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Biomedical subjects

S Verghese

Publications and source records attributed to S Verghese.

29 records · Page 2Linked to original sources
PubMed1987

Epithelioid cell granuloma induction in the guinea pig by haptenated Mycobacterium leprae.

Fluorescein isothiocyanate (FITC)-conjugated Mycobacterium leprae (FITC-M. leprae) was injected intradermally into the ears of guinea pigs and granuloma formation in the draining postauricular lymph nodes was studied. At 2 weeks, there was an increase in weights and infiltration of the draining lymph nodes in half of the animals injected with FITC-M. leprae. At 5 weeks, there was a significant increase in the weights and infiltration of these draining lymph nodes in the guinea pigs injected with haptenated M. leprae compared with those injected with unconjugated M. leprae. At 5 weeks, there was also a significant increase in delayed type hypersensitivity responses to 25 micrograms purified protein derivative. Histologically, epithelioid cell granulomas were seen in these lymph nodes as early as 2 weeks when FITC-M. leprae was used as the source of antigen. Enhancement in the immunogenicity of M. leprae by conjugation with FITC has been postulated.

Animals↗
PubMedDate not supplied

Cryopreservation of cardiac homografts.

A homograft valve bank for cryopreservation of cardiac homografts was established at the Institute of Cardiovascular Diseases in July 1995. From July 1995 to February 1999, 169 donor hearts were processed. All except four hearts were procured post mortem. Aortic valves (149) and pulmonary valves (139) were the common homografts dissected out for use. The valves were immersed in a cocktail of five broad spectrum antibiotics and antifungals for an average of 48 to 72 hours before cryopreservation. Fifty-three (35.57%) aortic and 42 (30.21%) pulmonary valves had to be discarded for various reasons like fungal contamination, failure to sterilise, HBsAg positivity etc.; 153 homografts have been released for use so far. Analysing the usual methods of procurement, sterilisation protocol, culture and cryopreservation used for cardiac homografts at this centre, this paper recommends observance of timeliness, use of appropriate media for preservation of heart parts, administration of specific drugs and safeguards necessary for cryopreservation procedure.

Cryopreservation↗
PubMedDate not supplied

Primary aspergillosis of the breast. Report of a case with fine needle aspiration cytology diagnosis.

We report an unusual case of primary Aspergillus flavus fungal colonization in a subareolar breast mass in a middle-aged woman. The patient was clinically normal, with no evidence of immunosuppression. Fine needle aspiration cytology was carried out as a routine procedure to rule out malignancy, and Aspergillus infection was diagnosed on cytology. Cultures from fine needle aspiration cytology and the surgical biopsy material yielded a heavy growth of A flavus. The etiology and pathology of this fungal lesion presenting as a breast mass are discussed.

Aspergillosis↗
PubMedDate not supplied

Fungal endocarditis following cardiac surgery.

This study analysed cases of fungal endocarditis following cardiac surgery encountered in our institute between January 1994 and December 1997. A total of 887 prosthetic valve and 114 homograft valve replacements were performed during the study period. In this group, eight cases of fungal endocarditis (aged 14-53 years) were encountered during the follow-up of which five were caused by candida species and three by aspergillus species. Four cases involved aortic homografts, two mitral prosthesis, one an aortic prosthesis and one was a Goretex patch endocarditis. Seven patients had early endocarditis; all presented with fever and two had embolic manifestations. Seven patients each had positive blood cultures and echocardiographically demonstrable vegetations. Despite management with amphotericin B and early repeat surgical interventions, six had a fatal outcome. One patient is doing well at nine months follow-up after a second valve replacement and one is lost to follow-up.

Adolescent↗