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Biomedical subjects

S V Okovityĭ

Publications and source records attributed to S V Okovityĭ.

10 recordsLinked to original sources
PubMed2004

[The influence of hepatoprotector 2-ethylthiobenzimidazole hydrobromide (bemithyl) on the content of glycogen in cirrhotic rat liver hepatocytes located in various microenvironments].

Using absorption and fluorescent cytophotometry methods, glycogen contents were studied in hepatocytes located in liver lobules and in hepatocytes, which make the general population of these cells in normal and cirrhotic rat liver. In cirrhosis, the content of glycogen in hepatocytes located in lobules obviously rises in comparison with the norm, but to a lesser degree, than in hepatocytes making the general population of these cells in cirrhotic liver. The content of glycogen in hepatocytes, located in lobules of pathologically changed liver in bemithyl treated rats, did not differ from the norm. At the same time, the glycogen content in hepatocytes, representing the general population of these cells in cirrhotically altered bemithyl injected rat liver, remained higher than in the norm. The data obtained indicate that distinctions in particular cell microinvironment, obviously present in cirrhotic liver, render essential influence on hepatocyte functional activity.

Animals↗
PubMed2003

[Dynamics of glycogen content in the normal and cirrhotic liver following glucose administration to starving rats].

Using biochemical, cytofluorimetric and television cytophotometric methods, glycogen contents were studied in normal and cirrhotic rat liver at various intervals after glucose administration to fasting animals. The obtained data indicate that after a 48 h fasting glycogen contents in normal and cirrhotic liver are equally poor. A marked rise of glycogen content in cirrhotic liver was observed only 20-30 min after glucose administration to rats. It has been established that at all intervals after glucose administration to rats hepatocytes of the portal lobule zone, both in normal and in cirrhotic liver, accumulate more glycogen than those of the central zone. Again, the intensity of glycogen accumulation in cirrhotically altered liver is significantly lower than in normal liver, due, presumably, to a lower rate of glycogen synthesis in pathologically changed liver.

Animals↗
PubMed2002

[Effect of bemythyl on carbohydrate metabolism in cirrhotic rat liver].

Effect of actoprotector bemitil (2-ethylthiobenzimidazole hydrobromide) on glycogen content and activities of glycogen synthase, glycogen phosphorylase, and glucose-6-phosphatase was studied in cirrhotically altered rat liver. The contents of glycogen and its fraction were determined a cytofluorimetrically (Kudryavtseva et al., 1974). In cirrhosis, the total glycogen content in hepatocytes increases by nearly 3 times, while the amount of a stable fraction of glycogen rises by 7.5 times. Glucose-6-phosphatase activity fell to the level of 25% compare to the norm. Activities of glycogen synthase and glycogen phosphorylase in the cirrhotic liver did not differ from the norm. In cirrhotically altered liver, bemitil produced a decrease in the total glycogen content due to a decrease in glycogen synthase activity in an increase in glucose-6-phosphatase and glycogen phosphorylase activities. The above results suggest a favorable effect of bemitil on cirrhotic liver.

Animals↗
PubMed2002

[Features of the effect of bemethyl on glycogen metabolism in hepatocytes of pathological changed human liver].

Effect of actoprotector bemithyl (2-ethylthiobenzimidazole hydrobromide) on glycogen metabolism in hepatocytes of patients with chronic hepatitis and liver cirrhosis was investigated. Using cytofluorimetric method, the content of glycogen and its fractions in isolated hepatocytes was measured. The treatment with bemithyl resulted in a decrease in glycogen levels in hepatocytes, and in a marked restoration of fractional glycogen composition as compared to the basic therapy. Besides, it was established that the degree of glycogen decrease in cells of patients with chronic hepatitis depended on the increase of glucose-6-phosphatase activity (r = 0.75, P < 0.05), and on the levels of glycogen in hepatocytes prior to bemitil treatment (r = = 0.87, P < 0.01). Positive changes in glycogen metabolism after bemithyl treatment are pronounced in patients with chronic hepatitis. These positive alterations take place simultaneously with the conservation of basic structural disturbances in the liver parenchyma. However, even in this case, the indices of glycogen metabolism do not reach the normal levels.

