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Biomedical subjects

S Yang

Publications and source records attributed to S Yang.

At least 397 records · Page 22Linked to original sources
PubMed1997

[Determination of Pb in flying coal-ash by GTAAS].

Biochemical was chosen as the stability and improvement reagent to make insoluable solid into slurry, which could be sampled directly to determine Ph in flying coal-ash, one of the environmental samples. The optimal conditions of Hitichi GTAAS 180-80 were decided by experiments, such as linear range 7.0-66.7ng/mL atomization temperature 2000 degrees C and amount of biochemical 0.2-1.2 per thousand. The effects of acid, stirring and particle concentration were also addressed.

English Abstract↗
PubMed1997

Effect of melittin on the apical membrane Na+ and Cl- conductances of frog cornea epithelium.

The transepithelial conductance increased with 10(-6) M melittin on the tear side of the frog cornea. This effect was attributed to the opening of an apical membrane Na+ conductance. However, the potency of the venom at this concentration (apical membrane fractional resistance (fRo) decreased to near zero) masked this and other effects. With 3 x 10(-7) M on the tear side, the effects were finite (fRo > 0) and reversible. With fRo > 0, the effects of melittin could be readily studied on the transport parameters of Na+, Cl-, and K+. Epithelial cells of intact bullfrog corneas were impaled with microelectrodes using an in vitro preparation. Under short-circuit current (Isc) conditions, 20 min after melittin Isc increased by 3.1 from 8.2 microA/cm2., fRo decreased by 18 from 51%; the intracellular potential, Vo, depolarized by 19.4 from -56.5 mV; and the transepithelial conductance, gv increased by 0.57 from 0.29 mS/cm2. Tenfold decreases in tear Na+ or Cl- concentrations changed the transport parameters consistent with the formation of a Na+ conductance and an increase in the apical membrane Cl- conductance by the venom. These conclusions were further supported by the minimal effect of melittin in Cl(-)-fre and, particularly, in Na(+)-free solutions. Changes in K+ concentration had no effect on transport parameters. These findings indicate that the effect of melittin at this low concentration is upon a Na+ channel protein and not due to nonspecific conductances.

Animals↗
PubMed1997

Antagonistic effect of human alpha-1-antitrypsin on excystation of Cryptosporidium parvum oocysts.

This study evaluated the effects of the human serine protease inhibitor alpha-1-antitrypsin (AAT) on in vitro excystation and infectivity of Cryptosporidium parvum. Excystation was monitored at 37 C in RPMI medium in the presence of 0, 100, 500, or 1,000 micrograms/ml AAT. AAT significantly inhibited (P < 0.05) excystation of bleach-decontaminated oocysts in a concentration-dependent manner at incubation intervals from 15 to 90 min but did not alter the excystation dynamics of unbleached oocysts. Bleach-treated oocysts, suspended in RPMI containing 0, 1, 10, 100, 500, or 1,000 micrograms/ml AAT, were used to inoculate bovine fallopian tube epithelial (BFTE) cell monolayers. Alternately, sporozoites, excysted at 37 degrees C and collected by filtration, were used to inoculate BFTE cells under the same conditions. The mean number of parasites counted in AAT-treated, oocyst-inoculated cells was significantly less (P < 0.01) than control mean values at 24 and 48 hr post-inoculation (PI); longer PI intervals (72-96 hr) exhibited a decreased inhibitory effect. AAT did not inhibit parasite infection when cultures were inoculated with C. parvum sporozoites. The findings of this study show that the anticryptosporidial potential of AAT is primarily associated with an antagonistic effect on oocyst excystation.

Animals↗
PubMed1997

Angiocentric T cell lymphoma of the skin presenting as inflammatory nodules of the leg.

We describe two cases of malignant lymphoma presenting as inflammatory nodules of the leg and mimicking panniculitis clinically. In both cases the skin biopsies showed prominent involvement of the subcutaneous tissue by lymphoma cells. In addition, lymphoma cells invaded blood vessels in the dermis or the subcutaneous tissue. One case was characterized by predominantly extravascular and intravascular location of the lymphoma cells, and the other case by the predominantly extravascular and intramural location of lymphoma cells. These histological findings were compatible with those of angiocentric T-cell lymphoma but with some unusual features. Angiocentric T-cell lymphoma of the skin should be listed in the group of diseases which present as inflammatory nodules of the leg.

Adult↗
PubMed1997

[Improving the repair of the skull defects in 60 children].

The skull defects with neogenetic bone were repaired with a method in which the neogenetic bone, the cranial periosteum were sutured and fixed to a Ti-net. Totally 60 children were treated. This method has following advantages: 1. Averting trephining of skull to reduce blood loss and the possibility of brain injury: 2. The neogenetic bone is well protected so that it would grow more normally; 3. The method is easy and the operation time is shortened; 4. There is little reaction and postoperative results are satisfactory. The paper describes the principle and technic of the method.

