Biomedical subjects

S Tsugane

Publications and source records attributed to S Tsugane.

At least 73 records · Page 4Linked to original sources

PubMed1998

Prognostic significance of accumulation of gene and chromosome alterations and histological grade in node-negative breast carcinoma.

The histological grade of atypia is a known prognostic indicator for breast cancer patients and correlates with many gene and chromosome alterations. To investigate the independent prognostic significance of gene and chromosome alterations in axillary node-negative (n0) breast cancers of the invasive ductal and invasive lobular types, the prevalence of eight gene and chromosome alterations and their association with histological grade and recurrence was studied in 129 consecutive patients who had undergone resection over an average follow-up period of 43.4 months. Loss of heterozygosity on 16q, 17p, 16p, 17q and 18q, p53 gene mutation and c-erbB-2 and int-2 gene amplifications were detected in 55%, 37%, 25%, 24%, 22%, 23%, 15% and 11% respectively. Individual alterations in 17p, 17q, 18q, c-erbB-2 and p53 were detected most frequently and gene and chromosome alterations tended to be accumulated in Grade 3 n0 invasive ductal/lobular carcinomas. Histological grade, 18q, int-2 and the number of these gene and chromosome alterations were significant prognostic indicators on Cox's univariate proportional hazard model, and 18q and int-2 were still significant after adjustment for histological grade. Results suggested that examination of the presence of certain gene and chromosome alterations, as well as histological grade, were effective in identifying n0 breast cancer patients at high risk of early recurrence.

Breast Neoplasms↗

PubMed1998

Relationship of food intake and dietary patterns with blood pressure levels among middle-aged Japanese men.

To investigate the relationship of food intake habits and dietary patterns to blood pressure, a cross-sectional study was conducted for 473 middle-aged Japanese males. After adjustment for age, residence, occupation, body mass index and alcohol consumption, mean systolic and diastolic blood pressure (SBP and DBP) were inversely associated with each intake frequency of dairy products, coffee, fruits, egg, beef, pork and chicken. The adjusted mean SBP and DBP of the individuals with 'all (= three)' of the following three dietary habits; 'once and over/wk of dairy products', 'once and over/wk of fruits', and 'three times and over/wk of beef, pork or chicken', were 7.4 mmHg and 6.9 mmHg lower (p < 0.001, for each) than those of 'zero or one' group. According to the analyses on 3-days weighed food records of 157 volunteers, the adjusted mean daily intake of total protein, animal protein and potassium were markedly higher in the 'two' or the 'three' group than in the 'zero or one' group (p < 0.05, for each). These results suggest that habitual intake of dairy products, fruits, and meat or chicken may be associated with the reduction of blood pressure possibly through the intake of protein and potassium.

Adult↗

PubMed1998

Relationship of occupation to blood pressure among middle-aged Japanese men--the significance of the differences in body mass index and alcohol consumption.

To clarify how and why blood pressure differs between occupations, the proportions of hypertensives, and the measures of blood pressure, body mass index (BMI) and alcohol consumption among the individuals not taking antihypertensive drugs were compared between the eight occupational categories using the data from a health check-up for 589 middle-aged Japanese males, mostly randomly selected from five areas in Japan. After adjusting for age, the relationships of occupation to the proportion of hypertensives and the mean systolic and diastolic blood pressure substantially differed among the five areas. However, after further adjustment for residence, these blood pressure levels (the proportion of hypertensives, and the mean systolic and diastolic blood pressure) were found to be higher for the "Personnel in transport and communications", the "Clerical personnel", the "Managerial and civil personnel" and the "Professional and technical personnel", whereas these values were consistently lowest in the "Service personnel". Age and residence-adjusted mean BMI was also higher for the four occupational categories with the increased blood pressure levels. According to a weighted multiple regression analysis across the eight occupations, the age and residence-adjusted mean BMI was a significant predictor of the age and residence-adjusted mean systolic and diastolic blood pressure (p = 0.068 and 0.018, respectively). These results suggest that the occupation-related changes in BMI may largely contribute to the occupation-related changes in blood pressure.