Antioxidants↗
PubMed2000

[Metabolic heterogeneity of glycogen in hepatocytes of patients with liver cirrhosis].

Concentrations of the total glycogen (TG) and of its labile and stable fractions (LF and SF, respectively) were determined in hepatocytes of portal and central zones of the normal human liver and in the liver of patients with cirrhosis of viral and alcohol etiology. Using the PAS reaction, TG and its LF and SF were revealed in histological sections of the material obtained by liver punction biopsies. Concentrations of TG and its fractions were measured by television cytophotometry. In liver cirrhosis, concentrations of TG, LF, and SF in both zones of the hepatic lobule were much higher than in the normal liver. The ratio between hepatocyte TG concentration in the portal zone and that in the central zone (P/C ratio), both in norm and in viral cirrhosis, exceeds 1.0 to reach, respectively, 1.26 +/- 0.02 and 1.03 +/- 0.01. The glycogen fraction composition in cells of both liver lobule zones in viral cirrhosis does not significantly differ from that in norm. On the contrary, in the liver of patients with alcoholic cirrhosis, the P/C ratio falls to 0.82 +/- 0.02 to be accompanied by qualitative changes in glycogen composition.

Humans↗
PubMedDate not supplied

[Effects of bemethyl, ethomersol, and yakton on the liver regeneration after partial hepatectomy].

It is experimentally demonstrated for the first time that the new drugs bemithyl, etomerzol, and yakton are capable of accelerating the process of liver regeneration following partial hepatectomy. The drugs produce a hasty gain in the mass of liver, increase in the content of nucleic acids and glycogen, and improve the functional state, as manifested by a decrease in the blood bilirubin and a reduction in the hexenal sleep duration. Bemithyl, etomerzol, and yakton produce a positive effect upon the liver morphology and the intracellular regeneration process. The repair activity of the new drugs exceeds that of a combination of the well-known regeneration stimulants riboxin and potassium orotate, representing derivatives of purine and pyrimidine bases.

Animals↗
PubMedDate not supplied

[Immune mechanisms of hepatoprotector effects of etomersol and thymogen].

The reparation-regeneration effects of the actoprotector etomerzol and the immunomodulant thymogen can be mediated by certain units of the immune system, in particular, by thymus. Both thymogen and etomerzol loss the hepatoprotector activity on the background of thymectomy, but still produce an immunomodulant effect by stimulating the production of antibody-forming cells in the spleen in response to changes in the immune system induced by various stressor factors (thymectomy, hepatectomy, or their combination). This property of etomerzol, together with the absence of influence upon the immune state of intact experimental animals, is evidence of a dominating immunomodulant activity of this drug.

Adjuvants, Immunologic↗
PubMedDate not supplied

[New derivatives of triazino- and imidazoindole with hepatoprotective activity].

The effect of 30 indole derivatives (mainly condensed) containing a thio-carbamide fragment was investigated in rats using a model of carbontetrachloride-induced hepatitis and a model of partial hepatectomy (for some compounds). Among indoles, 1,2,4-triazino[5,6-b]- and [6,5-b]indole as well as imidazo[4,5-b]indole series the compounds, decreasing concentration of alanine aminotransferase, aspartate aminotransferase and total bilirubin in blood serum and reducing hexenal sleep duration were found. The compounds exceeding in this respect riboxine, potassium orotate and essentiale (used for comparison) and posessing sufficient therapeutic index were found. The correlation between biological activity and ionisation constants was observed for triazinoindole derivatives, while for some indices of liver state the correlation with ionisation constants and distribution coefficients in octanol/water system was found as well. High activity was most probable at pKa < or = 8. For aminoallcyl thioderivatives of triazinoindole the protective effect increased on substituting the aminogroup for hydrogen atom at 8-position, on increasing the side chain length from 2 to 3 methylene links and on replacing of acyclic aliphatic amines residues in a side chain by cyclic amines--pyrrolidine, N-methylpiperazine and morpholine rather than piperidine which increased toxicity. There was no correlation between hepatoprotective and antihypoxic activity while for actoprotectors the probability of liver protection was increased. The results indicate perspectives in searching new hepatoprotective agents among the indicated series of indole derivatives.

Animals↗