Search PubMed⌕ Search

Biomedical subjects

S Yoshii

Publications and source records attributed to S Yoshii.

At least 145 records · Page 8Linked to original sources
PubMed1977

Intracellular development of membrane protein of influenza virus.

The intracellular development of membrane protein (MP) of influenza A virus was investigated by immunofluorescent staining. Monospecific antiserum was prepared by immunizing rabbits with MP eluted from SDS-polyacrylamide gels of SDS-disrupted NWS virions. In the productive infection in clone 1-5C-4 cells, MP antigen was first detected over the whole cell at 4 hr after infection, concomitantly with the appearance of hemagglutinin (HA) antigen in the cytoplasm, and bright nuclear fluorescence was then observed. Nucleoprotein (NP) antigen was detected in the nucleus prior to the appearance of fluorescence of MP antigen and thereafter the cytoplasmic fluorescence developed. Late in infection, all of these three antigens were observed predominantly in the cytoplasm with stronger fluorescence at the cell surface. Essentially similar findings were obtained in the abortive infections in L cells and BHK cells. The above results suggest that the membrane protein of influenza A virus is present in the nucleus as well as in the cytoplasm of infected cells.

Cell Line↗
PubMed1975

Modification of normal cell surface by smooth membrane preparations from BHK-21 cells infected with Newcastle disease virus.

Smooth membrane fractions were prepared from the cytoplasmic extract of BHK-21 cells infected with Newcastle disease virus (NDV). These membranes exhibited high hemagglutinating, neuraminidase, and hemolytic activity but little infectivity, suggesting that they might be precursors for viral envelope. When such membranes were adsorbed to the monolayers of uninfected BHK-21 cells at 4 degrees C and then incubated at elevated temperature for a couple of hours, the cells became highly hemadsorptive even in the presence of cycloheximide. This phenomenon occurred between 15 degrees C and 25 degrees C, and was maximal at 31 degrees C, where approximately 4 times more erythrocytes were adsorbed than to the cells incubated at 4 degrees C. Immunofluorescent staining suggested that diffusion of viral antigens might occurred rapidly over the entire surface of the cells. Cell fractions containing virions induced hemadsorption in uninfected cells, too. However, induction occurred now at 31 degrees C and was maximal at 37 degrees C, and erythrocytes appeared to be adsorbed not to the entire surface of the monolayer but restricted areas of the cells. The diffusion of viral antigens on the cell surface was not so significant under these conditions. On the basis of these findings the possible role of the membranes of the smooth endoplasmic reticulum in virus replication is discussed.

Antigens, Viral↗
PubMedDate not supplied

Comparison of predictive value for colorectal cancer in subjects with and without rectal bleeding.

BACKGROUND/AIMS: To clarify the association between a sign of rectal bleeding and colorectal cancer, and to reveal the relationship of rectal bleeding to the results of an immunochemical fecal occult blood test. METHODOLOGY: In a population-based cross sectional study, 30,138 subjects who received immunochemical fecal occult blood screening with a 2-day method were divided into two groups, according to the results of a questionnaire on a sign of rectal bleeding, and the positivity rate of an immunochemical occult blood test as well as the predictive value for colorectal cancer were compared in the two groups. RESULTS: The fecal occult blood test was positive in 8.8% of subjects with rectal bleeding and in 6.0% of subjects without rectal bleeding, and the predictive value was 6.4% and 3.3% in subjects with and without rectal bleeding, respectively, showing a significant difference in the positivity rate (p<0.001) as well as the predictive value (p<0.05) between these two groups. CONCLUSIONS: These findings indicate that there are positive relations between the subjects with rectal bleeding presentation and colorectal cancer, and a sign of rectal bleeding and the results of an immunochemical fecal occult blood test.

Colorectal Neoplasms↗
PubMedDate not supplied

Repair of coarctation with persistent fifth arterial arch and atresia of the fourth aortic arch.

Coarctation of the aorta with persistent fifth arterial arch and atresia of the fourth aortic arch between the left common carotid and left subclavian arteries was treated surgically in a two-month-old boy with transposition of the great arteries and double-outlet right ventricle. The aortic arch was repaired using side-to-side anastomosis of the left common carotid and left subclavian arteries, patch repair of the coarctated segment at the origin of the left subclavian artery, and ligation of the patent ductus arteriosus. Pulmonary arterial banding and balloon atrioseptostomy were performed for associated anomalies.

Anastomosis, Surgical↗