Alcohol Drinking↗

PubMed1998

Association of Ile462Val (Exon 7) polymorphism of cytochrome P450 IA1 with lung cancer in the Asian population: further evidence from a case-control study in Okinawa.

Okinawa, a group of islands that lie between the East China Sea and the Pacific Ocean, 2000 km south of the Japanese main islands, has a different profile of diseases, ethnicities, and cultures than does the rest of Japan. We examined an Ile462Val polymorphism (CYP1A1*2 allele) of cytochrome P450 IA1 in a hospital-based case-control study of lung cancer patients (247 cases and 185 controls) in Okinawa to ascertain the association of this variant with lung cancer. In addition, the distribution of this genotype was studied in populations from different areas of Japan, including Tokyo (n = 69) and Iwate (northern part of Japan; n = 81), as well as in a Chinese group from the Jiangsu province (n = 39) and in an Australian Caucasian group (n = 146). Genotype frequency in controls was not significantly different from area to area in Japan. In Okinawa, however, the genotype encoding Val/Val was associated with a significantly higher risk of lung cancer (odds ratio = 3.32, P = 0.013), especially of squamous cell carcinoma and small cell carcinoma (odds ratio = 4.85 and 9.35, respectively). The Val-encoding allele was less frequent in the Chinese population and was rare in Australian Caucasians. Thus, this study gives support to the value of the cytochrome P450 IA1 Ile462Val polymorphism as a practical high-risk marker of lung cancer in populations, especially those in southeast Asia, in which this variant is more common.

Adult↗

PubMed1997

Different effects of hypertension and hypercholesterolemia on the natural history of aortic atherosclerosis by the stage of intimal lesions.

To investigate the histopathologic modes of the effect of hypertension and hypercholesterolemia on atherosclerosis progression, a total of 573 male autopsied aortas, ranging from 0 to 97 years-old, were histomorphometrically compared by the status based on antemortem risk factors. Specimens were classified into four categories according to the criteria reported by the American Heart Association after histometric measurement at defined sites of the aortas. Intimal lesions progressed in the same fashion in all the risk factor groups examined; normal intima converted to fatty streak, preatheroma (characterized by microscopic extracellular lipid deposition) and then atheroma. This progression of intimal lesions correlated with age-related increases in intimal thickness independent of risk factors. Although the frequency of fatty streaks and the population of foam cells were greater in the hypercholesterolemics than in the non-risk patients, the frequencies of preatheroma and atheroma were not different between these two patient groups until patients reached the fifth decade. In contrast, the frequencies of preatheroma and atheroma were consistently greater in the hypertensives than in the other groups by the fifth decade. Hypertension was also related to intimal thickness in the younger groups. Our findings suggest that hypertension and hypercholesterolemia affect the progression of atherosclerosis differently by histopathologic stages.

Adolescent↗

PubMed1997

A randomized trial of surgery with and without chemotherapy for localized squamous carcinoma of the thoracic esophagus: the Japan Clinical Oncology Group Study.

OBJECTIVE: To determine whether postoperative adjuvant chemotherapy confers a survival benefit on patients with esophageal squamous cell carcinoma undergoing radical surgery, we undertook a cooperative, prospective randomized controlled trial. METHODS: A total of 205 patients underwent transthoracic esophagectomy with lymphadenectomy at eleven institutions between December 1988 and July 1991. These patients were prospectively randomized into two groups (100 patients underwent surgery alone and 105 patients had additional two courses of combination chemotherapy with cisplatin (70 mg/m2) and vindesine (3 mg/m2). The two groups did not differ with respect to sex, age, location of tumor, and distributions of pT, pN, pM, or p stage. RESULTS: The 5-year survival was 44.9% in the surgery alone group and 48.1% in the surgery plus chemotherapy group. The relative risk was estimated to be 0.89 (95% confidence interval, 0.61 to 1.31) in the surgery plus chemotherapy group compared with the surgery alone group. No significant differences in survival were detected between the two groups, even with lymph node stratification. CONCLUSION: Postoperative adjuvant chemotherapy with cisplatin and vindesine has no additive effect on survival in patients with esophageal cancer compared with surgery alone.

