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Biomedical subjects

S Tang

Publications and source records attributed to S Tang.

At least 235 records · Page 13Linked to original sources
PubMed1993

[Seroepidemiological study on hepatitis C virus infection among blood donors from various regions in China].

A total of 2,273 blood donors from various regions in China were tested for serum anti-HCV antibodies in a seroepidemiological study. The prevalence of anti-HCV in Volunteer blood donors was 0-1.10%, which was lower than that in professional blood donors from Liaoning and Anhui Provinces (1.49% and 3.14%, respectively), whereas the positivity rate of anti-HCV was as high as 30.13% in the professional blood donors from Hebei Province and 31.86% in those from Inner Mongolia Autonomous Region. The prevalence of anti-HCV was significantly higher in the blood donors with history of hepatitis and abnormal ALT levels than those without hepatitis and with normal ALT. Plasma donation was the main cause of HCV infection. However, the prevalence of anti-HCV showed no significant sex and age differences even though the anti-HCV activity profile showed geographic difference.

Adolescent↗
PubMed1993

Study on application of the silicone oil in the reattachment of complicated retinal detachment.

Sixty eyes with complicated retinal detachment were treated by vitrectomy combined with silicone oil tamponade (27 eyes of PVR grade D, 15 eyes of giant retinal tears with posterior flat folded-over, 13 eyes of posterior or macular hole, 5 eyes of traumatic PVR). After 3-24 months follow-up, the study showed retinal anatomic reattachment in 48 eyes in which the visual acuity of 32 eyes was 0.05 or better. The authors consider that vitrectomy and peeling make the silicone oil tamponade perfect the vitreous surgery and improve the successful rate. The theory of silicone oil tamponade, the indications, advantages and disadvantages of silicone oil tamponade are discussed in this paper.

Adolescent↗
PubMed1993

[The changes in the levels of angiotensins and angiotensin converting enzyme and effects of captopril in patients with hypoxic pulmonary hypertension].

The levels of angiotensin I, II (ATI, II) and angiotensin converting enzyme (ACE) were measured in 28 patients with hypoxic pulmonary hypertension and 23 normal individuals. The effects of captopril were observed in 8 of 28 patients. The results showed that the average level of ATII in the patients was significantly higher than that in normal control (P < 0.05), but the average level of ACE in the patients was significantly lower than that in the controls (P < 0.05). In 8 patients, after the use of captopril, an ACE inhibitor, for two weeks, the levels of ATII, ACE and mean pulmonary arterial pressure (mPAP) were markedly decreased (P < 0.05). But the other 20 patients treated with antibiotics, antiasthmatics, diuretics and oxygen therapy for two weeks did not show any alterations in the levels of ATII, ACE and mPAP.

Aged↗
PubMed1992

Lack of human immunodeficiency virus type 1 (HIV-1) replication and accumulation of viral DNA in HIV-1-infected T cells blocked in cell replication.

Human immunodeficiency virus type 1 (HIV-1) infection of the CD4+ SupT and CEM cell lines, blocked in cell replication by the polymerase alpha inhibitor aphidicolin (APC), was studied. The APC-treated cells showed a lack of viral production, but the presence of single cell killing. High levels of unintegrated viral DNA forms were found in the infected APC-treated cells as compared with untreated cells. Moreover, an increased rate of viral replication occurred in the remaining viable cells following removal of APC. The results indicate that HIV-1 entry and reverse transcription can take place in cells blocked in the S phase of the cell cycle. Replication of infectious progeny virions appears to require de novo cell division. Finally, accumulation of viral DNA in cells during APC treatment can result in cytopathological effects and subsequent enhancement of virus production.

Aphidicolin↗
PubMed1992

[Changes in plasma glucagon concentration in patients with diabetes mellitus and its clinical significance].

