ICR-JCL strain mice were injected subcutaneously with 30 mg/kg body weight of cytosine arabinoside at 2, 3, and 4 days of age. This chemical prevented the production of the basket cells, stellate cells, and granule cells in the external granular layer of the cerebellum. Decrease in number of these microneutrons affected the noraml synaptic connections between the Purkinje cells and the microneurons, thus causing the disarrangement and abnormal arborization of the Purkinje cells. Of the three types of microneurons, the basket and a few stellate cells played a more important role in the disarrangement of the Purkinje cells and abnormal arborization of their primary dendrites than the granule cells did. Abnormal outgrowing directions of other smooth dendrites of the Purkinje cells were caused mainly by the diminution of stellate cells. Although parallel fibers were grossly decreased in number in the treated cerebellums, spines of the spiny dendrites of the Purkinje cells sprouted considerably in the 15-day-old mice, and then their morphological features remained even after 100 days of age.

Animals↗

PubMed1977

Cytosine arabinoside-induce transplacental dysgenetic hydrocephalus in mice.

Pregnant mice were injected with 30 mg of cytosine arabino side/kg body weight on 13-1/3 and 14 days of gestation. Within six hours after the first injection, pyknotic nuclei and nuclear debris were found at the matrix layer surrounding the lateral ventricles of the embryonic brain. Autoradiographic studies using tritiated thymidine revealed that most of the matrix cells exposed to cytosine arabinoside during DNA synthesis were killed by this dose of the drug. Twenty-four hours after the second injection, most of the matrix cells had disappeared. When experimental animals were examined after birth, they showed marked dilation of the lateral ventricles. The dilatation of the lateral ventricles was more pronounced in the parieto-occipital region. The young showed rapid enlargement of the cranial vault and subsequently died by 35 days of age.

Abnormalities, Drug-Induced↗

PubMed1975

Cytarabine and its effect on cerebellum of suckling mouse.

Suckling mice were injected with 30 mg/kg or 50 mg/kg body weight of cytarabine subcutaneously two, three, and four days after birth. Twenty-four hours after the final injection, these mice showed extensive necrosis of undifferentiated cells in the external granular layer of cerebellum. One or two weeks later, a new external granular layer regenerated but it did so only incompletely and regionally. The mice allowed to live longer showed impaired cerebellar function, cerebellar hypoplasia, and other malformations, including heterotopic granule cells in the molecular layer, irregularly arranged Purkinje cells, and scanty cellularity in the internal granular layer.

Aging↗

PubMed1975

Developmental abnormality of the retina caused by postnatal administration of cytosine arabinoside.

Suckling mice were injectd with 30 or 50 mg cytosine arabinoside/kg body weight 2, 3 and 4 days after birth. Within 6 h after the first injection, a number of pyknotic nuclei were found at the inner portion of the undifferentiated nuclear layer of the retinas. 24 h after the final injection, the retinas of these mice showed the large cavity resulting from necrosis of undifferentiated cells and the rosettes in the nuclear layer. The retinas of 20-day-old treated mice showed conspicuous abnormalities with many rosettes in the outer nuclear layer, heterotopic cell mass in the inner plexiform layer, and irregular arrangement of the ganglion cells. 240 days later, histological abnormalities in the retinas of the treated mice were considerably improved except for rosettes, which were still numerous but changed into arch-like structures.

Animals↗

PubMed1973

Cytosine arabinoside and rosette formation in mouse retina.

Explore the source record for details and available documents.

Animals↗