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Biomedical subjects

S Ustaçelebi

Publications and source records attributed to S Ustaçelebi.

At least 19 recordsLinked to original sources
PubMed1998

Anti-Kp 90 IgA antibodies in the diagnosis of active tuberculosis.

BACKGROUND: Accurate diagnosis of active tuberculosis (TB) has been difficult historically, yet a great demand persists for a rapid and reliable diagnostic method. Detection of Mycobacterium tuberculosis anti-Kp 90 IgA antibodies is one of the more novel techniques. STUDY OBJECTIVES: To evaluate the diagnostic value of a recently developed enzyme-linked immunosorbent assay (ELISA) test, which detects IgA antibodies against M tuberculosis Kp 90 antigen, and to compare the results with conventional diagnosis and the polymerase chain reaction (PCR) method. PARTICIPANTS: Serum, ethylenediaminetetraacetic acid (EDTA)-blood, and body fluid samples were obtained from 51 patients with active TB and 71 control subjects. The clinical diagnosis of TB was supported by a positive culture (n = 6), detection of acid-fast bacilli on smear (n = 35), or both (n = 10). MEASUREMENTS AND RESULTS: IgA antibodies were detected in sera and/or body fluid samples from 82% of patients with TB and 10% of controls. M tuberculosis DNA was detected in body fluid sample of 96% and blood sample of 49% of patients with TB by PCR. None of the blood and 5.6% of the body fluid specimens from controls were PCR-positive. CONCLUSIONS: Anti-Kp 90 IgA antibodies were detected using ELISA in 78% of serum and 69% of body fluids from patients with TB, therefore, this test is promising for the diagnosis of active TB and appears to be more reliable, particularly for body fluid samples.

Adolescent↗
PubMed1992

[Detection of BK virus activation in renal and bone marrow transplantation patients using serological and virological methods].

In this study, 10 renal and bone marrow transplant recipients' sera samples taken before and after transplantation and urine samples taken after transplantation were investigated for evidence of reactivation of BK virus, a human polyoma virus. BK virus reactivation was detected in 4 transplant patients serologically by hemagglutination inhibition, ELISA-IgG and ELISA-IgM tests. However we did not succeed to isolate BK virus in Vero cell lines from any urine samples. Only one patient's urine sediment whom serologic reactivation was detected, contained uroepithelial cells with intranuclear inclusion bodies characteristic for BK virus when examined by Giemsa staining.

Adolescent↗
PubMed1992

[New vaccines].

In this article, schedule for active immunization of normal infants and children in the routine strategy have been reviewed and some of the new vaccines were defined.

AIDS Vaccines↗
PubMed1991

[New human polyomavirus; BK virus antibody levels in different age groups using the hemagglutination inhibition test].

In this study, 1123 human sera samples obtained from healthy persons belonging to various age groups were tested in order to detect the distribution of antibody levels against the new human polyomavirus, BK. All sera samples were tested by hemagglutination inhibition (HI) test and 881 (78.5%) of those were found to be positive and 242 (21.5%) were negative. The percentage of positivity was 65.3% between the ages of 1 to 5 years, and over 80% in the group aged 6 years or older. Furthermore, between 1 to 10 years of ages HI antibody titers were higher than the older age groups. Our results indicate that antibody to BK virus infections are remarkably frequent in our population and acquisition of BK virus appeared to be common in childhood.

Adolescent↗
PubMed1991

[New human polyomavirus: comparison of hemagglutination inhibition, ELISA and complement fixation tests for BK virus serology].

In this study, 110 sera samples taken from healthy persons were tested for BK virus antibodies by using hemagglutination inhibition (HI), ELISA and complement fixation (CF) tests to detect the sensitivity, specificity and agreement of these tests. The results of each individual test was compared with others for sensitivity and specificity. Specificity found to be 96% when HI and CF tests were compared. Sensitivity also found to be 92.7% when CF and ELISA tests were compared. Agreement of HI and ELISA tests were 88%.

Antibodies, Viral↗
PubMed1990

[The in vitro activity of ciprofloxacin against clinically-isolated strains of Pseudomonas aeruginosa and comparison with some other antibiotics].

