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Biomedical subjects

S Wakisaka

Publications and source records attributed to S Wakisaka.

At least 217 records · Page 12Linked to original sources
PubMed1984

Substance P-like immunoreactivity in the pulp-dentine zone of human molar teeth demonstrated by indirect immunofluorescence.

Freshly-extracted human third molars were fixed in Zamboni fixative, demineralized with the mixture of EDTA and the fixative; substance P-like immunoreactivity (SPLI) was revealed by the indirect immunofluorescence technique of Coons. Substance P (SP) was observed in the pulp-dentine zone and the dental pulp. Some of SP-containing fibres ended at the odontoblast layer and did not reach the predentine; others terminated at the predentine surface or penetrated into the predentine. In the predentine, some of SP fibres accompanied odontoblast processes and ended near the mineralized dentine; others changed course transversely at various levels.

Dental Pulp↗
PubMed1984

Ultrastructure of granular materials in rat incisor enamel organ at early maturation stage.

With scanning electron microscopy, globules were seen in inter-cellular spaces between ameloblasts with ruffled borders and papillary cells at early maturation stage. Numerous globules were located on longitudinal folds between parallel ridges on the sides of ameloblasts, but only a few in the papillary layer. In previously-scanned specimens sectioned for transmission electron microscopy, the globules were composed of finely granular material. Vacuoles containing material identical to extra-cellular granules were observed in intra-cellular portions of the ameloblasts and papillary cells.

Ameloblasts↗
PubMed1984

[Clinical value of the sequential study of the uric acid in CSF in patients with cerebral diseases: Part I. Brain tumor and the effect of irradiation].

Uric acid is the end product of the purine metabolism in the human and is mainly excreted to the urine. The studies on cerebrospinal fluid (CSF) uric acid in patients with various neurological diseases were reported in the literature. In the present study the authors discussed the clinical value of the sequential study of the CSF uric acid content in patients with brain tumors. CSF was investigated for uric acid in 23 controls and 30 cases of brain tumor. The results were as follows: The mean value and standard deviation of the uric acid in CSF in controls was 0.23 +/- 0.13 mg/dl. The uric acid in CSF increased in patients with malignant brain tumor (0.49 +/- 0.22 mg/dl, p less than 0.005), but was in normal range in patients with benign brain tumor (0.32 +/- 0.13 mg/dl, 0.10 less than p less than 0.25). There was no significant correlation between CSF uric acid and CSF protein contents. Uric acid in the lumbar CSF was approximately 4 times higher than in the ventricular CSF in patients with brain tumor. The CSF uric acid had progressively increased during irradiation to the whole brain. The factors contributing to increase of the uric acid in CSF were thought to be increased permeability of blood-CSF barrier, global damage of brain tissue, increased nucleic acid catabolism in the central nervous system (CNS) for example in tumor, inflammation or immunoreaction, increased of xanthine, hypoxanthine or xanthine oxidase activity in the CNS, directly increased of plasma components into the CSF due to such as subarachnoid hemorrhage, intraventricular hemorrhage, bleeding in the tumor or surgical operation, dysfunction of the CSF dynamics.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenoma↗
PubMed1983

Scanning electron microscopy of the papillary layer of the rat-incisor enamel organ.

The enamel organ isolated from the maturation zone was treated with hydrochloric acid to remove such extracellular materials as collagen fibrils and basal lamina covering the papillary layer. Two types of the ridges were classified on the basis of their morphology and distribution: (1) globular and irregularly arranged ridges at the periphery of the early maturation zone, (2) elliptical and parallel ridges in the central part of this zone and over the whole of the late maturation zone. The fine surface structures of the ridges were characterized by numerous interdigitating cell processes.

Animals↗
PubMed1983

Possible explanation of radioresistance of glioblastoma in situ.

The response of human glioblastoma to radiation was studied in the logarithmic phase and in the plateau phase of growth, and was compared with those of HeLa S3 irradiated under identical conditions. There was no major difference in the in vitro survival curve parameters between glioblastoma and HeLa in the logarithmic growth phase. However, the surviving fractions for glioblastoma with radiation doses in the range used clinically were higher not only when irradiated in the logarithmic growth phase, but also when subcultured 6 hours after irradiation in the plateau phase, than those for HeLa treated under identical conditions. This suggests that the relatively low cure rate of glioblastoma can be partially explained by the intrinsic radiosensitivity in the logarithmic growth phase and by a high surviving fraction for cells irradiated in the plateau phase.

Cell Survival↗
PubMed1983

Sinus Pericranii.