Brain Injuries↗
PubMed1997

[The measurement of ST-T segment parameters in ambulatory ECG].

The measure methods of ECG parameters in the repolarization process were studied experimently. The conception of adaptive adjustment in ST segment level measurement was proposed, and a scheme for the adjustment was established with clinical experiences. With the progress of ambulatory ECG, the sectioned and equivalent areas of ST-T segments were extracted.

Electrocardiography, Ambulatory↗
PubMed1996

Crystal structure of an IHF-DNA complex: a protein-induced DNA U-turn.

Integration host factor (IHF) is a small heterodimeric protein that specifically binds to DNA and functions as an architectural factor in many cellular processes in prokaryotes. Here, we report the crystal structure of IHF complexed with 35 bp of DNA. The DNA is wrapped around the protein and bent by >160 degrees, thus reversing the direction of the helix axis within a very short distance. Much of the bending occurs at two large kinks where the base stacking is interrupted by intercalation of a proline residue. IHF contacts the DNA exclusively via the phosphodiester backbone and the minor groove and relies heavily on indirect readout to recognize its binding sequence. One such readout involves a six-base A tract, providing evidence for the importance of a narrow minor groove.

Amino Acid Sequence↗
PubMed1996

IL-4 is protective against development of toxoplasmic encephalitis.

IFN-gamma is critical for prevention of development of toxoplasmic encephalitis (TE). Since IL-4 down-regulates production of IFN-gamma, we examined its role in the pathogenesis of TE in IL-4-targeted mutant (IL-4-/-) mice. IL-4-/- mice all died from 6 to 20 wk after peroral infection with cysts of the ME49 strain of Toxoplasma gondii; control mice survived. At 4 and 8 wk after infection, significantly greater numbers of T. gondii cysts and foci of acute inflammation, and greater amounts of tachyzoite-specific mRNA (by reverse-transcriptase PCR) were in brains of IL-4-/- mice than controls. Toxoplasma IgG2b and IgG3 Ab levels were slightly but significantly higher in sera of IL-4-/- than control mice, whereas IgM and IgG2a levels did not differ between these mice. Toxoplasma IgG1 and IgE Abs were not detected in sera of either strain. Amounts of IFN-gamma, TNF-alpha, IL-6, and IL-10 mRNA detected by reverse-transcriptase PCR did not differ between brains of infected IL-4-/- and controls, although brains of the former mice had greater numbers of inflammatory mononuclear cell infiltrates. IL-4 mRNA was detected only in infected control mice. Spleen cells of control mice at 8 wk after infection produced significantly greater amounts of IFN-gamma following stimulation in vitro with soluble T. gondii Ags than did those from IL-4-/- mice. These results indicate that IL-4 is protective against development of TE by preventing formation of T. gondii cysts and proliferation of tachyzoites in the brain. The impaired ability of IL-4-/- mice in the late stage of T. gondii infection to produce IFN-gamma most likely contributes to their susceptibility for development of severe TE.

Animals↗
PubMed1996

Salutary and deleterious effects of acidity on an indirect measure of metabolic rate and ATP concentrations in CNS cultures.

Acidosis has traditionally been considered to mediate certain types of hypoxic-ischemic injury to the brain. However, the recent demonstration that moderate acidosis will reduce NMDA-mediated currents suggested that acidity could actually protect against types of ischemia and excitotoxicity, and in vitro studies now support this idea. Prompted by this, we have utilized the silicon microphysiometer, a recently-developed instrument that allows for indirect real-time measurement of metabolic rate by detecting proton efflux from small numbers of cultured cells, to determine whether acidity has protective effects upon cellular metabolism. Reducing extracellular pH from 7.4 to as low as 6.0 caused prompt, step-wise, and reversible inhibition of proton efflux rate in cortical and hippocampal cultures both normally and restricted to either glycolysis or oxidative metabolism. Approximately half of the inhibition was due to acidotic effects of NMDA-mediated currents, as demonstrated with NMDA receptor antagonists. Such an inhibition of this indirect metabolic measure could be associated with constant or increased ATP concentrations and represent a beneficial decrease in energy demands upon a neuron. Alternatively, an inhibition of proton efflux rate could be associated with ATP depletion and reflect impaired energy production. We observed a complex interplay between these opposing patterns. Reducing pH to 6.7 for 20 min caused significantly increased ATP concentrations, and prevented excitotoxin-induced ATP depletion. These effects of acidosis involved both NMDA-dependent and- independent actions. More severe (less than pH 6.7) acidosis did not cause ATP concentrations to rise, and if sustained for more than an hour caused a significant decline in ATP concentrations. Thus, despite the recent emphasis on the surprising neuroprotective potential of acidosis, a drop in pH is still likely to have complex and mixed consequences for brain tissue.