To investigate the role of glucagon in the pathogenesis of diabetes, we observed change of plasma glucagon concentrations during glucose loading in normal subjects and patients with impaired glucose tolerance (IGT) and non-insulin dependent diabetes mellitus (NIDDM) using specific radioimmunoassay. The results showed that the fasting plasma glucagon levels in patients with IGT and NIDDM were similar to those of the normal subjects. The nadir of plasma glucagon level in the normal control occurred at 1-h after glucose loading and the changes of glucagon, glucose and insulin levels synchronized; but peaks of plasma glucose and insulin levels in the IGT and NIDDM patients were delayed at 2-h after glucose loading with the lowest glucagon level at 3-h. It was suggested that there were relative hyperglucagonemia and decrease of sensitivity of islet A cell to glucose in IGT and NIDDM patients. The present study also showed that hyperglucagonemia is related to the reduction of insulin secretion and might play an important role in the development of postprandial hyperglycemia in NIDDM.

Aged↗
PubMed1992

[Thixotropic properties of whole blood in children with congenital heart disease].

The thixotropic parameters of whole blood in two groups of children with congenital heart disease (CHD) were measured. Group 1. cyanotic heart disease (CCHD), 20 cases; Group 2. acyanotic heart disease (ACHD), 30 cases. Fifty healthy children were controls matched with the patients in sex and age. Their thixotropic parameters were compared; the paired t-test was used. In the children with CCHD, the hematocrit (HCT), the yield stress (tau 0) the Newtonian contribution of viscosity (mu), the equilibrium value of the structural parameter (A), the apparent viscosity at 2.37 sec-1 (eta s) and the Non-Newtonian contribution of viscosity (eta s-mu) were significantly higher than those in corresponding control groups. In the children with ACHD, only the values of tau 0, eta s-mu, and eta s were higher than those in control groups. All of the thixotropic parameters in CCHD group were significantly higher than those in ACHD group. Thus we described quantitatively CHD in terms of thixotropy of blood. The thixotropic parameters of blood could be used as indexes of severity for pathologic changes of CHD.

Adolescent↗
PubMed1992

[A patient with severe ventricular tachycardia who was saved by prolonged extracorporeal lung and heart assist].

A 47-y-o man had been suffering from cardiac failure due to refractory ventricular tachycardia (VT) after myocardial infarction. He underwent resection of the left ventricular aneurysm and cryocoagulation of the arrhythmogenic foci. On the 2nd post-operative day, VT often recurred in spite of repeated cardioversion and drug therapy, and threatened his life, even under IABP. Therefore, a veno-arterial bypass route was made and extracorporeal lung and heart assist, ECLHA, was started with a heparin bonded Maxima lung on the following day. Even under ECLHA, VT continued to recur. Cryocoagulation of the VT foci was tried again, without immediate success. A record high dose of beta-blockers, given under the circulatory support by ECLHA, stopped VT on the following day. The patient was weaned from the ECLHA circuit 12 days after the first operation, then from IABP on the 14th day. During the 10 day course of surgeries and ECLHA, the patient had almost 100 defibrillations. But for ECLHA, we may say that the patient couldn't have survived two open heart surgeries, administration of a great amount of beta-blockers, and repeated cardiac arrest without neurological sequelae.

Adult↗
PubMed1992

Lymphocytes, macrophages and HLA-DR expression in vitreal and epiretinal membranes of proliferative vitreoretinopathy. An immunohistochemical study.

Proliferative vitreoretinopathy (PVR) as a severe complication of retinal detachment and also the main cause of failure in its treatments is not yet completely understood. In the present study, we examined 16 PVR epiretinal membrane specimens removed during vitrectomy from 16 patients with complicated rhegmatogenous retinal detachment by immunostaining of cryosections. We looked for the presence of lymphocytes and detected helper/inducer T lymphocytes (CD4+), suppressor/cytotoxic T lymphocytes (CD8+), B lymphocytes (CD22+), macrophages (CD68+), and HLA-DR expression. The results showed that 6 out of 16 specimens reacted positively with monoclonal antibody against the helper/inducer T cell subset (38%), 5 out of 16 reacted positively with a suppressor/cytotoxic T cell-specific monoclonal antibody (31%), 7 out of 16 reacted with B cell-specific monoclonal antibody (44%), and 14 out of 16 were positive for macrophages and HLA-DR (88%). Nevertheless, we think that the invasion of macrophages and lymphocytes into the membranes is not the cause for the pathogenesis, but a secondary event that might further complicate the course of the disease.

