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Biomedical subjects

S Ueda

Publications and source records attributed to S Ueda.

At least 1,315 records · Page 73Linked to original sources

A minimally invasive approach to rectal cancer--sacrolaparoscopic approach.

BACKGROUND/AIMS: To investigate the feasibility of a technique with minimal skin incision, while retaining a rate of cure and safety equivalent to conventional rectal amputation, by making use of the advantages of laparoscopic procedures, we performed a minimally invasive laparoscopic rectal amputation. METHODOLOGY: Six patients suffering from rectal cancer with cardiac and/or respiratory disorders underwent laparoscope-assisted rectal amputation. The procedure was performed in three steps: 1) sacral approach, 2) laparoscope-assisted abdominal approach under CO2 insufflation, and 3) extracorporeal resection of the inferior mesenteric artery (IMA) and stoma making without CO2 insufflation. RESULTS: Intra-operative cardiopulmonary functions were maintained within normal range during CO2 insufflation. Although all patients had severe respiratory or cardiac disorder or diabetes mellitus, no complications were observed during and after surgery. The post-operative course was uneventful for our patients, each of whom could eat on the first post-operative day and walk on the third post-operative day. All patients were discharged from the hospital uneventfully. CONCLUSIONS: Laparoscope-assisted rectal amputation is technically feasible, adequate tumor excision can be achieved with it and post-operative recovery is improved. Sacrolaparoscopic rectal amputation appears to be a safe alternative procedure for patients with rectal cancer and even with severe cardiopulmonary disorders.

Aged↗

Effect of a synthetic analog of prostaglandin E1 on the intestinal mucosa of methotrexate-treated rats.

Administration of methotrexate to rats sometimes induces small intestinal damage. A synthetic analog of prostaglandin E1, OP-1206 [17S,20-dimethyl-trans-delta2-prostaglandin E1] may possibly provide therapeutic benefits to help recovery from such small intestinal damage. The purpose of this study was to evaluate the effect of OP-1206 on methotrexate-induced small intestinal damage in rats. Methotrexate (15 mg/kg body weight) was orally administered to rats once daily for 5 days. OP-1206 (0.5 microg/kg body weight) was orally administered to rats twice a day for 5 days and on the 6th day the small intestine of the rats were examined histologically and biochemically. The methotrexate treatment of rats caused a severe histological change in the small intestinal mucosa, whereas the treatment combined of OP-1206 with methotrexate showed similar histological features of the small intestinal mucosa as that of the control rats. On the other hand, an acute intestinal inflammation was evaluated by determining myeloperoxidase activity. The myeloperoxidase activity in the small intestinal mucosa of the methotrexate-treated rats increased remarkably, whereas that of the methotrexate and OP-1206-treated rats was significantly lower than that of the methotrexate-treated rats. Thus, it was shown histologically and biochemically that OP-1206 was effective in protecting the small intestine from methotrexate-induced damage.

Alprostadil↗

New diagnostic approach to intracystic lesions of the breast by fiberoptic ductoscopy.

Intracystic tumors of the breast are uncommon and, at the time of ultrasonography and aspiration cytology, it is difficult to distinguish cancer from a benign tumor. The Fiberoptic Ductoscopy System (FDS) is an emerging technique allowing direct visual access to the ductal system of the breast. FDS was inserted through the cannulae into the cavity and we observed the intracystic tumors (3 intracystic papillomas and 2 intracytsic papillary carcinomas). The appearance of the malignant tumors was irregular, rough-shaped and they tended to bleed. On the contrary, benign tumors had smooth surfaces without bleeding. Cytological findings showed malignant cells in one out of two breast cancer patients. In addition, in the immunohistochemical study of resected tumor tissues from 5 patients, we observed positive reactions with anti-ErbB-2 antibody in 2 intracystic papillary carcinomas. In contrast, none of the histologically confirmed benign lesions (3 intracystic papillomas) gave positive results. In conclusion, the use of FDS as a non-invasive technique may provide valuable information.

Adult↗

Differentiation of oncogenic and nononcogenic strains of Marek's disease virus type 1 by using polymerase chain reaction DNA amplification.

Differentiation of oncogenic and nononcogenic strains of Marek's disease virus type 1 (MDV1) was attempted by polymerase chain reaction (PCR) using the primers chosen from the sequence within the long inverted repeats of MDV1 DNA. PCR of the DNAs extracted from oncogenic-strain-infected cells and Marek's disease tumor cell lines produced a major product containing two or three copies of 132-base-pair (bp) repeat units, whereas PCRs of the DNAs extracted from nononcogenic-strain-infected cells yielded amplified products with various sizes corresponding to the number of 132-bp repeat units. The primers chosen from the glycoprotein A genes of MDV1 and herpesvirus of turkeys also were used for determination of their serotype specificity. The PCR procedure was found to be a simple and sensitive procedure for identification of MDV1 and herpesvirus of turkeys and for estimation of oncogenicity of MDV1.

Base Sequence↗

Cerebellar glioblastoma genetically defined as a secondary one.