In this study the susceptibility to ciprofloxacin of 100 Pseudomonas aeruginosa strains isolated from various clinical specimens was investigated by disk diffusion test and macrodilution method, and was compared with other various antibiotics. Both of the methods showed that 98 of the strains were susceptible to ciprofloxacin. MIC50 of ciprofloxacin was found to be 0.12, and MIC90 0.50 mcgr/ml for these strains. Pseudomonas aeruginosa strains were found to be highly susceptible to ciprofloxacin in in-vitro conditions.

Amikacin↗
PubMed1990

[Herpes simplex type I virus observed in the superior cervical ganglion from human cadavers and autopsy materials].

Herpes Simplex Type I (HSV I) causes some infections such as herpes labialis, encephalitis, keratoconjunctivitis and also some cranial nerve syndromes such as acute vestibular neuritis, migraine and Meniere's disease in human. We used 4 fixated and 16 fresh cadavers to isolate HSV I virus from the Superior Cervical Ganglia. The ganglia materials are inoculated to PRK (primary rabbit kidney), VERO (African Green Monkey Kidney) and BHK 21 (Baby Hamster Kidney) cell lines in order to isolate the virus. We isolated HSV I virus from 12 fresh cadavers' cervical ganglia (75%) and neutralisation test is performed in order to characterize HSV I. But we could not isolate the virus from any of the fixated cadavers.

Adult↗
PubMed1989

[AIDS: acquired immunodeficiency syndrome].

In this article the molecular biology of HIV (Human Immunodeficiency Virus) which is the causative agent of acquired immune deficiency syndrome and the pathogenesis, epidemiology and clinical picture of the syndrome, the current therapeutic approaches and the developments in the vaccination studies were reviewed.

Acquired Immunodeficiency Syndrome↗
PubMed1989

[The viral etiology of amyotrophic lateral sclerosis].

Roles of immunologic factors and slow virus infections in the aetiopathogenesis of amyotrophic lateral sclerosis (ALS) have recently gained significance. For this purpose, presence of poliovirus type I, measles and herpes simplex (HSV) type I antibodies were determined in the sera of ALS patients by complement fixation technique. Significant increase in antibody titers for poliovirus type I and HSV type I were determined in sera of ALS patients in comparison with controls and patients with other neurologic disorders.

Adult↗
PubMed1988

[Assessment of viral factors in the etiopathogenesis of Sjögren's syndrome].

This study has been carried out in serums of 32 Sjögren's syndrome (SS) patients -16 primary and 16 secondary- who have been followed-up for 3 years in Hacettepe University Medicine Faculty Ophthalmology Department. The serum levels of cytomegalovirus (CMV) antibodies and the antibody titers of herpes simplex virus (HSV) type I has been determined by the ELISA test and the complement fixation method respectively. The viral factors that play a role in the pathogenesis of SS have been discussed in regard to the results of the study.

Antibodies, Viral↗
PubMed1988

[New human papovaviruses: JC and BK].

BK and JC viruses are human papovaviruses widespread in human population. Primary infection with those viruses are generally asymptomatic and reactivation occur upon immunodepression. JC virus found to be associated with Progressive Multifocal Leukoencephalopathy. In the other hand BK virus mostly reactivated after immunosuppression. Both viruses have oncogenic potential when inoculated subcutaneously or intracerebrally to experimental animals. However, there is no direct relation has been shown yet for association of those new human papovaviruses with human malignancies.

BK Virus↗
PubMed1988

[The immunologic processes of the etiopathogenesis of Sjögren's syndrome].

In this study 32 Sjögren's syndrome cases--16 primary and 16 secondary--have been followed-up for 3 years in Hacettepe University Medicine Faculty Ophthalmology Department. The serum levels of rheumatoid factor and complement have been determined and autoantibodies have been looked for by the indirect immunofluorescence technique. The immunological processes that play a role in the pathogenesis of Sjögren's syndrome have been discussed with regard to the results of the study.

Antibodies, Antinuclear↗
PubMed1988

[Detection of seropositivity against rubella virus in women in the reproductive age and comparison of the rubella hemagglutination inhibition test and the ELISA-IgG test].

In this study Hemagglutination Inhibition (HI) and ELISA-IgG tests used for Rubella serology were compared in terms of specificity and sensitivity In addition, 100 sera samples taken from women in pregnancy age were tested for Rubella antibodies. The results indicated that the sensitivity of Rubella ELISA IgG test is 100% and the specificity is 97.4%. The seropositivity of women in pregnancy age was found to be 93% by Rubella ELISA-IgG test.

Adolescent↗