Stage III breast cancer. Radiation was started on day 1: 4600 rad were administered to the breast and 4500 rad were administered to the axilla and supraclavicular areas. Chemotherapy was started on day 1 with weekly intravenous injections of 5-fluorouracil (5FU) (300 mg/m2), methotrexate (15 mg/m2), vincristine (0.625 mg/m2), oral cytoxan (60 mg/m2), and prednisone (30 mg/m2 for two weeks, then 20 mg/m2 for two weeks, then 10 mg/m2 for two weeks). The 5FU, methotrexate, and cytoxan were given for 10 months postsurgery. This combination of modalities produced a complete remission in all 13 patients with Stage III breast cancer after two months of therapy. The median disease-free period was two years. The median survival was 44 months. This approach to the management of Stage III breast cancer is worthy of further investigation.

Adenocarcinoma↗
PubMed1988

Sudden death in a neonate as a result of herpes simplex infection.

This paper describes a case of a neonate with disseminated herpes simplex born to a 14-year-old asymptomatic mother. The infant's physical examination was normal at birth, and subsequent abnormalities were so subtle that infection was not recognized during life. Postmortem cultures of liver and spleen grew herpes simplex virus, and immunofluorescent direct antibody typing revealed Type 2. A cervical culture of the mother obtained after the infant's death was negative.

Adrenal Glands↗
PubMed1988

Efficacy and toxicity of tamoxifen citrate for prevention and termination of pregnancy in bitches.

Five groups of bitches were given tamoxifen citrate (1 mg/kg of body weight) orally twice daily for 10 days. Drug administration commenced during late proestrus, day 4 of estrus, day 2 of diestrus, day 15 of diestrus, or day 30 of diestrus (n = 4/group). Nineteen of the bitches accepted natural mating by 1 or more of 3 stud dogs of known fertility (1 bitch did not). Twenty days after cessation of drug administration, ovarian, uterine, and hepatic specimens were obtained from each bitch in 4 of the groups. Pregnancy proceeded to natural termination in bitches of the remaining group (diestrous day 30). Pregnancy was not detected in any bitch of the proestrus, estrous, or early diestrous groups. Of 4 bitches of each of the remaining groups (diestrous day 15 and diestrous day 30), 2 aborted fetuses and/or resorbed placental remnants; the other 2 bitches in each of these groups had normal-appearing fetuses (diestrous day-15 group) or clinically normal pups (diestrous day-30 group). Of the 20 bitches given tamoxifen citrate, 5 developed endometritis with or without pyometra, and 4 of these had ovarian cysts. Although tamoxifen citrate is effective for preventing or terminating pregnancy in the bitch, the regimen used in the study reported here was associated with high frequency of pathologic changes in the reproductive tract.

Abortion, Induced↗
PubMed1987

Down-regulation of protein kinase C is due to an increased rate of degradation.

The phorbol-ester-induced loss of protein kinase C that has been documented in many cell types appears to be a critical event in the generation of a cellular refractory state. We have investigated here the synthesis and degradation of the protein kinase C polypeptide in order to determine why its steady-state amounts are depleted in response to phorbol esters. These results indicate that depletion is due to an increased rate of degradation, with no change either in mRNA amounts or in rates of polypeptide synthesis.

Animals↗
PubMed1987

Quantitation of protein kinase C by immunoblot--expression in different cell lines and response to phorbol esters.

Antisera have been raised against human protein kinase C and also against a synthetic peptide based on the sequence of the bovine brain enzyme (LLNQEE-GEYYNVPIPE). These antibodies react with protein kinase C from a number of species (human, murine, rat, rabbit, bovine), indicating substantial conservation of epitopes. These antisera have been used to quantitate directly protein kinase C by immunoblot analysis. We show here that there is a strict correlation between the levels of immunoreactive polypeptide and extractable calcium- and phospholipid-dependent kinase activity for various cell lines. Treatment of murine, rat, and human cells with phorbol dibutyrate was found to deplete levels of immunoreactive protein kinase C severely. A detailed study of the time course of this depletion in Swiss 3T3 cells shows that it follows precisely the loss of extractable activity. On exposure to 400 nM phorbol 12,13-dibutyrate protein kinase C was essentially undetectable by 40 hours; the half-life of this down-regulation was 6.7 hours. This data thus demonstrate that the loss of immunoreactive protein kinase C and of extractable calcium- and phospholipid-dependent kinase activity precisely parallels the phorbol ester induced down-regulation of binding and responsiveness in Swiss 3T3 cells.

Animals↗
PubMed1987

Dysplastic disease of the cerebellum of an adult horse.

A 4-year-old horse was evaluated at the Colorado State University Veterinary Teaching Hospital for rapidly progressing cerebellar disease. Euthanasia was elected and at postmortem examination a proliferative mass encompassing the right side of the cerebellum was discovered. The lesion was characterized by large, convoluted, vascular folia and absence of the core of central white matter. Histologically, there was a diminution or loss of the internal granule cell layer, cavitation of the central white matter, and absence of Purkinje cells. The molecular layer was thickened with myelinated axons originating from large neurons aberrantly located in this zone. The pathology of this cerebellar mass closely resembles that seen in the cerebellum of humans with Lhermitte-Duclos disease. Mature adults are most commonly affected and clinical signs associated with this disease are often vague. The disease is thought to result from hypertrophy of granule cells possibly due to an early maturation or migration defect. Because of the late age of onset and the rarity of the disorder, its pathogenesis has been difficult to discover. Awareness that other mammalian species may be affected with a similar disorder may lead to an understanding of the causative developmental defect.

Animals↗
PubMed1987

Gaucher disease of the liver: CT appearance.

We present a child with Gaucher disease with hepatic involvement that caused portal hypertension. Computerized tomography (CT) showed distortion of liver parenchyma and central necrosis of the liver.

