Evidence for neutrophil involvement in the development of subclinical emphysema
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Journal Article↗
Biomedical subjects
S Xu
Publications and source records attributed to S Xu.
Chronic toxicity of rare-earth elements on human beings: implications of blood biochemical indices in REE-high regions, South Jiangxi.
Blood analyses for rare-earth element (REE)-high background regions in South Jiangxi show that the population averages of many of the biochemical indices deviate markedly from normal values in the normal region. These deviations are thought to be caused by prolonged intake of REE through food chains in view of that the toxicity of other harmful metals such as Pb and Cd can be neglected because of their insignificant amounts in the environment. In comparison with the normal region, blood biochemical indices abnormal in the REE-high regions are manifested as low total serum protein (TSP), albumin, beta-globulin, glutamic pyruvic transitanase, serium triglycerides, and immunoglobulin, but high cholesterol. These deviations may be related to the REE concentration and composition of food chains, and are sex dependent. Certain blood indices (such as TSP) of different age groups in the LREE-high region indicate that the influence of REE on males is a one-way irreversible process, whereas females show a strong ability of restoration.
Adult↗
Containment of Helicobacter hepaticus by use of husbandry practices.
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Animal Husbandry↗
New potential photodynamic therapeutic anti-cancer agents: synthesis and characterization of demethoxy amino-substituted hypocrellins.
A novel method was used to obtain demethoxy amino-substituted hypocrellin derivatives. The reaction condition was mild and amino substitution occurred at position 2 or 11 of hypocrellins with high yield. The photophysical and photochemical properties of the amino-substituted hypocrellins derivatives were investigated, and their significantly enhanced red absorptivities and strong active oxygen-generating functions qualified them as promising photodynamic therapeutic anti-cancer agents.
Antineoplastic Agents↗
[Study on type I and type III procollagen mRNA and in situ collagen proteins expressions in hypertrophic scars after intralesional Kenalog treatment].
OBJECTIVE: This is to investigate the mechanism of effects of Kenalog on type I and III collagen syntheses and degradation in hypertrophic scars. METHODS: Intralesional injection of Kenalog was performed on 6 patients with hypertrophic scars. On the 3rd day and 7th day after the treatment, 6 samples from the 6 patients each were collected and type I and III collagen proteins and in situ procollagen mRNA expressions were studied by means of immunohistochemistry and molecular biology. RESULTS: On the 7th day after intralesional injection of Kenalog, type I collagen proteins reduced (P < 0.05) and type III collagen proteins did not reduced significantly (P > 0.05). On the 3rd day after intralesional injection of Kenalog, both type I and type III procollagen mRNA expressions were significantly inhibited and on the 7th day both procollagen mRNA expressions decreased further. CONCLUSIONS: Intralesional Kenalog may inhibit type I procollagen mRNA expression more than type III. Gene expressive intensions of type I and III procollagen were higher in hypertrophic scars than in normal skin.
Adolescent↗
[Rapid rifampicin susceptibility test by using recombinant mycobacteriophages].
OBJECTIVE: To set up a fast, sensitive and specific way to detect mycobacteria and rifampicin susceptibility to mycobacteria by using recombinant mycobacteriophages and bioluminescent method. METHODS: Firstly detected the luciferase activity in different bacteria by using mycobacteriophages, then assessed the best drug concentration of rifampicin for drug susceptibility test in luciferase reporter assay, and lastly used the above conditions to decide rifampicin susceptibilities to different mycobacteria and compare the result with L-J medium culture. RESULTS: Bacteriophages had high light production specifically in mycobacteria and only very low light production in E. coli, the difference being obvious. The light production of rifampicin-resisitant mycobacterium strains was much higher than that of sensitive strains in culture with rifampicin(P < 0.05). Different drug concentrations of rifampicin were used to optimize drug concentration for rifampicin drug susceptibility test and the optimum concentration was found to be 2 micrograms/ml. The correlation of drug susceptibility test between luciferase reporter phages to L-J medium in standard strains and clinical isolates was the sarce. Temperature sensitive Phage 88 was more sensitive than Phage 40(P < 0.05). The time for drug resistance test was 72 hours. CONCLUSIONS: Luciferase reporter phage can detect mycobacteria specifically and can be used in rifampicin susceptibility test. This assay is a fast, sensitive and specific method to detect mycobacterium strains and their resistance to rifampicin.
