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Biomedical subjects

S V Filippov

Publications and source records attributed to S V Filippov.

At least 19 recordsLinked to original sources
PubMed2006

[Effect of amino acids on cell proliferation and apoptosis in organotypic tissue culture of young and old rats].

There was studied in brain cortex, spleen and liver organotypic tissue culture the effect of effective concentrations (0.05 ng/ml) of 20 amino acids on the development of proliferative activity and apoptosis in peripheral fields of explants of rats of various age: 1 month and 18 months old. In the young tissues, the most frequent activity (3 times) was found while using the histidine, asparagine acide, valine, threonine, proline, leucine, tyrosine, methionine, and the less frequent (1 time)--while using lysine, glutamine, cysteine. Almost half number of the amino acids (11) was active in the old tissue cultures, as compared to the young tissues (19). The summary effectiveness of the amino acids was greater in young tissues (42), as compared to the old tissues (11). The data obtained suggest, that the bioregulator peptides should be necessary used to correct the less sensitivity of the tissues to the amino acids in old age.

Aging↗
PubMed2006

[The tissue-specific effect of synthetic peptides-biologic regulators in organotypic tissues culture in young and old rats].

The synthetic peptides--cardiogen, bronchogen, prostamax and pancragen were used to investigate their effect on organotypic tissue culture of explants from heart, lungs, prostatic gland and pancreas of young (3-week old) and aged (18-month old) rats. The peptide effective concentration of 0.05 ng/ml showed a stimulating effect in appropriate tissue cultures as compared to the control explants in all groups of rats. So these bioregulatory peptides can be used in clinic practice for the stimulation of reparative processes in the appropriate tissues while ageing.

Aging↗
PubMed2002

[Functional morphology of an organotypic liver culture exposed to the peptide livagen].

In this study the results of the tetrapeptide livagen effect on functional morphological characteristics have been produced. Under the data of the immunocytochemical and morphometrical analysis, tetrapeptide stimulates structural and functional homeostasis of cell populations in the liver culture. It was demonstrated that the main activity of investigated peptide had been directed to the stabilisation of morphological safety and reinforcement of the processes of cellular and intracellular forms of regeneration.

Animals↗
PubMed1998

[The comparative clinico-immunological characteristics of the course of mandibular fractures in patients in Yakutsk and Moscow].

Ninety-seven patients with mandibular fractures were examined: 55 in Yakutsk and 42 in Moscow. In the Yakutsk patients the inflammatory process much more frequently progressed to the development of infectious inflammatory complications at the site of fracture than in Moscow. More expressed shifts in the immunogram were observed mainly in the patients who later developed infectious inflammatory complications.

Adolescent↗
PubMed1997

[A comparative analysis of the course of odontogenic phlegmons of the face and neck area in patients from Yakutsk and Moscow].

A total of 126 patients with odontogenic inflammatory diseases were examined, 54 of these in Moscow and 72 in Yakutsk. The clinical and immunological parameters were assessed on days 2 and 6 after similar operations. In residents of Yakutsk the inflammatory process was associated with a more expressed intoxication and coursed 1.5 to 2 days longer than in Muscovites; moreover, the residents of Yakutsk developed higher leukocytosis and a drop of T-lymphocyte level with the predominance of T-helpers. Hence, odontogenic inflammations take mainly a hyperergic course in Yakutsk and a normergic one in Moscow.

Adolescent↗
PubMed1995

Heterogeneity of smooth muscle cells in embryonic human aorta.

Cellular composition of aortas from 5- to 12-week and 18- to 28-week-old human embryos were investigated using immunocytochemistry, scanning and transmission electron microscopy. The aorta of the 5- to 12-week-old embryos consisted of three sublayers differing in cellular composition. The inner sublayer adjacent to the endothelium contained round and ovoid cells with synthetic phenotype. In the intermediate sublayer, spindle-like cells ultrastructurally similar to smooth muscle cells were found. Cells of the outer sublayer resembled fibroblasts or poorly differentiated mesenchymal cells. There were not definite morphological borders between sublayers. In the 18- to 28-week-old embryo aorta the intima was separated from media by internal elastic lamina. Intimal and innermost medial cells had predominately stellate shape and synthetic phenotype. The outer part of media contained spindle-like cells that had well developed contractile structures. Both the 5- to 12-week-old and the 18- to 28-week-old embryo aortic cells were positively stained for alpha-actin and myosin and negatively stained for macrophage antigens. Thus, the majority of embryo aortic cells appeared smooth muscle cells, however there was a regional difference in shape and synthetic state of these cells.

Aorta, Thoracic↗
PubMed1993

[Changes in the spatial organization and structure of the smooth-muscle cells of the tunica media of the major arteries during lengthwise stretching in situ].

It has been shown that in vivo stretching of the arterial wall leads to the loss of cell-cell contacts between medial smooth muscle cells (SMC), and to the change in the cell cooperation type. It is accompanied by the increase in SMC proliferative activity, and by changes in their shape and phenotype, from the contractile to the synthetic one. The increase in the number of extracellular connective tissue structures occurs in the intercellular space.

Animals↗
PubMed1993

[Cellular orientation and form in the elastic-hyperplastic layer of the human aortic intima in the area of the ostia of the intercostal arteries].

Using the Hatcher monolayer method, it has been shown that the aortic intimal smooth muscle cells, disposed on the flow divider of an intercostal artery, have predominantly spindle-like shapes to be oriented perpendicularly to the long axis of the vessel. Unlike, in the proximal and lateral sites of the intercostal ostium the intima is represented by stellate cells lacking any orientation. These distinctions may be presumably determined by the irregular distribution of hemodynamic loading on the aortic intima in different regions around the ostium of an intercostal artery.

Adult↗
PubMed1993

[Changes in the orientation of the smooth-muscle cells of the tunica media from major arteries under constant lengthwise stretching in situ].

It has been shown that changes in the orientation of arterial smooth muscle cells during a constant longitudinal stretching of the artery in vivo are not similar in different sections of the stretching zone. Cells in the proximal and distal sections keep their orientation but this orientation differs from that of smooth muscle cells in the control arteries. Cells in the central part of the stretching region lose their definite orientation to settle randomly.

Animals↗