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Biomedical subjects

S Terada

Publications and source records attributed to S Terada.

At least 73 records · Page 4Linked to original sources
PubMed1997

Effects of hormone replacement therapy on serum amyloid P component in postmenopausal women.

The pentraxin serum amyloid P component (SAP) is a 9.5Sz1-glycoprotein and it has recently been found to be deposited in atherosclerotic lesions or neurofibrillary tangles, which are related to the aging process and Alzheimer's disease. The level of SAP was measured by micro single radial-immunodiffusion. Sample sera were obtained from 420 healthy humans, from newborn to 86 years old. The changes in SAP during the menstrual cycle were investigated in 6 women that were 20-21 years. Fifty of the postmenopausal women, suffering from climacteric symptoms, were administered either conjugated estrogen (E), or dehydroepiandrosterone (DHEA). The SAP levels increased with age, being 1.12 +/- 0.82 mg/dl (means +/- S.D.) in neonates, and 6.15 +/- 0.92 mg/dl in persons over 80 years. The SAP level in the females between 15 and 49 years (3.32 +/- 0.95 mg/dl) was significantly (P < 0.001) lower than that in the males in the same age group (5.19 +/- 1.25 mg/dl). The SAP level in the follicular phase was significantly (P < 0.01) lower than that in menstrual phase (menstrual: 4.36 +/- 0.90 mg/dl versus follicular: 2.61 +/- 0.99 mg/dl). In the post-menopausal women that were administered E (1.25 mg/day), the SAP decreased significantly (P < 0.001) from the prelevel of 5.64 +/- 1.40 mg/dl to 4.26 +/- 0.98 mg/dl on the 14th day. In the postmenopausal women that were administered DHEA (60 mg/day), the SAP increased rapidly from the prelevel of 4.97 +/- 0.76 mg/dl to 6.17 +/- 1.20 mg/dl on the 21st day. SAP seems to be a marker that can monitor the effect of hormone replacement therapy.

Adolescent↗
PubMed1997

The influence of observer calibration in temporomandibular joint magnetic resonance imaging diagnosis.

OBJECTIVE: This study was undertaken to investigate the potential of reducing observer variation through a calibration program. STUDY DESIGN: The study was based on three sets of randomly selected temporomandibular joint magnetic resonance images. Each set consisted of bilateral images from 20 consecutive patients with temporomandibular disorders. As a baseline, three well-experienced noncalibrated investigators interpreted the images individually for disk position and disk configuration. After the initial interpretation, interobserver agreement was calculated as a kappa index and presented to the examiners. On the same occasion, the investigators analyzed agreement between them on the criteria to be used. RESULTS: Overall data in this study showed an increase in the frequency of interobserver agreement with regard to disk position after the calibration trials were instituted. With regard to disk configuration, substantial interindividual variations were observed even after the observers reached consensus as to the criteria to be used. CONCLUSIONS: These data suggest that after calibration trials, it is possible for three examiners to obtain reliable and reproducible results in reporting temporomandibular joint disk position on magnetic resonance images.

Adult↗
PubMed1997

Effects of dehydroepiandrosterone and other sex steroid hormones on mammary carcinogenesis by direct injection of 7,12-dimethylbenz(a) anthracene (DMBA) in hyperprolactinemic female rats.

The present study was performed to investigate the effects of dehydroepiandrosterone (DHEA) compared with those of sex steroid hormones on the mammary tumor induced by local injection of 7,12-dimethylbenz(a) anthracene (DMBA) in hyperprolactinemic female rats. Under sustained hyperprolactinemia induced by pimozide (PMZ) from day 21, DMBA was injected locally into the mammary glandular tissues on day 73. Rats were divided into 5 groups as follows; steroid free (DP group), 17 beta-estradiol (DP + E2 group), testosterone (DP + T group), progesterone (DP + Prog group), or dehydroepiandrosterone (DP + DHA group). The growth pattern and histological classification of the tumor in these 5 groups and rats treated only with DMBA (D group) were examined. All of the tumors grew to a size of 10 mm in diameter and after retaining the size for a certain duration, increased the size rapidly again (onset of rapid tumor growth). The period from the day of DMBA administration to that of onset of the rapid tumor growth in DP group was shorter than in D group, and the period in DP + DHA was longer than DP group and longest in steroid-treated groups. The incidence of adenocarcinoma was 2 tumors/16 animals in D group, 9/11 in DP group, 5/11 in DP + Prog group, 2/7 in DP + E2 group, 2/8 in DP + T group, and 0/10 in DP + DHA group. The incidence of adenocarcinoma in each steroid group except in DP + Prog group was lower than in DP group. These results suggest that prolactin (PRL) increases the incidence of adenocarcinoma in the DMBA-induced mammary tumor model, and DHEA especially decreases the incidence of adenocarcinoma.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1997