Adult↗

PubMed1997

Green tea intake in relation to serum lipid levels in Middle-aged Japanese men and women.

PURPOSE: The relationship between green tea intake and serum concentrations of total cholesterol, triglycerides, and high density lipoprotein cholesterol was examined. METHODS: The subjects were 630 middle-aged men and their 370 wives sampled from five regions of Japan during 1989-1991. Consumption frequency of 38 foods, including green tea, was determined by interview. Three-day food records were collected from 207 of the men and 164 of the wives. The mean serum concentrations of the three lipids were compared according to the three levels of daily green tea intake (< 1 cup, 1-4 cups, and > 4 cups), with adjustments for various health habits, food frequencies, and nutrient intakes. RESULTS: After extensive multivariate adjustments for nondietary and dietary covariates, green tea was not associated with any of the lipid levels. CONCLUSIONS: The results of this cross-sectional study do not support the beneficial effects of green tea on serum lipid levels.

Adult↗

PubMed1997

Food consumption and gastric cancer mortality in five regions of Japan.

To explore the causes of the threefold variation in mortality rate from gastric cancer in Japan, we studied the geographic correlations between food consumption and age-adjusted mortality rates in five regions, which covered the areas with the highest and lowest mortality rates in the country. Six hundred thirty-four men and wives of 373 of the men sampled from the general populations in these districts were interviewed about intake frequency of 38 food items. Age-adjusted mean frequencies of food intake differed markedly among the regions, in which 32 food items for men and 28 for women showed a significant difference (p < 0.05). Rank correlation coefficients between the average frequencies and the mortality rates with adjustment for sex and prevalence of current smokers were 0.49, 0.32, 0.36, -0.88, and -0.57 for rice, bean paste soup, pickled vegetables, green vegetables, and yellow vegetables, respectively. These results suggest that the regional differences in consumption of these foods may account partly for the geographic variation in gastric cancer mortality in Japan.

Adult↗

PubMed1997

Nutrient consumption and gastric cancer mortality in five regions of Japan.

To explore the causes of the threefold variation in mortality rate from gastric cancer in Japan, we studied the geographic correlations between nutrient consumption and the disease in five Public Health Center districts including the regions with the highest and lowest mortality rates in the country. In the winters of 1989-1991, a three-day weighed food record was collected from 207 men and the wives of 165 of the men sampled from the five districts. The average daily consumption of selected nutrients was computed and correlated with the age-adjusted mortality rates from gastric cancer. Partial rank correlation coefficients adjusted for sex and other nutrients were 0.45, -0.80, -0.20, and -0.07 for sodium, carotene, ascorbic acid, and retinol, respectively. The results suggest that variation in gastric cancer mortality in Japan may be partly accounted for by the regional differences in consumption of sodium, carotene, and possibly ascorbic acid.

Adult↗

PubMed1997

Within- and between-person variations in portion sizes of foods consumed by the Japanese population.

There has been considerable controversy about the utility of asking separate questions on portion sizes in addition to items on consumption frequencies in food frequency questionnaires. One argument against this type of inquiry is that, for most foods, within-person variation of portion size is greater than between-person variation, making it difficult to characterize "usual" or long-term average serving sizes. The authors studied the relative effects of within- and between-person variances in portion sizes of 69 food items using three-day food record data collected from 335 Japanese men and women. Total variance in the portion size for each food was partitioned into within- and between-person components by analysis of variance. Although the ratios of within- to between-person variances in log(e)-transformed portion sizes were > 1.0 for the majority of foods (50 of 69 items, median = 1.7), they were smaller than or close to unity for subgroups of foods that were the major sources of nutrient variables of relevance to diet-cancer associations, such as total energy (rice, calcium (milk), sodium (pickled vegetables), and alcohol (beer, shochu, whiskey). These results indicate that the relative contributions of within- and between-person variations in portion size may vary among foods, and therefore investigators should consider the balance between the advantages and disadvantages of obtaining portion size data separately from intake frequencies when designing food frequency questionnaires.

