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Biomedical subjects

S U Kim

Publications and source records attributed to S U Kim.

At least 145 records · Page 8Linked to original sources
PubMed1992

Development of inositol 1,4,5-trisphosphate 3-kinase immunoreactivity in cerebellar Purkinje cells in vivo and in vitro.

Development profiles in vivo and in vitro of inositol 1,4,5-trisphosphate 3-kinase (IP3K) were investigated immunohistochemically in the cerebellar Purkinje cells. In in vivo preparations of rat cerebellum, IP3K immunoreactivity appeared in Purkinje cell bodies and dendrites shortly after birth, increased rapidly by postnatal day 5, and was subsequently confined to their dendritic processes by day 20. The appearance and shift of IP3K immunoreactivity in Purkinje cells showed an identical time course even when Purkinje cells were placed under culture conditions commencing on day 0, suggesting that Purkinje cells have their own biological clock on the expression of IP3K in the absence of external influences.

Animals↗
PubMed1992

Immunohistochemical study of A2B5-positive ganglioside in postmortem human brain tissue of Alzheimer disease, amyotrophic lateral sclerosis, progressive supranuclear palsy and control cases.

Localization of gangliosides positively stained by the monoclonal antibody A2B5 was investigated in postmortem brain tissue of Alzheimer disease, amyotrophic lateral sclerosis (ALS), progressive supranuclear palsy (PSP) and control cases. In control cases, A2B5-staining was granular, appearing in selective neuronal populations. In the neocortex, the A2B5-positive neurons were distributed mainly in deep cortical layers. In the cerebellum, A2B5-positive structures were detected in processes extending from the Purkinje cell layer into the molecular layer. In Alzheimer cases, many neurofibrillary tangles, neuropil threads and dystrophic neurites were strongly A2B5-positive. In addition, aggregations of A2B5-positive granules were detected in some neurons lacking neurofibrillary tangles. Alterations of A2B5-positive gangliosides were also detected in ALS and PSP cases. In ALS cases, A2B5-positive granules were aggregated in Betz cells of the precentral gyrus. In PSP cases, globose-type neurofibrillary tangles were also strongly A2B5-positive. The results indicate that A2B5-positive gangliosides are widely but selectively distributed in human brain and may be involved in several neuropathological processes.

Aged↗
PubMed1992

beta-Amyloid precursor protein of Alzheimer's disease in cultured bovine oligodendrocytes.

The production of beta-amyloid precursor protein (beta APP) in cultured oligodendrocytes isolated from adult bovine brains was examined by immunohistochemistry and immunoblotting. Immunostaining of oligodendrocytes with antibodies specific for the carboxy terminus of beta APP demonstrated positive immunoreactivity of oligodendroglial cytoplasm. Immunoblot analysis of cellular extracts detected two distinct bands with estimated molecular weight of 118 and 105 kDa. The amount of these beta APP subspecies increased considerably in response to their attachment to the poly-L-lysine substratum.

Alzheimer Disease↗
PubMed1992

Calbindin D-28k and parvalbumin immunohistochemistry in developing rat retina.

The developmental profiles of two calcium-binding proteins, calbindin-D28k (CaBP) and parvalbumin (PV), were investigated immunohistochemically in the developing rat retina. CaBP-immunoreactivity appeared first on embryonic day 17 in the horizontal, amacrine and ganglion cells; on embryonic day 21 in the inner plexiform layer; and on post-natal day 6 in the outer plexiform layer. The reaction intensity had increased to its maximum level by post-natal day 10. PV-immunoreactivity was first noted on embryonic day 19 in the amacrine and ganglion cells and reached its maximum on post-natal day 10. Two distinct subpopulations of amacrine cells were clearly recognized after post-natal day 6; one was positive for CaBP and the other for PV. Some morphological differences were noted between the two.

Animals↗
PubMed1992

Alzheimer's disease beta-amyloid precursor protein in rat neural cells in culture.

Immunochemical studies were performed on Alzheimer's beta-amyloid precursor protein (APP) in rat brain and cultured rat neural cells. Multiple APP subtypes were detected on immunoblots of brain homogenate with several antisera specific for subsequences of APP. In rat neural cell cultures, it was demonstrated that the composition of APP subtypes differed among cell types and subcellular fractions, and that APP subtypes in PC12h cells varied in their heparin binding affinity, suggesting distinct functional roles for different APP subtypes. Compatible with the possible role of APP in cell-matrix interaction, an increase in oligodendroglial APP was observed following their attachment onto poly-L-lysine substratum.