Anti-apoptotic genes, bag-1 and bcl-2, enabled hybridoma cells to survive under treatment for arresting cell cycle.

Hybridoma 2E3-O cells were transfected with bcl-2 alone or with bcl-2 and bag-1 in combination. The bcl-2/bag-1 transfectant survived maintaining viability above 75% for almost 5 days when the cells were treated with excess (30 mM) thymidine for arresting cell cycle, whereas the mock transfectant survived for only 2 days, and the bcl-2 alone transfectant lived for 4 days. Owing to this extended viable culture period, the bcl-2/bag-1 transfectant produced twofold amount of antibody in comparison with the mock transfectant in non-proliferating state prepared by the excess thymidine treatment. When their proliferation was arrested by serum limitation, the bcl-2/bag-1 transfectant and the bcl-2 alone transfectant survived for 3 days maintaining viability above 75% while the mock transfectant survived only 1 day. The bcl-2/bag-1 transfectans produced the antibody at the rate three times as high as the bcl-2 alone transfectant and the mock transfectant in non-proliferating state established by serum limitation. Such genetic engineering of hybridoma cells for improving survival in the non-proliferating state will be useful for using nutrients in culture medium efficiently to produce antibody, since nutrients could be diverted from cell proliferation to antibody production in such non-proliferating viable cell culture.

Animals↗
PubMed1997

Subchorial hematoma: a probable cause of reversible nonimmune hydrops fetalis.

Nonimmune hydrops fetails diagnosed at 21 weeks' gestation with profound ascites, hydrothorax, and pericardial effusion receded gradually with regression of a subchorial placental lucencies immediately below the umbilical cord insertion. Careful inspection of the delivered placenta revealed that there was a yellowish lesion of fibrin deposits below the cord insertion site, which resulted from the absorption of hematoma. A subchorial placental hematoma, which detected as a subchorial placental lucencies by ultrasonography, can be a cause of reversible nonimmune hydrops fetalis.

Adult↗
PubMed1997

Anti-thrombogenic effects of 2-hydroxyethylmethacrylate-styrene block copolymer and argatroban in synthetic small-caliber vascular grafts in a rabbit inferior vena cava model.

The high anti-thrombogenicity of 2-hydroxyethylmethacrylate (HEMA)-styrene block copolymer (HS) and argatroban-coated polyurethane tubes in a rabbit inferior vena cava model is reported. Polyurethane tubes (i.d. 3.0 mm, o.d. 4.0 mm, 12.5 cm) were implanted into the inferior vena cavas of 36 rabbits, weighing 2.5 to 3.5 kg, using the sleeve anastomotic technique. The animals were divided into four groups according to the type of prosthesis: uncoated tubes (Group 1, n = 9); HS-coated tubes (Group 2, n = 9); heparin-coated tubes (Group 3, n = 9); and HS/argatroban-coated tubes (Group 4, n = 9). Patency was evaluated at 1 week by ultrasonic flowmetry and microscopic examination of the grafts. Patency rates were 0 percent (0/9) in Group 1; 56 percent (5/9) in Group 2; 67 percent (6/9) in Group 3; and 100 percent (9/9) in Group 4 Groups 2 and 3 animals demonstrated statistically significantly superior patency compared to those of Group 1. Group 4 animals exhibited significantly superior anti-thrombogenicity compared to those of both Groups 1 and 2. Although Group 3 animals had an excellent patency rate, transmission and scanning electron microscopic views showed a thick protein layer and activated platelets adhering to the surface of Group 3 vessels. On the contrary, only a thin protein layer was noted in Group 4 animals. The HS-treated surface combined with the argatroban slow-release system exhibited excellent anti-thrombogenicity under venous-flow conditions.