Antibodies, Monoclonal↗
PubMed1992

[Tardy epilepsy--clinical analysis of 292 cases].

292 cases of tardy epilepsy were studied. 242 cases of them had undertaken CT scanning, these results were compared with the results of EEG the patients with local seizures or local abnormalities on EEG had a significantly high abnormality rate on the CT scans. In 114 cases (39%) the causes of the seizures were due to head injury, cerebrovascular disease, encephalitis or meningoencephalitis, brain tumor, or excessive alcoholic drinking. The authors were of the opinion that although no relation could be found between the history and abnormal findings on CT scans, the patients with tardy epilepsy should undertake CT scanning early.

Adult↗
PubMed1991

[Isolation and purification of alpha fetal protein utilizing Act-Ultrogel AcA 22 affinity chromatography].

In the present communication, we report a method for the isolation and purification of alpha FP from fetal corpse homogenate in a single-step procedure using Act-Ultrogel AcA 22 affinity chromatogrpahy column. This method proves to be better than the conventional method in simplicity, fastness and validity with recovery, purity and immunoreactivity. The immunoreactivity of the purified alpha FP labelled by the method of Hunter and Greenwood (1962) was 50-60% (B/T%). This was similar to alpha FP standard (WHO). The results indicate that the 125I-alpha FP is suitable for alpha FP radioimmunoassay.

Chromatography, Affinity↗
PubMed1991

[Study on the mechanisms and influential factors of intrauterine infection of hepatitis B virus].

Molecular hybridization methods were used to study the intrauterine HBV infection in induced abortions. HBV-DNA was detected not only in fetal liver, but also in spleen, Pancreas, kidney and placenta. The results suggested that HBV might probably pass through placental barrier or infect the placenta leading to the intrauterine HBV infection. Even a possible vertical transmission of HBV via the germ line might exist. However, it might not be the main way by which the maternal blood entered fetal circulation through placental leakage leading to intrauterine infection of HBV. In maternal blood, HBeAg positivity, especially also positive for HBV-DNA was important for intrauterine infection. But maternal anti-HBc antibody may not be able to suppress HBV antigens expression and HBV replication in fetal tissue.

DNA, Viral↗
PubMed1991

[Study on thixotropic parameters of whole blood from healthy children].

With low shear-30 rheometer, the authors used a modified protocol to measure thixotropic parameters of whole blood from 200 healthy newborns and children, so as to suggest the normal range of blood thixotropic parameters in four age groups, namely, the newborns, 1 year- group, 5 year- and 10-15 year group. The results demonstrated that the thixotropic parameters in the newborn group were higher than those in other three groups. However, these thixotropic parameters in the group 1-10 years were lower than those in the 10-15 year group. No significant sex differences were noted among the fore three groups, but sex differences appeared obviously in the 10-15 year group. It implied that the changes relying on the age is consistent with children's growth, development and physiologic activities.

Adolescent↗
PubMed1990

[Pancreatic glucagon receptor radioassay with liver cell plasma membrane in normal and streptozotocin-induced diabetic rats].

Pancreatic glucagon receptor radioassay has been developed with rat liver cell plasma membrane prepared with sucrose gradient centrifugation. 125I-glucagon was prepared in this laboratory and the separation of B/F was done by low speed centrifugation with addition of PEG (W/V, 20%) and normal human serum. The specific binding of 125I-glucagon was 25%-35% and nonspecific binding was less than 5%. Reproducibility within and between- assay was 6.83% and 8.40% (CV%), respectively. Binding characteristics of glucagon receptor of normal and streptozotocin-induced diabetic rats were determined by using two methods, receptor dilution and conventional competitive binding assay. The results showed that there were no marked differences concerning high and low binding affinities between normal and diabetic rats, but the concentration of binding sites for both high and low affinity receptors was significantly higher for diabetic rats than normal rats. The possible mechanisms for the above results have been discussed.

Animals↗