Antibiotics, Antitubercular↗
[Clinical research of Hashimoto's disease with thyroid carcinoma].
OBJECTIVE: To study the relation diagnosis and treatment of Hashimoto's disease (HD) with thyroid carcinoma (TC). METHODS: The relation diagnosis and surgical treatment of 150 cases of HD including 15 cases of TC were analyzed. RESULTS: The coexistent rate of HD with TC was 10%. The 15 cases were all women, with a mean age of 36.5 years, and they were surgically treated. Follow-up for 4 months to 19 years showed that the 15 cases were alive without recurrence. CONCLUSION: Surgical treatment is indicated for patients with HD associated with TC. The essential factors for diagnosis and rational treatment are a detailed understanding of clinical feature, routine examination of serum antibodies, B-ultrasonic examination and selective FNAC.
Adult↗
[Mechanism of abnormal scars with treatment of steroid].
OBJECTIVE: To investigate the mechanism of steroid in treatment of abnormal scars. METHODS: Apoptosis of different fibroblasts from 6 samples with keloid, 6 samples with hypertrophic scar, and 6 samples of normal skin was observed under the condition of the media containing steroid in vitro. Proliferation, biosynthesis and apoptosis of fibroblasts of 6 samples of hypertrophic scars treated with intralesional injection of steroid were studied in vivo. RESULTS: Steroid could induce apoptosis of different fibroblasts in vitro in correspondence with increasing ratio of Bax/Bcl-2 proteins. Intralesional injection of steroid could inhibit proliferation of fibroblasts from hypertrophic scars by inhibiting PDGF-BB gene expression in vivo. Intralesional injection of steroid could inhibit procollagen gene expression to prohibit type I and III protein syntheses of fibroblasts from hypertrophic scars in vivo by inhibiting gene transcription. Intralesional injection of steroid could increase c-myc and p53 gene expression of hypertrophic scars in vivo, which induced apoptosis of cells. CONCLUSION: The effects of steroid on abnormal scars were achieved by inhibiting proliferation and biosyntheses of fibroblasts and promoting apoptosis of fibroblasts.
Adolescent↗
[Measurement of tumor angiogenesis in primary lung cancer].
OBJECTIVE: To study tumor angiogenesis in primary lung cancer and its relation with lymph node metastasis, tumor cell apoptosis, and cell proliferation. METHODS: Intratumor microvessel was detected by factor VIII polyclonal antibody using immunohistochemistry. Tumor cell apoptosis was detected by TUNEL method. Cell proliferation was analyzed by flow cytometry. RESULTS: Intratumor microvessel density (MD) was significantly lower in stage I lung cancer than in stage II and stage III (P < 0.01). MD was significantly higher in the tumors with lymph node metastasis (P < 0.01). With the increase in MD, there would be a 1.2-fold increase in the risk of lymph node metastasis (P < 0.01). With the increase of MD, apoptosis index (AI) of the tumor significantly decreased, but no relationship between MD and cell proliferation index (PI) was observed. CONCLUSION: Tumor angiogenesis promotes growth and metastasis of lung cancer. Tumor angiogenesis also can inhibit the apoptosis of lung cancer.
Adult↗
[Studies on gene mutation and micronucleus formation induced by ethylnitrosourea (ENU) in transgenic mice in vivo].