Anastomosis, Surgical↗
PubMed1997

Synthesis and aromatase-inhibitory activity of imidazolyl-1,3,5-triazine derivatives.

Triamino-substituted 1,3,5-triazine derivatives were synthesized and tested for inhibitory activities against the aromatase of human placental microsomes and the cytochrome P450 side chain cleavage of cholesterol (P450SCC) of pig adrenal mitochondria. The compounds having imidazolyl and tertiary amino groups as substituents in the 1,3,5-triazine ring showed significant aromatase-inhibitory activity. Among them, compounds 17, 23, 26, 27 and 28 were more active than the reference compound, CGS 16949A. The inhibitory activities of these compounds against P450SCC were much weaker than their aromatase-inhibitory activities. These compounds may be regarded as selective aromatase inhibitors.

Adrenal Glands↗
PubMed1997

High-pressure-induced hemolysis of hereditary spherocytic erythrocytes is not suppressed by DIDS labeling.

Hemolytic properties of human erythrocytes in hereditary spherocytosis (HS) were examined under hydrostatic pressure or hypotonic conditions. In the hypotonic buffer, HS erythrocytes were more fragile than normal erythrocytes, and the osmotic fragility was similarly enhanced if both erythrocytes were treated with 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), an anion transport inhibitor. On the other hand, the hemolysis of HS erythrocytes at 200 MPa was almost the same degree as that of normal cells. Upon DIDS treatment, the hemolysis of normal erythrocytes at 200 MPa was suppressed by about 35%, whereas that of HS erythrocytes was not affected. The absence of a suppressive effect of DIDS on high-pressure-induced hemolysis is likely to be HS-specific and may be a reflection of the underlying defects of band 3-cytoskeleton interactions in HS erythrocytes.

4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid↗
PubMed1996

Visualization of slow axonal transport in vivo.

In axons, cytoskeletal constituents move by slow transport. However, it remains controversial whether axonal neurofilaments are dynamic structures in which only subunits are transported or whether filaments assemble in the proximal axon and are transported intact as polymers to the axon terminus. To investigate the form neurofilament proteins take during transport, neurons of transgenic mice lacking axonal neurofilaments were infected with a recombinant adenoviral vector encoding epitope-tagged neurofilament M. Confocal and electron microscopy revealed that the virally encoded neurofilament M was transported in unpolymerized form along axonal microtubules. Thus, neurofilament proteins are probably transported as subunits or small oligomers along microtubules, which are major routes for slow axonal transport.

Adenoviridae↗
PubMed1996

Effects of intracellular pH on high pressure-induced hemolysis of anion transport inhibitor-treated erythrocytes.

Effects of anion transport inhibitors such as 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS) and 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate on hemolysis of human erythrocytes at 200 MPa were examined by changing intracellular pH (7.2-7.9). These inhibitors suppressed the hemolysis at neutral pH but enhanced it at alkaline pH. However, such an enhancement was suppressed by cross-linking of membrane proteins using diamide. From the near-UV CD spectra of band 3 and the relation between hemolysis and anion transport in intact or trypsin-treated erythrocytes, it was found that such hemolytic properties were characterized by the binding of inhibitors to band 3. In addition, spectrin detachment from the erythrocyte membrane by high pressure was considerably suppressed by DIDS treatment at neutral pH, but not by DIDS labeling at alkaline pH. These results suggest that the interaction of the cytoplasmic domain of band 3 with the cytoskeleton, which is induced by the binding of ligands to the exofacial domain of band 3, is dependent on the intracellular pH, i.e., the linking is tightened at neutral pH but relaxed at alkaline pH.

4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid↗
PubMed1996

Cytokines involving gp130 in signal transduction suppressed growth of a mouse hybridoma cell line and enhanced its antibody production.