Adult↗

PubMed1997

Modelling of mortality data from a multi-centre study in Japan by means of Poisson regression with error in variables.

BACKGROUND: Death rates of particular categories in epidemiological studies are often based on a small number of occurrences which can be well described by a Poisson distribution. METHOD: We applied this model for the analysis of a multi-centre study in five Japanese counties where the death rates of stomach cancer (ICD-9 code 151) in four age groups are known. In our example some covariates of the cases (e.g. plasma lycopene levels) are unknown values and are estimated from a randomly chosen collective. Therefore these values are subject to a sampling error. The inclusion of errors in variables (e-i-v) into the statistical model can adequately describe such a situation. The model is estimated in a Bayesian framework by means of resampling techniques. RESULTS: Based on the posterior distribution of the parameters the relative risk of stomach cancer is 0.46 (95% confidence interval: 0.23-0.79) comparing the maximum of the population medians of lycopene with the minimum. The estimated overdispersion is close to zero indicating only minor interference with other possible explanatory variables. In addition, we show that inclusion of e-i-v can give more accurate estimates of the parameters even from small sample sizes. CONCLUSIONS: Appropriate statistical methods allow the accurate estimation of relative risks from small sample sizes and from low number of cases. Lycopene plasma levels are good predictors for stomach cancer.

Adult↗

PubMed1997

Histologic grade and p53 immunoreaction as indicators of early recurrence of node-negative breast cancer.

Even among breast cancer patients without metastasis to axillary lymph nodes, early recurrence can occur. To determine the risk factors for early recurrence, we performed a case-control study between 32 patients with an early recurrence of breast cancer and 122 patients without recurrence, in which tumor size, age of patient and date of operation were matched. In all 154 node-negative patients, followed up over a 13.1-year median period, expression of p53, c-erbB-2 and cathepsin D in the primary tumor were studied immunohistochemically in paraffin-embedded tissues and were compared with morphologic factors such as histologic grade and invasive growth. Univariate analysis showed that nuclear p53 immunoreaction was significantly predictive of early recurrence [risk ratio (RR) 3.3]. Likewise, cathepsin D (RR 0.22) was significantly negatively associated; however, when the risk ratio was analyzed in terms of intensity of cathepsin D staining, no superior survival was found for patients with strongly positive tumors. Overexpression of c-erbB-2 protein was not associated with outcome by either univariate or multivariate analysis. As a whole, histologic grade was confirmed as being a strong predictor (RR 42.6) and multivariate analysis showed that only histologic grade was a significant risk factor for early recurrence. p53 immunoreaction was not a significant independent factor because it was closely linked to histologic grade (P = 0.002), especially to a high mitotic index (P < 0.001). Moreover, in 14 patients with "special histologic types' of invasive carcinomas and no recurrence, all were p53-negative except one medullary carcinoma. Nuclear p53 immunoreaction is useful in supporting histologic grade to detect a high-risk for early recurrence in node-negative patients who may be eligible for systemic adjuvant therapy.

Adult↗

PubMed1997

A randomized controlled trial for chemoprevention of gastric cancer in high-risk Japanese population; study design, feasibility and protocol modification.