OBJECTIVE: To determine the feasibility of using pUC 118NX plasmid-based transgenic mice in the study of induced mutation in vivo with known mutagen, ethylnitrosourea (ENU). METHODS: pUC 118NX plasmid was recovered from the spleen genomic DNA of ENU-treated and untreated xylE, C57BL/6J transgenic mice with enzyme digestion and circularizing. The recovered pUC 118NX plasmid was electroporated into DH10B host cells, which were incubated in LB solid medium containing proper ampicillin overnight and then sprayed with catechol solution. Mutant could be detected by difference in yellow and white color of the stains and its xylE target gene could be sequenced for further identifying its mutation type. In addition, peripheral blood and bone marrow cells were isolated from xylE, C57BL/6J transgenic mice and micronucleus frequency (MNF) induced by ENU was observed. RESULTS: The spontaneous mutant frequency for xylE gene in the spleen of xylE, C57BL/6J transgenic mice was less than 4.79 x 10(-5), significantly different from that treated with ENU (50 mg/kg), 19.83 x 10(-5). Types of gene mutation induced by ENU in the spleen of xylE, C57BL/6J transgenic mice included transversion (50%), one or two-base insertion (37.5%) and transition (12.5%). MNF in peripheral blood normochromatic erythrocyte (NCE) and in bone marrow polychromatic erythrocyte (PCE) of ENU-treated (50 mg/kg x 5) mice were 7.6 per thousand and 8.8 per thousand, respectively, both significantly different from the controls treated with solvent (P < 0.01). It indicated that chromosome aberration could be induced by ENU. CONCLUSION: The pUC118NX plasmid-based xylE, C57BL/6J transgenic mice, which could be used in detecting gene mutation and chromosome aberration in vivo simultaneously, provided a novel detection system for overall assessment of genetic toxicity of chemicals.
Animals↗
[Direct effects of fluoride on activities of bone morphogenetic protein].
OBJECTIVE: To demonstrate the direct effects of fluoride on activities of bone morphogenetic protein (BMP). METHOD: A complex sample containing BMP and sodium fluoride was implanted under the abdominal skin of young male Wistar rats. The histological characteristics, and activities of alkaline phosphatase and acid phosphatase of the implanted were examined to study the effects of fluoride on biological activities of BMP. RESULTS: More fibroblasts cell were transformed to osteoblasts cell in animals treated by BMP with fluoride than those treated by BMP only. During bone formation induced by BMP, fluoride increased the activities of osteoblasts and accelerate mineralization, while inhibited the activities of osteoclast. CONCLUSION: Fluoride can affect biological activities of BMP directly.
Acid Phosphatase↗
[Exploration of gene expression profiles of CD(34)(+) hematopoietic stem/progenitor cells based on large scale sequencing].
OBJECTIVE: To set up a large scale sequencing system and explore the gene expression profiles of CD(34)(+) hematopoietic stem/progenitor cells (HSPCs). METHODS: CD(34)(+) cells were isolated from umbilical cord blood and subjected to cDNA library construction. A primary profile of gene expression in HSPCs was emerged by EST sequencing and bioinformatics analyzing. RESULTS: Among 9,866 ESTs thus obtained, 7 476 meaningful ESTs were clustered into 2,060 unique sequence species (USSs), representing 1,054 known gene species and 1,006 unknown gene fragments. The 1054 known genes were divided into 8 categories: (1) hematopoiesis associated: 73, (2) chromatin structure and cell division/apoptosis: 91, (3) signal transduction and receptors: 111, (4) cell structure/mobility: 48, (5) cell/organism defense/homeostasis: 41; (6) Gene expression (transcription, translation and modification): 265, (7) metabolism: 192; and (8) unclassified: 233. CONCLUSION: A gene expression profile including 1,054 known genes and 1006 new gene fragments of HSPCs was primarily obtained, which may lay a basis for the further study on the molecular mechanism of hematopoiesis regulation and provide candidates for new gene cloning.
Antigens, CD34↗
[The preliminary study on the gene mutations of sixty patients with G6PD deficiency in Yunnan province].
OBJECTIVE: To analyze the types and distribution of glucose-6-phosphate dehydrogenase (G6PD) gene mutations in Yunnan province. METHODS: By using the natural primers or mis-matched primers mediated polymerase chain reaction (PCR) followed by endonuclease digestion methods, six types of G6PD gene mutations, G1388A, G1376T, C1024T, G392T, C592T and A95G were in sixty patients with G6PD deficiency. RESULT: Six types of mutation were revealed: 28.3% (17 of 60) were G1388A, 11.7% (7 of 60) G1376T, 6.7% (4 of 60) C1024T, 1.7% (1 of 60) G392T, 1.7% (1 of 60) C592T, 1.7% (1 of 60) A95G and 48.3% (29 of 60) unidentified. C592T mutation is the first report in Yunnan people. CONCLUSIONS: The G6PD gene in Yunnan people is heterogeneous. Research of G6PD gene mutation types may provide some useful data for clinical diagnosis and prevention of G6PD deficiency, and for human genetic study.