Two cases of sinus pericranii are reported. X-ray films, taken with the direct injection of contrast medium into the extracranial blood cavity, made visualization of the intracranial venous systems possible in both cases. The pericranial blood sinuses were totally removed and communications through the osseous holes were closed with bone wax. The pericranial blood sinuses, surrounded by connective tissues, were located mainly in the subgaleal and supraperiosteal space. The literature is reviewed.

Adult↗
PubMed1982

Production in Escherichia coli of biologically active secretin, a gastrointestinal hormone.

A synthetic gene for porcine secretin was ligated with Pst I-cleaved pBR322 by its flanking synthetic Pst I linkers to produce a fused protein consisting of an amino-terminal portion of beta-lactamase and an entire secretin molecule. The hybrid plasmid was transferred into competent Escherichia coli cells. The plasmids, which were proved in our investigation to contain the secretin gene in the desired orientation, were then screened for the production of secretin. This was shown by radioimmunoassay and gel electrophoretic analysis of the polypeptides that were synthesized in E. coli minicells transformed with the hybrid plasmids. Secretin so produced, whose carboxy terminal residue may not be amidated in contrast with natural porcine secretin, showed the same activity in stimulating pancreatic secretion in a bioassay with anesthetized rats as does natural secretin.

DNA Restriction Enzymes↗
PubMed1982

[Computation of a radiodosage equivalent of the effect of bleomycin].

The responses of in vitro cultured mammalian cells to X-rays and bleomycin were compared for establishing a general rule to convert X-ray dose to bleomycin dose for a given biological effect. There was no significant difference in the radiosensitivities according to cell lines used in this experiment, while the responses of cells to bleomycin varied from cell to cell. The X-ray dose-response curves for the cells were convex upward if plotted on a semilogarithmical scale, while bleomycin dose-response curves were convex downward. These results indicated that the ratio of X-ray dose and bleomycin dose required for a comparable biological effect would vary with the X-ray dose and from cell to cell. Preliminary treatment with bleomycin sensitized mammalian L5 cells to X-rays. When the cells were exposed to bleomycin following irradiation, no significant synergestic effect was observed, in spite of the fact that the bleomycin dose was sufficient to result in a survival of 4.5%.

Adolescent↗
PubMed1981

Rathke's cleft cyst.

Two cases of Rathke's cleft cyst which produced symptoms of compression of the optic chiasm are described. The first case has been followed for five years since the operation. No evidence of recurrence has been noticed. The prognosis after a partial removal of the cyst wall seems to be good with this lesion. In the second case, there was clinical and laboratory evidence of hypopituitarism and the CT scan revealed suggestive findings to differentiate the cyst from a pituitary adenoma.

Craniopharyngioma↗
PubMed1980

Local cerebral glucose utilization in newborn and pubescent monkeys during focal motor seizures.

The [14C]deoxyglucose method was used to determine the rate of local cerebral glucose utilization (LCGU) in newborn and pubescent monkeys during focal motor seizures induced by injecting penicillin into the face-hand area of the right motor cortex. Seizures were studied in 3 newborn and 6 pubescent monkeys, and 3 newborn and 4 pubescent monkeys were used as controls. In controls, the pattern of glucose utilization within structures of the sensorimotor system was quite differenet at the two age levels; newborns showed far less activity, especially in the neocortex and striatum. In the monkeys with seizures, the unilateral increase in LCGU relative to the controls was greater in newborn than in pubescent monkeys except in the cerebral and cerebellar cortices. The increased glucose utilization in cortical and subcortical structures of the newborns was ipsilateral to the discharging lesin and lacked the well-defined pattern seen in the pubescent monkeys. In general, newborn brain was capable of supporting a focal motor seizure but lacked the precise clinical and electrographic expressions or efficient energy metabolism that accompany maturation of the brain at puberty.

Animals↗
PubMed1980

Propagation of focal motor seizures in the pubescent monkey.

The rate of local cerebral glucose utilization was determined for quantification of energy metabolism in macrostructures of the sensorimotor system during propagation of focal motor seizures in 24-month-old monkeys. The rate was measured in 4 control animals and in 4 monkeys each with seizures limited to the contralateral face, seizures of the contralateral face and upper extremity, and bilateral expression of seizures. Glucose utilization increased significantly, primarily unilaterally, with propagation. The increase was greatest in the sensory and motor cerebral cortices, putamen, and globus pallidus, was somewhat less in sensory and motor thalamic relay nuclei, and was least in the cerebellar cortex.

Animals↗