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Biomedical subjects

S Watabe

Publications and source records attributed to S Watabe.

177 records · Page 10Linked to original sources
PubMed1975

Postoperative bronchopleural fistula: clinical and experimental study.

It is a well-known fact that in pulmonary tuberculosis patients treated by resection, the quality of the suture material used for closing the bronchial stump plays an important role in the pathogenesis of postoperative bronchopleural fistula. Of 426 cases treated surgically and in whom silk suture thread was used, 23 developed bronchopleural fistula, whereas none of the 220 cases sutured with nylon monofilament developed abnormality. Statistical analysis of 100 surgical cases with silk thread suture and of 100 cases with nylon monofilament suture showed that the two groups had no marked differences as to background factors. Howevers, as compared with the silk-thread suture group, the nylon-monofilament suture group revealed more consistently favorable postoperative bronchoscopic findings. Experimental studies with dogs showed a similar lack of complications when the monofilament suture material was used, as contrasted were conducted in hospital by the same surgical personnel using the same procedures, it can be said that, to insure prevention of complications, the suture material for bronchial stump closure should be of non-irritating nature and preferably of monofilament strength and quality, such as nylon monofilament.

Animals↗
PubMed1975

Lympho-reticuloendothelial activity of early gastric carcinoma.

The lympho-reticuloendothelial activities of 54 cases of early gastric carcinoma were studied. Morphological blastoid transformation of lymphocyte by the addition of phytohemagglutinin-P (PHA-P) was disturbed in half of the patients. The DNA synthesizing activity of lymphocyte was not stimulated by PHA-P in the cases of advanced carcinoma but it was stimulated in the cases of early carcinoma. The tuberculin reaction was negative in 30% of the cases of early carcinoma. The reticuloendothelial system (RES) activity studied through the congo red method revealed that 86% of the cases of early carcinoma had disturbed RES function.

Adolescent↗
PubMedDate not supplied

ATP-dependent protease in human placenta.

We have found that human placental mitochondria contain ATP-dependent protease in a soluble form. The molecular weight of the protease have been up to 108,000, which is the same as ATP-dependent protease in bovine adrenal cortex. Since this protease has been distributed among steroid hormone-producing tissues such as testis and adrenal cortex and ATP-dependent protease can degrade cytochrome P-450scc, a key enzyme in steroid hormone biosynthesis, we suggest that the protease may have an important role in the regulation of steroid hormone metabolism.

ATP-Dependent Proteases↗
PubMedDate not supplied

Feasibility of measuring 5-fluorouracil catabolic potential by oral loading.

The efficacy of 5-fluorouracil (5-FU) treatment and the incidence of adverse events differ among patients and depend to some extent on individual variations in drug catabolism. This feasibility study aimed to determine the optimum conditions for a 5-FU oral load test, which would allow the simple evaluation of individual differences in 5-FU catabolism. Patients with colon cancer were given oral 5-FU (200 mg/day) for 3 days (n = 36) or a single 100 mg dose (n = 14). Serum concentrations of uracil, dihydrouracil, 5-FU and 5-fluoro-5,6-dihydrouracil were measured before and after 5-FU administration. The results suggested that a decline in 5-FU metabolism was associated with continuous administration and increasing age. We conclude that a continuous load of 5-FU is necessary in order to predict the efficacy and side-effects of the drug. The 3-day regimen, with its ease of administration, merits further study to assess its possible clinical application.

Administration, Oral↗
PubMedDate not supplied

[Activity of ATP-dependent protease and steroid metabolism in the human placenta].

Placenta play the important role of nourishment of fetus and for the regulation of fetal and maternal steroid metabolism. Steroid metabolism is synthesized in mitochondria and levels of enzymes in steroid metabolism are regulated by the rate of synthesis and degradation of these enzymes. Therefore, we studied protease in human placental mitochondria to clarify regulation mechanism of steroid-synthesizing enzymes. 50 micrograms of human early and term placenta homogenate, proteins were analyzed by immunoblotting technique after SDS-polyacrylamid gel electrophoresis. ATP-dependent protease was detected using antiserum raised against purified bovine adrenocortical ATP-dependent protease and avidin-biotin complex method. ATP-dependent protease activity was assayed using 14C-Caseins a substrate. Five cell fractions of homogenate placenta were electrophorated and antibody-stained using ATP-dependent protease, cytochrome P-450 scc and adrenodoxin antibody after the blotting, and the quantity was analyzed by the densitometry and the method of De Douve. We had the following results: 1) ATP dependent protease is present in human placenta and localized in mitochondria. 2) ATP dependent protease decomposes adrenodoxin reductase in human placenta. 3) ATP dependent protease present in almost same consistent in early and term placenta and there is no significant difference. It is suggested as follows that the ATP dependent protease in human placenta participates in the regulation of steroid hormone through the metabolism of steroid synthetic enzyme.

ATP-Dependent Proteases↗