Amino Acid Substitution↗
[Role of calcineurin in cardiac fibroblast proliferation stimulated by angiotensin II].
The present study was undertaken to observe the role of calcineurin (CaN) in the angiotensin II (Ang II) stimulated cardiac fibroblast proliferation. In cultured cardiac fibroblasts of neonatal rats, Ang II was used to stimulate proliferation while CaN was inhibited by CaN CsA inhibitor cyclosporin A (CsA). (3)H-TdR incorporation, activity of CaN and intracellular calcium concentration were measured. (3)H-TdR incorporation of Ang II stimulated fibroblasts was 72% higher than control (P<0.01), which was inhibited by CsA (0.1 10 micromol/L) in a dose dependent manner. Intracellular Ca(2+) level and CaN activity of Ang II stimulated fibroblasts were respectively 112% (P<0.01) and 17%(P<0.05) higher than control. It is concluded that CaN may play an important role in signal transduction of the Ang II induced cardiac fibroblast proliferation.
Angiotensin II↗
[Effects of homoharringtonine on proliferation of human retro-ocular fibroblasts].
This is a study amied at the effects of homoharringtonine on the proliferation of human retro-ocular fibroblasts. The human retroocular fibroblasts (HROFb) were cultured and MTT colorimetry assay was used to determine the proliferation of HROFb. The influence of homoharringtonine on the proliferation of HROFb was observed in vitro. The results showed that MTT colorimetry assay could precisely reflect the proliferation of HROFb. The absorbance was directly proportional to the number of the cells (r = 0.996, P < 0.001). The proliferation of HROFb was inhibited by the homoharringtonine and the cell proliferation percentage was correlated positively to the homoharringtonine level (r = 0.6932, P < 0.001).
Cell Division↗
[Effects of bacterial protective liquid on radiation biology effect of mouse bone marrow cells].
To study the relationship between bacterial protective liquid (BPL) and the biological effect of mouse bone marrow cells by gamma-ray irradiation, apoptosis and the number of CD4+, CD8+ and NK cells were analyzed by flow cytometry(FCM), the results showed that BPL decreased the apoptosis and increased the proliferation of cells(P < 0.01). The theorem of action was related with raising the percentage vitality of NK cells and the quantity of CD4+ and CD8+. This study proved that BPL might have protective effect against radiation injury.
Animals↗
[The effects of starfish sterol on platelet aggregation].
Through the internal and external experiments of platelet aggregation on rats and rabbits, we study the effects of starfish sterol (No. A1998) on the formation of experimental thrombus in vitro and the effects of platelet aggregation induced by arachidonic acid (AA), ADP, CaCl2. Starfish sterol A 1998 can inhibit the formation of experimental thrombus in vitro of rats on the rates of 10.0%, 20.1%, 25.9% (P < 0.01), p.o. starfish sterol 4.5, 13.5, 40.5 mg/kg. A 1998 can inhibit platelet aggregation induced by AA, ADP, CaCl2 in the same time. It can be concluded that starfish sterol has inhibitory effects on platelet aggregation.
Adenosine Diphosphate↗
[Pharmacological studies of trilex on treatment of pharyngitis].
Trilex consisted of three species: Ilex latifolia, Ilex asprella and Ilex rotunda. Trilex had the antipyretic effects, decreased the rat body temperature about 0.8 degree C on yeast induced hyperthermia. Trilex could obviously enhance the threshold of hot plate induced pain in mice. The maximum threshold increased 43.8%. Trilex could also inhibit acetic acid induced inflammatory ooze in mice abdominal cavity. The inhibitory rate was 34.8%. These results showed that trilex had antipyretic, analgesic and anti-inflammatory effects. It could be used to treat acute and chromic pharyngitis.
Analgesics↗