Three cytokines, interleukin 6 (IL-6), leukaemia inhibitory factor (LIF), and oncostatin M (OSM), that bind to composite receptors including a common signal transducer gp130 suppressed proliferation of a mouse B-cell hybridoma cell line 2E3-O cultured in serum-free medium, while they enhanced antibody production of the cells. The specific growth rate of the cells reduced from 1.0/day for control to 0.6/day for the cultures supplemented with IL-6, LIF, or OSM at 1, 4, or 2 ng/ml, respectively. The antibody productivity increased five-fold when the cells were cultured with IL-6, LIF, or OSM at 1, 25, or 20 ng/ml, respectively. Transforming growth factor beta1(TGF-beta1) similarly suppressed growth of the cells at the concentration of 5 ng/ml, while it did not enhance the antibody production. Cell cycle analysis revealed that IL-6 induced the cells to be arrested at G1 phase of the cell cycle more intensively than TGF-beta1, indicating that IL-6 and TGF-beta1 suppressed the growth through mutually different mechanisms. As a whole, this work suggests that gp130, which is commonly involved in each receptor for IL-6, LIF, and OSM, transduces signals for suppressing proliferation and possibly for enhancing antibody production in the hybridoma cells.

Animals↗
PubMed1996

[Evaluation of reconstruction arc in myocardial SPECT imaging using a cardiac phantom--comparison between 360 degrees and 180 degrees arcs].

In order to investigate the effect of reconstruction arc on myocardial SPECT images, a series of phantom studies was performed with and without plastic chambers simulating perfusion defects using 201Tl and 99mTc. Coefficient of variations (CV) of the counts among the ROIs and defect contrast were evaluated in 360 degrees and 180 degrees images reconstructed from the same 360 degrees projection data. Reconstruction processes were identical for all images. In the absence of defects, the CV of the counts were approximately the same in 360 degrees and 180 degrees images. The CV of the counts in the 360 degrees 201Tl image, among 4 defects located on the anterior, lateral, inferoposterior, and septal walls, was superior to those in the 180 degrees images. In contrast, in the 99mTc images, the CV of the counts among the 4 defects in the 180 degrees image was superior to those of the 360 degrees image. The defect contrast was changed both by the location of the defect and by the reconstruction arc (201Tl, 99mTc). The defect contrast of the 180 degrees images, in both 201Tl and 99mTc experiments, was closer to the true contrast value as calculated by the count ratio between myocardium and defect. Although the defect contrast in the anterior, lateral and septal walls was more emphasized in the 180 degrees images, the defect contrast in the inferoposterior wall was less emphasized in the 180 degrees images compared to the 360 degrees (201Tl, 99mTc).

Heart↗
PubMed1995

Purification and characterization of a non-hemorrhagic metalloprotease from Agkistrodon halys brevicaudus venom.

A non-hemorrhagic metalloprotease (protease L4) was purified from the venom of Chinese Mamushi (Agkistrodon halys brevicaudus) by gel filtration and anion-exchange chromatography. Protease L4 has the molecular weight of 22,000 and its optimum pH was 8.5. The protein was stable in the pH range of 5-9 and below 40 degrees C. The proteolytic activity was inhibited by metal-chelating agents and some metal ions. Calcium ion activated the activity dose-dependently, but had only a minor effect on the thermal and pH stability. L4 showed fibrinogenase activity, hydrolyzing only the A alpha chain of fibrinogen. The protease cleaved preferentially at the N-terminal of Leu and His residues of some peptides.

Agkistrodon↗
PubMed1995

Induction of ductal carcinomas by intraductal administration of 7,12-dimethylbenz(a)anthracene in Wistar rats.

Postpartum Wistar inbred rats (weaned on the 9th puerperal day) were injected intraductally in one mammary gland with 7,12-dimethylbenze (a) anthracene (DMBA) to selectively induce ductal carcinoma. The incidence of ductal hyperplasia increased with time until it peaked at 7 weeks (12/13 animals) and then decreased. Ductal carcinoma first developed at 9 weeks in 3/12 (2 non-invasive and 1 invasive lesion) and the incidence increased with time until invasive ductal tumors were observed in 9/11 at 20 weeks. Tumors developed only in the DMBA-treated mammary glands and no systemic effects of the carcinogen were observed. Degeneration and detachment of epithelioglandular cells were seen here and there in the ducts and terminal ducts, and epithelioglandular cells proliferated in terminal duct until 2 weeks. Residual trace DMBA powder was detected in terminal ducts and the epithelioglandular layer until 7 weeks. This trace DMBA was considered to be the cause of the development of atypical epithelial cells, inducing ductal carcinomas.

9,10-Dimethyl-1,2-benzanthracene↗