Ascites↗
PubMed1987

Hypertension induced by occult renal tissue.

Hypertension was noted in 2 children thought to have a solitary kidney after multiple radiological studies. Aggressive diagnostic evaluation allowed for detection of occult contralateral renal tissue. Surgical removal of this parenchyma resulted in a return to normal blood pressure.

Child↗
PubMed1987

Type I and III collagen content and fibre distribution in normal human skin during ageing.

The content of type I and III collagen in normal human skin from subjects of different ages was studied by means of a new high performance liquid chromatography method and by SDS-polyacrylamide gel electrophoresis and scanning electron microscopy. The ratio of types I and III collagen in covered skin remained constant throughout childhood and young adult life and the proportion of type III was shown to be the same as previously reported. However, in the elderly, the proportion of type III collagen in the dermis increased to a variable degree. Scanning electron microscopic examination showed a decrease in the number of collagen fibre bundles with age. Average bundle width varied significantly with age. These results may reflect an impaired synthesis of type I collagen in aged skin.

Adolescent↗
PubMed1987

Solid haemangioblastomas of the posterior fossa: radiological features and results of surgery.

Cerebellar haemangioblastomas are benign neoplasms which have been reported to have a favourable prognosis. The authors report the experience of Atkinson Morley's Hospital, London with 14 cases of solid haemangioblastoma since the introduction of the CT scan. Death or a poor result occurred in 50% of cases. Incomplete excision carried a high risk of complications. The CT scan appearances were too non specific to make an accurate preoperative diagnosis, the lesions usually being mistaken for meningiomas. Vertebral angiography can be of considerable help in identifying their vascular anatomy.

Adult↗
PubMed1987

The influence of altered tryptophan availability on ethanol intoxication in normal men.

Because of evidence that altered 5-hydroxytryptamine function may modulate ethanol intoxication, we investigated this in normal social drinkers. Using amino acid mixtures, 5-hydroxytryptamine was influenced by altering levels of its precursor tryptophan. Neither raising nor lowering tryptophan affected alcohol intoxication as measured by a self-report scale and memory and motor tasks. Our data do not support the idea that 5-hydroxytryptamine can modulate alcohol-induced intoxication.

Adolescent↗
PubMed1987

Cervical disc prolapse in the elderly: an easily overlooked, reversible cause of spinal cord compression.

It is important to distinguish cord compression due to cervical disc prolapse, which has a very favourable prognosis following surgery, from that due to cervical spondylosis. In the elderly the occurrence of cervical disc prolapse as a cause of spinal compression may be under-recognised because symptoms are too readily attributed to long standing degenerative changes on plain cervical X-rays. The difficulty of making an accurate diagnosis is complicated further in the elderly by the prevalence of other diseases which may mask the symptoms of cord compression. We report our experience with 19 patients over 60 who underwent anterior cervical discectomy for myelopathy due to intervertebral disc prolapse. There was generally a short history of walking difficulty, but the presence of 'numb clumsy hands', perhaps due to selective posterior column impairment, was often a more disabling complaint. Neck pain and disturbances of micturition were unusual. The prognosis after disc excision can be excellent. In this series all the severely disabled patients returned to an independent existence. Overall 16/19 patients made an excellent or good recovery.

Aged↗
PubMed1987

Basilar aneurysms: a new transclival approach via maxillotomy.

A transoral transclival approach to vertebrobasilar aneurysms, using a Le Fort I maxillary osteotomy rather than splitting the soft and hard palates, was employed successfully in three patients. This technique gave much improved access to the clivus, and eased exposure of the aneurysms without the need for traction on the brain stem or cranial nerves. There were no postoperative cerebrospinal fluid fistulae and no neurological complications. In one patient, a human-derived fibrin adhesive was used for dural repair. The postoperative cosmetic results were excellent and no problems relating to malocclusion were reported. This approach may have advantages when dealing with other diseases in or around the clivus.

Adult↗
PubMed1987

The role of automated ordering devices in hospital purchasing.

There are several different types of automated order devices (AODs) available to hospitals. The author of this article reviews the types of devices available, the advantages of their use for both hospital user and vendor, the documented claims as to their benefit, and disadvantages that users should be wary of in using AODs.

Computer Communication Networks↗
PubMed1987

Heterogeneous distribution of transferrin receptors on parenchymal and nonparenchymal liver cells: biochemical and morphological evidence.

To investigate which cells of the liver express the receptor for transferrin, isolated rat liver cells produced by collagenase perfusion were fractionated by repeated differential centrifugation to produce hepatocytes (95% + 1%, mean +/- SD, n = 4) and nonparenchymal cells (97% + 1%, n = 3). Saturable, high-affinity binding of 125I-transferrin was demonstrated on intact cells at 4 degrees C, with average receptor numbers 20,900 +/- 3,160 (mean + SD, n = 4) for hepatocytes and 5,500 + 1,520 (n = 3) for nonparenchymal cells. Total cellular receptors measured in detergent permeabilized hepatocytes were 42,000 +/- 18,330 (mean +/- SD, n = 3) per cell and 14,760 +/- 7,120 (n = 3) per cell in the nonparenchymal fraction. Immunocytochemical demonstration of transferrin using antitransferrin, peroxidase antiperoxidase complex confirmed that both cell types bound transferrin. There was heterogeneity of the staining reaction since there was no detectable staining on 40% of hepatocytes and 60% of nonparenchymal cells. Microdensitometric analysis of the staining product corroborated the biochemical evidence that hepatocytes have, on average, more than three times more transferrin receptors than do nonparenchymal cells. These findings support the concept that the hepatocyte has a central role in the uptake and storage of transferrin iron.

Animals↗