We have initiated a population-based, double-blind, randomized controlled trial to examine the effects of supplementation of beta-carotene and vitamin C on the incidence of gastric cancer. The subjects were participants in an annual health screening program conducted by four municipalities in Akita prefecture, one of the regions with the highest mortality from gastric cancer in Japan. We measured their serum levels of pepsinogens (PGs) I and II, and asked persons diagnosed with chronic atrophic gastritis (defined as PG I < 70 ng/ml and PG I/PG II ratio < 3.0) to take diet supplements containing 0 or 15 mg/day beta-carotene and 50 or 500 mg/day vitamin C for 5 years. During the first year of recruitment conducted in one village from June through September, 1995, 52% (635/1214) of screening participants had chronic atrophic gastritis and 73% (439/602) of eligible persons responded. However, in response to a National Cancer Institute press report released on January 18, 1996, indicating that two beta-carotene trials had shown no benefit and potential harm from the supplement, we discontinued the beta-carotene and continued with the trial using only vitamin C. Of 397 participants remaining at this point, 77% (305) consented to stay in the study. The results indicate that a randomized controlled trial for cancer prevention is feasible in the Japanese asymptomatic population.

Adult↗

PubMed1997

Drinking habit as a base for blood pressure elevation--difference in epidemiological significance by beverage type.

To investigate whether blood pressure differs by taking preferred alcoholic beverage among habitual drinkers, systolic and diastolic blood pressure (SBP, DBP) were compared among groups with different beverage types in 563 middle-aged Japanese males using data from a cross-sectional health survey conducted from February, 1989 through March, 1991 in five areas of Japan. Mean values of SBP and DBP, adjusted for residence, age and body mass index (BMI), were significantly greater in 'exclusively sake' drinkers (adjusted SBP: 127.2 mmHg, adjusted DBP: 83.0 mmHg) and in 'exclusively shochu' drinkers (adjusted SBP: 127.5 mmHg, adjusted DBP: 84.2 mmHg) than in non-drinkers (adjusted SBP: 120.9 mmHg, adjusted DBP: 77.3 mmHg). Adjusted SBP and DBP of 'exclusively beer' drinkers (adjusted SBP: 121.9 mmHg, adjusted DBP: 79.1 mmHg) were significantly (for SBP: p = 0.016, for DBP: p = 0.008) lower than those of 'exclusively sake' drinkers. Similar patterns of blood pressure differences between five beverage types of habitual drinkers were found especially in the group with less than 150 g of weekly ethyl-alcohol consumption. Even after adding ethyl-alcohol consumption as a covariate among 479 habitual drinkers, the significant differences in adjusted SBP and DBP between 'exclusively beer' drinkers and 'exclusively sake' drinkers (for SBP: p = 0.032, for DBP: p = 0.044) were noted. These results may suggest that the effects of drinking on blood pressure differ by beverage type in middle-aged Japanese males.

Adult↗

PubMed1997

Dietary patterns in Japanese migrants to southeastern Brazil and their descendants.

This paper describes the dietary patterns of people of Japanese ancestry living in São Paulo, Brazil. Two cross-sectional surveys using a food frequency questionnaire (in 1989 and 1995) and self-administered three-day food record (only in 1995) were carried out in randomly chosen first-generation (Japan-born) and second-generation (Brazil-born) Japanese living in the city of São Paulo (n = 166), aged 40-69 years at the time of the first survey (1989). Daily intake of rice, bread, milk, fruits and coffee, and infrequent consumption of pork, green tea, black tea, tsukemono (pickled vegetables), seaweed and mushrooms were reported. The mean (+/- standard deviation) daily proportions of energy from fat among Japan-born participants were 27.2 +/- 6.7% for men and 26.2 +/- 6.7% for women. The respective figures for Brazil-born Japanese were 30.1 +/- 7.4% and 29.5 +/- 6.4%. These values were quite close to recent estimates for the general Brazilian population in metropolitan areas (about 30%), but seem to be higher than available data from Japan (25.3%). Dietary changes in this migrant population are discussed with focus on nutrients currently implicated in the etiology of major chronic diseases.

Aged↗

PubMed1996

Vasodilation by intrathecal lipopolysaccharide of the cerebral arteries after subarachnoid haemorrhage in dogs.