Alzheimer Disease↗
PubMed1991

Phospholipase C isozymes in neurons and glial cells in culture: an immunocytochemical and immunochemical study.

Expression of 3 brain isozymes of phospholipase (PLC-beta, PLC-gamma and PLC-delta) was investigated in relation to cell types found in rat CNS cultures. Immunoreactivity of cultured neurons, astrocytes and oligodendrocytes was demonstrated for all of the 3 isozymes by immunocytochemical staining and immunoblotting, with some differences in reaction intensity. Immunoblotting revealed that the level of expression was neurons greater than oligodendrocytes greater than astrocytes for PLC-beta and PLC-gamma, and astrocytes greater than oligodendrocytes greater than neurons for PLC-delta.

Animals↗
PubMed1991

Alpha 1-antichymotrypsin supports short-term survival of cerebral neurons in culture.

alpha 1-Antichymotrypsin (ACT) is a component of the amyloid deposits in Alzheimer's disease. To elucidate its biological activities in the central nervous system, dissociated cultures of rat cerebral neurons were grown in a serum-free medium containing ACT. The addition of ACT (0.1-1 microgram/ml) enhanced survival of microtubule-associated protein 2-immunoreactive neurons under the culture conditions examined.

Animals↗
PubMed1991

Developmental changes of three phosphoinositide-specific phospholipase C isozymes in the rat nervous system.

Developmental changes of 3 phosphoinositide-specific phospholipase C isozymes (PI-PLC-beta, PI-PLC-gamma and PI-PLC-delta) in the rat nervous system were studied by immunohistochemical and immunochemical methods. PI-PLC-gamma immunoreactivity was intensely expressed in the radial fibers from the late fetal to early newborn stages, while weaker PI-PLC-beta reaction was also demonstrated in these structures. PI-PLC-beta and PI-PLC-gamma immunoreactivity appeared in neurons of various regions after the first postnatal week and then increased to the adult stage. Bergmann glia and some astrocytes also showed weak immunoreactivity for both isozymes from the newborn stage, while such immunoreactive astrocytes were relatively restricted in distribution in the white matter and hippocampus at the adult stage. PI-PLC-delta immunoreactivity appeared in astrocytes of entire cerebral regions from the second postnatal week, although weak antigenicity was also present in some neurons. Immunoblot analysis revealed that the immunoreactivities of 3 PI-PLC isozymes were present in both fetal and adult brains, with strong reactions of PI-PLC-beta and PI-PLC-delta in adult brain and that of PI-PLC-gamma in fetal brain. These results suggest that each PI-PLC isozyme plays important roles in different cell types in the course of their differentiation, and that some PI-PLC isozymes, especially PI-PLC-gamma, may be involved in cellular division and growth during the histogenesis of the central nervous system.

Animals↗
PubMed1991

Ion channels in cultured adult human Schwann cells.

Single channel currents have been recorded from cultured adult human Schwann cells. In both cell-attached and -excised (inside-out) patches, openings from a high-conductance (360 pS) channel were observed; measurements of the zero-current potential indicated that the channel was predominantly selective for chloride. Depolarizing and hyperpolarizing voltage steps activated the anion channel, which subsequently reverted to a closed state even in the presence of the maintained step. A second channel, with a conductance near 20 pS and with a current amplitude that increased with patch hyperpolarization, passed inward K+ currents in both cell-attached and inside-out patches. The mean open times for this channel were near 20 ms at the cell resting potential and decreased with patch hyperpolarization. The presence of these anion and cation selective channels in the human Schwann cell membrane would be consistent with a role for the cells in the regulation of extracellular K+.

Anions↗
PubMed1991

Expression and induction of intercellular adhesion molecules (ICAMs) and major histocompatibility complex (MHC) antigens on cultured murine oligodendrocytes and astrocytes.