We report here the case of a 29-year-old woman with cerebellar glioblastma. In the present case, tumor lesions were observed in each cerebellar hemisphere. The left-side lesion was diagnosed as glioblastoma, and the right-side lesion as malignant astrocytoma by histopathology. Immunohistochemistry revealed that the tumor cells of the left-side lesion was positive for p53, whereas epidermal growth factor receptors (EGFR) were negative in tumor cells from both sides. Genetic alterations were investigated using a genome DNA microarray (GenoSensor Array 300), which has led us to define this tumor as a secondary glioblastoma. The clinical presentation and genetic findings of this relatively rare entity are discussed.

Adult↗

Immunoperoxidase staining for the detection of herpes simplex virus antigen in cervicovaginal smears.

Destained cervicovaginal smears from eight patients with herpes simplex virus (HSV) infections were stained by means of the peroxidase-antiperoxidase (PAP) technique to demonstrate the presence of the HSV type 2 (HSV-2) antigen. Positive results were obtained in six of the eight cases, with intense staining for the HSV-2-specific antigen throughout the cytoplasm and nuclei of cells having a ground-glass nuclear appearance as well as in multinucleated giant cells. Virus isolation was successfully performed for the HSV-2-positive case that also had a histologically confirmed squamous-cell carcinoma of the cervix. The combined use of cytology and the PAP staining technique was of great value in the demonstration of cervical HSV infections.

Adult↗

An enzyme-linked immunosorbent assay for serodiagnosis of coccidiosis in chickens: use of a single serum dilution.

A simplified enzyme-linked immunosorbent assay (ELISA) was used to survey broiler chickens for coccidial infection. With an antigen derived from Eimeria tenella oocysts and a single serum dilution of 1:100, the absorbance values were directly used for the evaluation of antibody levels. When the detection level, determined with sera from specific-pathogen-free chickens, was used as the negative standard, randomly selected sera from broiler chickens essentially were negative. Sera were periodically collected from two flocks of commercial broilers reared under medication, and their ELISA titers were compared with their excretion of oocysts. When the oocyst isolation rate indicated that infection was severe, a substantial number of chickens showed high absorbance values in ELISA. These results indicate that coccidial infections in the field can be detected serologically with ELISA.

Animals↗

Partial protection against Marek's disease in chickens immunized with glycoproteins gB purified from turkey-herpesvirus-infected cells by affinity chromatography coupled with monoclonal antibodies.

Glycoproteins gB of Marek's disease virus (MDV) and herpesvirus of turkeys (HVT) related to virus neutralization were purified from HVT-infected cells by affinity chromatography. Immunization of chickens with purified glycoproteins gB resulted in partial protection against MD. Neutralizing antibodies were detected in chickens immunized with HVT-gB.

Animals↗

Intraarterial infusion of cisplatin with and without preoperative concurrent radiation for urinary bladder cancer: a preliminary report.

We evaluated the clinical efficacy of treating urinary bladder cancer by intraarterial infusion of cisplatin using an implanted reservoir with and without preoperative concurrent radiation. No previous reports have compared the results obtained by these two methods of treatment. Twenty-three patients with bladder cancer were treated by intraarterial infusion of cisplatin using an implanted reservoir with (n = 13) and without (n = 10) concurrent radiation. The cisplatin plus radiation group received intraarterial cisplatin at a total dose of 200-400 mg and concurrent radiation to a total dose to 30 Gy. The cisplatin group received intraarterial cisplatin at a total dose of 100-600 mg. In the cisplatin plus radiation group, the overall tumor response rate was 92%. Seven of 13 (53%) patients obtained complete response (CR), and the 2-year actuarial survival rate was 92%. Only one of the seven complete responders has had a local recurrence. In the cisplatin group, the overall tumor response rate was 90%. Four of 10 (40%) patients obtained CR, and median survival was 8 months. Three of the four complete responders have had local recurrence. There was no significant difference between these two groups in the frequency of side effects. Concurrent radiation therapy with intraarterial cisplatin resulted in a very low rate of recurrence of bladder cancer compared with intraarterial cisplatin therapy alone. This method was useful for urinary bladder cancer and may become the treatment of choice for this type of cancer.

Aged↗

Persistent common bile duct hematoma caused by a small cancer: case report.

We report a case in which an intrabiliary duct hematoma secondary to a coexisting smaller choledochal cancer was observed for more than a month. Initial noncontrast enhanced X-ray computed tomography (CT) showed the hematoma as a high-attenuation mass, leading to the preoperative diagnosis of choledochal stone or calcified tumor.

Aged↗

Protective effect of oral administration of killed Haemophilus paragallinarum serotype A on chickens.

Orally administered bacterin of killed Haemophilus paragallinarum effectively induced production of serum hemagglutination-inhibition antibodies in chickens, and all immunized chickens were protected from subsequent infection. Although one intramuscular dose of 10(8) cells adjuvanted with aluminium phosphate gel (equivalent to commercial vaccine) induced protective immunity, two oral doses of 10(10) cells each with no adjuvants were required to induce effective immunity.

Administration, Oral↗

Sequence comparisons of a highly virulent infectious bursal disease virus prevalent in Japan.