Acidosis↗
PubMed1996

Chemical selectivity in micellar electrokinetic chromatography. II. Rationalization of elution patterns in different surfactant systems.

Retention behavior in micellar electrokinetic chromatography (MEKC) is investigated using linear solvation energy relationships (LSERs) for two pseudo-stationary phases, one consisting of cationic micelles of tetradecyltrimethylammonium bromide (C14TAB) and the other of an anionic triblock copolymer, poly(methyl methacrylate-ethyl acrylate-methacrylic acid) (Elvacite 2669). It was found that solutes' migration behaviors in these two MEKC systems are mainly influenced by their size (V/100) and hydrogen bonding acceptor (HBA) strength (beta). However, solutes' hydrogen bonding donor (HBD) strength (alpha) has minor effects on their migration in MEKC. The characteristics of these two systems were compared to three other previously reported anionic micellar systems of sodium dodecyl sulfate (SDS) (anionic hydrocarbon), sodium cholate (SC) (anionic bile salt) and lithium perfluorooctane sulfonate (LiPFOS) (anionic fluorocarbon). It was concluded that hydrogen bonding interactions play a major role in providing different chemical selectivity among these five MEKC systems. Both C14TAB micelles and the ionic polymer of Elvacite 2669 provide hydrogen bonding acceptor (HBA) sites for solutes, which is similar to SC micelles. In fact, C14TAB is the strongest HBA, while Elvacite 2669 has HBA strength similar to that of SC micelles. On the other hand, the fluorocarbon micelles of LiPFOS are the strongest hydrogen bond donor (HBD) micelles, followed by the weak HBD SDS micelles. In general, cavity formation has little or no effect on chemical selectivity among hydrocarbon surfactant MEKC systems (i.e., SDS, SC and C14TAB). Information obtained from the LSER analysis is used to rationalize the elution patterns in MEKC with different types of pseudo-stationary phases.

Chromatography, Liquid↗
PubMed1996

FTIR studies of recombinant human granulocyte-macrophage colony-stimulating factor in aqueous solutions: secondary structure, disulfide reduction and thermal behavior.

Fourier transform infrared spectroscopy (FTIR) has been used to investigate the secondary structure, disulfide reduction and thermal behavior of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) in aqueous solutions. The contributions of amino-acid side-chain groups to the amide I bands of rhGM-CSF in H2O and in D2O solutions were carefully scrutinized, as 40% of the total 127 amino-acid residues of rhGM-CSF is side-chain absorptive (asparagine, glutamine, etc.). The FTIR results indicated that rhGM-CSF is composed of 46% alpha-helix, 7% beta-sheet, 23% turn and 24% loop/irregular structures which are in good agreement with the X-ray diffractional data. Reduction of rhGM-CSF with dithiothreitol caused apparent unfolding of the native conformation followed by the time-dependent increase of beta-aggregation bands which arose at 1622 and 1693 cm(-1) in H2O, 1613 and 1684 cm(-1) in D2O solutions. The result also showed that tertiary structure can change independently of the secondary structure. Thermal denaturation of rhGM-CSF took place at 55 to 70 degrees C and the denatured protein adopted an irregular structure as revealed by the FTIR spectra. The thermal denaturation did not show the formation of intermolecular beta-aggregates which is typical of most thermal denatured proteins. Moreover, it is partly reversible, indicating a special thermal stability of rhGM-CSF.

Deuterium Oxide↗
PubMed1996

Impact of plasmid presence and induction on cellular responses in fed batch cultures of Escherichia coli.

Fed batch cultivations of plasmid-free and recombinant Escherichia coli were employed in order to determine cellular responses and effects of plasmid presence and induction on the host cell physiology. While plasmid presence was shown to have minor influence on overall biomass yield, induction with 0.1 mM IPTG led to a marked reduction. The number of dividing cells, measured as colony forming ability, was influenced by plasmid presence and to a larger extent by induction. The latter caused a decline in the number of dividing cells to less than 10% of the population within 10 h. However, this cell segregation did not affect the specific rate of product formation, which was approximately constant throughout the cultivations. Analysis of the in vivo degradation rate of the product indicated that it was proteolytically stable. The cellular content of the stringent response signal substance, ppGpp, peaked immediately after transition from batch to fed batch mode to stabilise at a higher value than in the batch phase. When the specific growth rate declined below 0.06 h-1 an additional rise in ppGpp concentration was observed.

Cell Division↗