Expression of intercellular adhesion molecule-1 (ICAM-1), ICAM-2-like molecule (Lgp55), and class I/II major histocompatibility complex (MHC) antigens (H-2 and Ia) was investigated in cultures of murine oligodendrocytes and astrocytes. Under unstimulated conditions, low levels of ICAM-1 expression were observed on astrocytes (less than 20%), but not on oligodendrocytes. Lgp55 was expressed intensely on oligodendrocytes (greater than 90%) and to a lesser degree on astrocytes (greater than 70%). A weak class I MHC (H-2) immunoreactivity was identified on both oligodendrocytes and astrocytes (50-70%). Class II MHC (Ia) antigen was undetectable on both cell types. After 48-hr exposure to immune mediators that include interferon-gamma (IFN-gamma), 500 U/ml, and supernatant from concanavalin A (Con A)-activated spleen cells, ICAM-1 expression was markedly increased on astrocytes (greater than 80%), but not on oligodendrocytes. Lgp55 expression on both cell types was not altered. Induction of H-2 antigen expression by immune mediators was quite high on both cell types (greater than 95%), while Ia antigen induction was low on astrocytes (less than 50%) and did not occur on oligodendrocytes. Cell type-specific expression and induction of ICAMs and MHC antigens by immune mediators may play roles in lymphocyte-glial cell interactions at the sites of inflammation in the central nervous system (CNS).

Animals↗
PubMed1991

Age-dependent decrease of process formation by cultured oligodendrocytes is augmented by protein kinase C stimulation.

The proportion of cultured rat oligodendrocytes (OL) that extended processes of over three soma diameter in length is dependent on the age of the animals from which the brains were derived; up to 70% of neonatal OL attained this criterion within 3 days, and this proportion progressively decreased with advancing ages of the animals (1, 3, and 6 months). The lower extent of process formation from older rat OL could be augmented, and indeed to equal neonatal levels, by treatment of cells with phorbol esters that stimulate protein kinase C: 4 beta-phorbol-12,13-dibutyrate (PDB) and phorbol-12-myristate-13-acetate (PMA). Enhancement of process formation by PDB and PMA was also observed for cultured adult human and bovine OL. For adult OL from all three species, a phorbol ester that binds but that does not activate protein kinase C, 4 alpha-phorbol-12,13-didecanoate, did not result in enhancement of process formation. Selectively to biologically active phorbol esters was shown by the inability of a wide range of growth factors to promote process extension. Immunohistochemical analyses indicate that the type III isozyme of protein kinase C predominates in cultured OL; the apparent intensity of immunoreactive PKC was not different between controls or cultures treated for 12 days with PDB, suggesting that the persistent presence of PDB might not have down-regulated the enzyme, in contrast to other cell types. We propose that stimulation of protein kinase C is critical to the triggering of process formation by cultured OL in vitro.

Aging↗
PubMed1991

Oligodendroglial cell death induced by oxygen radicals and its protection by catalase.

The cytotoxic effects of oxygen radicals have been studied in enriched population of mature bovine oligodendrocytes in culture. Oxygen radicals were generated enzymatically by glucose and glucose oxidase, and hypoxanthine and xanthine oxidase combinations. Cytotoxicity was assessed by trypan blue exclusion and percentage lactate dehydrogenase release into the culture media. Incubation of bovine oligodendrocytes with these oxygen radical-generating systems for 4 hr resulted in significant cell death, especially in the glucose oxidase system. The oligodendrocytes were completely protected by catalase from the cytotoxic effects of both oxygen radical generating systems. However, superoxide dismutase, dimethylsulfoxide and antioxidants such as vitamin E and glutathione did not protect oligodendrocytes from the oxidant-mediated cytotoxicity. It appears that hydrogen peroxide produced in these oxygen radical-generating systems gives rise to toxic radicals that induce the cell death of bovine oligodendrocytes in culture.

Animals↗
PubMed1991

Neonatal mammalian spinal cord neurons and motoneurons in monolayer culture.

Previously, embryonic tissues have been used to produce monolayer cultures containing mammalian spinal cord neurons (SCN) and motoneurons (MN) for studies of the pathophysiology of motoneuron diseases. We demonstrate here that viable SCN and MN were observed in dissociated cultures from neonatal rat and mouse. These SCN and MN produced neurites and expressed acetylcholinesterase, neuron-specific enolase and neurofilament protein. These results indicate that cultured postnatal SCN and MN are capable of survival, neurite extension, and phenotypic expression in culture.

Animals↗
PubMed1991

Lymphokine-activated killer (LAK) and adherent LAK (A-LAK) activity in multiple sclerosis.