Variable cDNA regions in the VP2 gene of five highly virulent infectious bursal disease viruses (IBDVs) isolated in Japan were amplified by polymerase chain reaction (PCR) and sequenced. The nucleotide sequences of five highly virulent IBDVs were identical. Comparisons of the nucleotide and the deduced amino acid sequences with those of other strains of IBDV indicated that Japanese highly virulent IBDV is different from all other strains of IBDV that were compared. The number of amino acids that differed between strains (substitution score) showed that highly virulent IBDV is more closely related to European virulent strain 52/70 than to Japanese conventional strains. These results strongly suggest that a single strain of highly virulent IBDV that might have originated from a European strain is prevalent in Japan.

Amino Acid Sequence↗

Comparison of dynamic CT and stable xenon CT in ischemic cerebrovascular disease.

PURPOSE: To investigate a new hemodynamic parameter that can be obtained by dynamic CT and that reflects cerebral blood flow (CBF), in patients with ischemic cerebrovascular disease. METHODS: CBF and hemodynamic parameters including the area under the time-dependent contrast-medium dilution curve (A) and mean transit time (MTT) were measured in 23 patients with ischemic cerebrovascular disease. They included 17 patients in the chronic stage (more than 1 month after onset) and six with acute occlusion of the internal carotid or middle cerebral artery (within 24 hours of onset). CBF measurement was conducted by inhalation of stable xenon during CT scan and the hemodynamic study was performed using dynamic CT. RESULTS: CBF in the territory of the middle cerebral artery had an inverse correlation with MTT. (A) divided by (MTT) defined as (f) had a significantly positive correlation with CBF (MTT = 18.66 - 0.495.CBF + 0.005.CBF2, r = .730, P < .001). CONCLUSION: This parameter (f) is thought to represent a relative CBF and it can be used in evaluation of the hemodynamic status in ischemic cerebrovascular disease.

Aged↗

The effect of route of inoculation on protection by killed vaccines in chickens.

The effect of various routes of immunization on protection against challenge by virulent agents was examined in chickens. Chickens were immunized intratracheally, intranasally, per os, by crop gavage, and intramuscularly. Agents examined were killed Haemophilus paragallinarum, Mycoplasma gallisepticum, and infectious bursal disease virus. Results of immunization by intratracheal administration were equivalent to those produced by parenteral administration. All vaccines effectively induced production of serum antibodies against pathogens, and all immunized chickens were protected against infection by each pathogen.

Administration, Intranasal↗

Comparison of the effects of various anticholinergic drugs on human isolated urinary bladder.

We investigated the effects of various anticholinergic drugs (atropine, oxybutynin, terodiline and propiverine) on the contractions induced by acetylcholine, KCl, CaCl2, and electrical field stimulation, in human isolated urinary bladder smooth muscles using the muscle bath technique. Urinary bladders were obtained from 20 patients who underwent total cystectomy due to malignant bladder tumor. The detrusor preparations were taken from the intact part of the dome of the bladder. Acetylcholine caused a concentration-dependent contraction in human detrusor preparations. Atropine (10(-9)-10(-6) M), oxybutynin (10(-8)-10(-5) M), terodiline (10(-7)-10(-5) M) and propiverine (10(-7)-10(-5) M) caused parallel shifts to the right of the concentration-response curves to acetylcholine. The rank order of pA2 values was: atropine > oxybutynin > terodiline = propiverine. Atropine did not suppress the maximum contraction to acetylcholine, while the other drugs significantly suppressed the maximum contractions at the higher concentrations. Each drug caused a concentration-dependent inhibition of the KCl (80 mM)- and CaCl2 (5 mM)-induced contractions; the maximum inhibitions of terodiline and propiverine were significantly greater than those of oxybutynin and atropine. Each drug caused a concentration-dependent inhibition of the contraction induced by electrical field stimulation; the maximum inhibitions of terodiline and propiverine were significantly greater than those of oxybutynin and atropine. The results suggest that the drugs have both anticholinergic and calcium antagonistic effects. Furthermore, it also appears that part of the human bladder contraction, which was significantly inhibited by terodiline and propiverine, is an atropine-resistant component.

Acetylcholine↗

Two long-term survivors after microwave coagulation therapy for hepatocellular carcinoma: a case report.

We present two long-term survivors who underwent microwave coagulation therapy (MCT) for hepatocellular carcinoma (HCC). One patient was a 55-yr-old man having a solitary HCC of 25 mm in diameter associated with advanced liver cirrhosis. He underwent MCT instead of hepatic resection because he developed bleeding tendency during surgery. The other patient was a 78-yr-old woman having a 7 cm tumor in segment II and a 15 mm satellite lesion in segment IV. Because manipulation of the left hepatic lobe caused prolonged hypotension, only the main tumor was resected. The satellite lesion was treated with MCT. In both cases, tumors and surrounding liver parenchyma were widely coagulated. Postoperative courses were uneventful. The coagulated area shrank subsequently after surgery. Both patients are alive 39 and 55 months after MCT without tumor recurrence. This report proves that MCT is a curative treatment and an alternative to hepatic resection in selected patients with HCCs.

Aged↗