To investigate the influence of inducible nitric oxide synthase on cerebral arteries after subarachnoid haemorrhage (SAH) in vivo, lipopolysaccharide (LPS), a major inducer of inducible nitric oxide synthase, was injected intracisternally into control and SAH model dogs. Intracisternal injection of LPS (0.5 mg) produced a long-lasting, submaximal vasodilation of the basilar artery of control dogs on angiography. This effect became significant at 4 hours after LPS injection and plateaued after 6 hours. This vasodilation was reduced by N(G)-monomethyl-L-arginine. Vasopressin slightly suppressed the vasodilation, while bradykinin increased it. The concentration of L-arginine in CSF decreased after LPS injection, while that of L-citrulline increased. In cytokines, the concentration of tumour necrosis factor-alpha; (TNF-alpha;) in CSF increased transiently at 4 hours after LPS injection, while interleukin-1 beta, interleukin-6, interferon-gamma, did not change. These data suggest that vasodilation by LPS is mainly due to nitric oxide predominantly synthesized by an inducible nitric oxide synthase, proximally induced by TNF-alpha. Our data make it unlikely that SAH itself induces the inducible nitric oxide synthase in vascular tissue, since isolated endothelium-denuded basilar artery from SAH model dogs did not respond to L-arginine. In SAH model dogs, the degree of vasodilation by LPS differed with the severity of vasospasm. Vasodilation was much greater in mild than in severe vasospasm in dogs, and was increased by superoxide dismutase. These findings suggest that the induction of inducible nitric oxide synthase or its activity may be less effective in severe vasospasm.

Analysis of Variance↗

PubMed1996

Effects of human diets of two different Japanese populations on cancer incidence in rat hepatic drug-metabolizing and antioxidant enzyme systems.

Hepatic enzyme systems of drug metabolism and antioxidation were investigated in rats fed the complete human diets consumed in the two Japanese prefectures, Akita and Okinawa, where the incidence of cancers was quite different: Okinawa had the lowest and Akita the highest age-adjusted mortality rate. In rats fed the human diet consumed in Okinawa, hepatic glutathione S-transferase activity was higher and lipid peroxide content was lower than in rats fed the diet consumed in Akita. These data might indicate that the number and/or quantity of the dietary components attributed to the detoxification of carcinogens and the scavenging reactive carcinogen species was much higher in the foods consumed in the population having lower cancer mortality rate.

Animals↗

PubMed1996

Differential effects of cigarette smoking and alcohol consumption on the plasma levels of carotenoids in middle-aged Japanese men.

Although several studies have reported that cigarette smoking and alcohol consumption are inversely associated with the plasma level of beta-carotene even after adjustment for dietary carotene intake, their effects on other carotenoids have not been examined extensively. The authors examined the associations of smoking and alcohol with plasma levels of the five principal carotenoids (beta-carotene, alpha-carotene, lutein, lycopene, and zeaxanthin). The subjects were 634 apparently healthy men aged 40-49 years who were sampled randomly from five areas in Japan with varying rates of mortality from gastric cancer. Multiple linear regression analysis controlling for age, serum total cholesterol, serum triglycerides, body mass index, and consumption frequencies of green vegetables, yellow vegetables and fruit, showed that both smoking and alcohol had a significant inverse association with the plasma levels of beta-carotene and alpha-carotene; only smoking reduced the level of lutein, and neither smoking nor alcohol significantly affected the level of lycopene or zeaxanthin. With regard to the reduction of beta-carotene and alpha-carotene, the effect of smoking was smaller in drinkers than in nondrinkers, and the effect of alcohol was smaller in smokers than in nonsmokers, and significant interactions between smoking and alcohol were observed (P=0.034 for beta-carotene and 0.026 for alpha-carotene). The results indicate that the differential effects of smoking and alcohol should be considered when the health effects of plasma carotenoids are examined.

Adult↗