The cytotoxic activity of lymphokine-activated killer (LAK) cells against enriched cultures of oligodendrocytes (OL) was investigated in multiple sclerosis (MS) and controls. Human LAK cells, derived from macrophage-depleted peripheral blood mononuclear cells (PBMC) and incubated with recombinant human interleukin-2 (IL-2) (20-80 U/ml), mediated high levels of cytotoxicity against Raji cells but low levels of cytotoxicity against primary cultures of bovine OL. Cytotoxicity was not enhanced by incubation with a high level of IL-2 (500 U/ml). No statistically significant differences in LAK cell activity against bovine OL were observed among the study groups. Enriched adherent LAK (A-LAK) cells mediated greater levels of cytotoxicity against both bovine OL and tumor cell lines than unfractionated LAK cells. Flow cytometric analysis indicated that A-LAK effector cells were CD4-, CD8+, and CD16+. Furthermore, A-LAK cells mediated lysis of OL derived from several different animal species. Our results suggest that LAK and A-LAK cells can mediate cytolysis of OL in culture similar to that observed with a number of different tumor cell lines. However, no significant difference in cytolysis was identified between MS and control groups in this study.

Adult↗
PubMed1991

Cytolysis of oligodendrocytes is mediated by killer (K) cells but not by natural killer (NK) cells.

The cytotoxic activity of killer (K) cells against enriched cultures of bovine oligodendrocytes (BOL) was investigated in multiple sclerosis (MS) and controls. Human K cells mediated cytotoxicity to primary cultures of BOL in the presence of anti-BOL antiserum in all study groups, while BOL were resistant to human natural killer (NK) cells. Cytotoxic activity was significantly reduced in MS when compared to age-matched normal controls but not when compared to other neurologic disease (OND) patients. K cell-mediated lysis of BOL could also be induced with anti-galactocerebroside antibody but not with other antibodies including those specific for OL antigens (myelin basic protein, proteolipid apoprotein, and 2',3'-cyclic nucleotide 3'-phosphodiesterase). Enrichment of the effector population indicated that antibody-dependent cell-mediated cytotoxicity (ADCC) to BOL was mediated by large granular lymphocytes, and the effector population was further characterized by flow cytometry. The effector cells mediating ADCC could be inhibited by protein A of Staphylococcus aureus, and by K562 cells in cold competition assay. These observations indicate that oligodendrocytes are resistant to NK cells but are susceptible to cytolysis mediated by K cells. This may represent a potentially important immune mechanism in the pathogenesis of MS.

Adult↗
PubMed1991

Psychosine cytotoxicity toward rat C6 glioma cells and the protective effects of phorbol ester and dimethylsulfoxide: implications for therapy in Krabbe disease.

Psychosine cytotoxicity was tested as to its effects on rat C6 glioma cells. At a low concentration--below 40 microM--psychosine appeared to stimulate cell proliferation. Above the concentration range of 40 microM-60 microM, however, it showed a cytotoxic effect. When phorbol ester (PDB) or dimethylsulfoxide (DMSO) was supplemented to cultures being exposed to psychosine, the total number of live cells, protein content and CNPase activity dramatically increased as compared with the levels in cultures treated with psychosine alone. The results of these basic studies suggest another approach as to therapy for globoid cell leukodystrophy.

2',3'-Cyclic-Nucleotide Phosphodiesterases↗
PubMed1991

Expression and modulation of HLA-DR on cultured human adult astrocytes.

Expression of Class II major histocompatibility complex (MHC) antigens on astrocytes has been implicated as contributing to the immune responses characteristic of chronic autoimmune diseases of the central nervous system. We examined the properties and regulation of HLA-DR on cultured human adult astrocytes. We found that a proportion of human astrocytes from each of fifteen individual donors expressed HLA-DR under basal culture condition; while this proportion differed among the human subjects (range 3-65%), the results for each individual remained relatively constant when analyzed at several time points (up to 125 days in vitro). Attempts to modulate HLA-DR expression by a variety of cytokines likely to be present in inflammatory infiltrates in the brain showed that only gamma-interferon could increase the proportion of human astrocytes that expressed HLA-DR. Whether the variability of HLA-DR expression on astrocytes between different individuals reflects a genetic trait which can influence susceptibility to autoimmune central nervous system diseases remains to be determined.

Adult↗